Karbon Pasta Biyosensöründe Dönüştürücü Olarak Kullanılan Mantar Dokusunun Farklı Bir Yönü

F enolik bileşiklerin belirlenmesi için enzimatik bir karbon elektrot macunu geliştirilmiştir. Elektrot malzemesi parafin ve canlı ortamda polifenol oksidaz PPO içeren ham mantar doku ile birlikte tek dağılımlı monodisperse grafit tozunun karıştırılması ile hazırlanmıştır. Bileşenleri arasındaki oran karbon - doku parafin , 0.1 M sodyum fosfat tamponu, pH 7, ve 50 mV/s tarama hızında SCV Stare Case Voltammetry teknikleri kullanılarak araştırılmıştır. Türev voltamogramlar biyosensör yanıtını değerlendirmek için kullanılır. Analitik performans karbon tozu granülometrisi ile yakından ilgilidir. Karbon tozu granülometrisi 0,09-0,071 mm iken, arka akım ve hassasiyeti 1.57 mA / ppm ile ilgili olarak en iyi sonuçlar elde edildi. Ayrıca mantar gövdesinin ayrılmış dört bölümünden alınan PPO aktivitesi incelenmiştir. Enzimatik reaksiyonun kinetiği Michaels-Menten mekanizmasına göre sonuçlanmıştır. Biyosensör yanıtı, farklı fenolik bileşiklere karşı test edilmiştir. Hidrokinon için biyosensör duyarlılığı ile ilgili en iyi sinyal S = 2.04 mA / ppm , korelasyon katsayısı R2 = 0,9997 ve algılama sınırı 0.7 ppm elde edilmiştir ve diğer çalışılan bileşikler şu sırada verilmektedir: hidrokinon> katekol > fenol> m-kresol> 4-klorofenol> p-kresol> 4-nitrofenol> 3-nitrofenol

Anahtar Kelimeler:

Fenolik bileşik, karbon pasta biyosensör, PPO, ham doku, voltametri

Different Part of Mushroom Tissue Used as Modifier in Carbon Paste Sensor

An enzymatic carbon paste electrode for determination of phenolic compounds is developed. The electrode material was prepared by mixing the monodisperse graphite powder with paraffin and with crude mushroom tissue which contains polyphenol oxidase PPO in its living environment. The ratio between components carbon - tissue - paraffin , was investigated using SCV Stare Case Voltammetry techniques in 0.1 M sodium phosphate buffer, pH 7, scan rate 50 mV/s. The derivative voltammogrammes are used to evaluate the biosensor response. The analytical performance was closely related with the carbon powder granulometry. The optimal results regarding the background current and sensitivity 1.57 mA/ppm were obtained when carbon powder granulometry was 0.09-0.071 mm. It was also studied the activity of the PPO taken from four separated sections of mushroom body. Kinetic of enzymatic reaction resulted according to the Michaels–Menten mechanism. The biosensor response was tested toward different phenolic compounds. The best signal regarding the sensitivity of biosensor S=2.04 mA/ppm , correlation coefficient R2= 0.9997 and detection limit 0.7 ppm was obtained for hydroquinone and the other studied compounds are listed in these order: hydroquinone > catechol > phenol > m-cresol > 4-chlorophenol > p-cresol > 4-nitrophenol > 3-nitrophenol.

Keywords:

Phenolic compound, carbon paste biosensor, PPO, crude tissue, Voltammetry,

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