Emre AVCİ, Gülçin Alp AVCI, Dursun Ali KOSE, Asiye Asli EMNİYET, Menderes SUİCMEZ

Rumex crispus ve Rumex cristatus’un uçucu yağlarının in vitro antimikrobiyal ve antioksidan aktiviteleri ile GC/MS analizi

B u çalışmada Çorum ili ve çevresinden toplanan Rumex crispus ve Rumex cristatus’un uçucu yağlarının antimikrobiyal ve antioksidan aktiviteleri ile GC/MS analizleri araştırıldı. Etanol ve saf su ile hazırlanan örneklerin uçucu yağlarının toplam antioksidan statüsü spektrofotometrik olarak ölçüldü. Antimikrobiyal çalışmalarda Staphylococcus aureus ATCC 25923, Eschrechia coli ATCC 25922, Enterecoccus faecalis ATCC 29212 ve Candida albicans ATCC 10231 türleri kullanıldı. Örneklerin antimikrobiyal özellikleri disk ve agar-kuyu yöntemi ile belirlendi. Tüm Rumex cristatus uçucu yağlarının total antioksidan miktarı, Rumex crispus’a göre daha yüksek bulundu. Antimikrobiyal aktivite testlerinde Rumex crispus ve Rumex cristatus uçucu yağlarının test mikroorganizmalarının gelişimini farklı derecelerde inhibe ettiği gözlendi. Etanol ile elde edilen uçucu yağların kloroforma göre daha etkin olduğu tanımlandı

Anahtar Kelimeler:

Rumex crispus, Rumex cristatus, uçucu yağ, antioksidan aktivite, antimikrobiyal aktivite, GC-MS

In vitro antimicrobial and antioxidant activities and GC/MS analysis of the essential oils of Rumex crispus and Rumex cristatus

In this study, was investigated in vitro antimicrobial and antioxidant activities and GC/MS analysis of the essential oil of Rumex crispus and Rumex cristatus collected from Corum province and around. Total antio- xidant status of essential oils prepared with ethanol and pure water were measured spectrophotometrically. Staphylococcus aureus ATCC 25923, Eschrechia coli ATCC 25922, Enterecoccus faecalis ATCC 29212 and Can- dida albicans ATCC 10231 were used to the antimicrobial studies. The antimicrobial properties of samples were determinate with the disk diffusion and agar-well method. The total antioxidant status in the essential oils of all Rumex cristatus contained high levels was determinate according to Rumex crispus. The developing of test microorganisms of the essential oils of all Rumex crispus and Rumex cristatus extracts have been observed to inhibit varying degrees on the antimicrobial activity test. Furthermore, extracts obtained with ethanol is more effective according to chloroform extracts and more efficient results which have been identified. Enterecoc- cus faecalis ATCC 29212, Escherichia coli ATCC 25922 Staphylococcus aureus ATCC 25923 and fungal culture of Candida albicans ATCC 10231. The diphenic acid derivatives were found the content of two Rumex sp. essential oils with GC/MS analysis.

Keywords:

Rumex crispus, Rumex cristatus, essential oils, antioxidant activity, antimicrobial activity, GC-MS,

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