Bisfenol A’nın BPA Drosophila Melanogaster’de Gelişim ve Üreme Üzerine Etkileri

B u çalışmanın amacı, endokrin bozucu kimyasallardan EBK biri olan BPA’nın Drosophila melanogaster’in gelişimi ve üremesi üzerine etkilerini araştırmaktır. D. melanogaster larvalarına 0.1 mg/L, 1 mg/L ve 10 mg/L BPA uygulanmıştır. Larvadan pupaya, pupadan ergine geçiş yüzdeleri ve süreleri ve ortalama yavru döl sayıları belirlenmiştir. Larvadan pupaya, pupadan ergine geçiş yüzdelerinde anlamlı bir değişim gözlenmemiştir p > 0.05 . Ancak BPA uygulamaları ile hem ortalama pupalaşma hem de ortalama erginleşme süreleri gecikmiştir p

Anahtar Kelimeler:

Bisfenol A BPA, Drosophila melanogaster, Gelişim, Ortalama yavru döl sayısı

Developmental and Reproductive Effects of Bisphenol A Bpa in Drosophila Melanogaster

The aim of this study was to investigate the effects of BPA that is one of the endocrine disrupting chemicals EDCs on the development and reproduction of Drosophila melanogaster. Larvae of D. melanogaster were exposed to 0.1 mg/L, 1 mg/L and 10mg/L BPA. The percentages and times of transition from larvae to pupae and from pupae to adults and the mean offspring number were determined. No differences were found in the transition percentages from larvae to pupae and from pupae to adults p > 0.05 . However, it was found that both the mean pupation and the mean maturation times were delayed with BPA exposures p

Keywords:

Bisphenol A BPA, Drosophila melanogaster, Development, Mean offspring number,

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MM. Watts, D. Pascoe, K. Carroll, Chronic exposure to 17α-ethinylestradiol and bisphenol A effects on development and reproduction in the freshwater invertebrate Chironomus riparius (Diptera:Chironomidae), Aqua. Toxicol., 55 (2001) 113.
JL. Martinez-Guitarte Planello, G. Morcillo, The endocrine disruptor bisphenol A increases the expression of HSP70 and ecdysone receptor genes in the aquatic larvae of Chironomus riparius, Chemosphere, 71 (2008) 1870.
MFL. Lemos, CAM. Van Gestel, AMVM. Soares, Developmental toxicity of endocrine disrupters bisphenol A and vinclozolin in a terrestrial isopod, Arch. Environ. Contam. Toxicol., 59 (2010) 274.
H. Fromne, T. Küchler, T. Otto, K. Pilz, J. Müler, A. Wenzel, Occurance of phthalates and bisphenol A and F in the environment, Water Res., 36 (2002) 1429.
C. Sonnenschein, AM. Soto, An updated review of environmental estrogen and androgen mimics and antagonists, J. Steroid Biochem. Mol. Biol., 65 (1998) 143.
FS. Vom Saal, C. Hughes, An extensive new literature concerning low-dose effects of bisphenol A shows the need for a new risk assessment, Environ. Health Perspect., 113 (2005) 926.
T. Hahn, K. Schenk, R. Schulz, Environmental chemicals with known potential affect yolk protein content in the aquatic insect Chironomus riparius, Environ. Pol., 120 (2002) 525.
J. Oehlmann, U. Schulte-Oehlmann, Endocrine disruption in invertebrates, Pure Appl. Chem., 75 (2003) 2207.
MM. Watts, D. Pascoe, K. Carroll, Exposure to 17a-ethinylestradiol and bisphenol A—effects on larval moulting and mouthpart structure of Chironomus riparius, Ecotoxicol. Environ. Saf., 54 (2003) 207.
MFL. Lemos, CAM. Van Gestel, AMVM. Soares, Reproductive toxicity of the endocrine disrupters vinclozolin and bisphenol A in the terrestrial isopod Porcellio scaber (Latreille, 1804), Chemosphere, 78
H. Segner, K. Caroll, M. Fenske, CR. Janssen, G. Maack, D. Pascoe, C. Schafers, GF. Vandenbergh, M. Watts, A. Wenzel, Identification of endocrine-disrupting effects in aquatic vertebrates and invertebrates: report from the European IDEA project, Ecotoxicol. Environ. Saf. 54 (2003) 302.
MFL. Lemos, CAM. Van Gestel, AMVM. Soares, Endocrine disruption in a terrestrial isopod under exposure to bisphenol A and vinclozolin, J. Soils Sediments, 9 (2009) 492.
N. Izumi, R. Yanagibori, S. Shigeno, J. Sajiki, Effects of bisphenol A on the development, growth and sex ratio of the housefly Musca domestica, Environ. Toxicol. Chem., 27 (2008) 1343.
TH. Hutchinson, Reproductive and developmental effects of endocrine disrupters in invertebrates: in vitro and in vivo approaches, Toxicol. Letters, 131 (2002) 75.
J. Oehlmann, U. Schulte-Oehlmann, J. Bachmann, M. Oetken, I. Lutz, W. Kloas, TA. Ternes, Bisphenol A induces superfeminization in the ramshorn snail Marisa cornuarietis (Gastropoda: Prosobranchia) at environmentally relevant concentrations, Environ. Health Perspect., 114 (2006) 127.
H. Li, D. Harrison, G. Jones, D. Jones, RL. Cooper, Alterations in development, behavior, and physiology in Drosophila larva that have reduced ecdysone production, J. Neurophysiol., 85 (2001) 98.
KD. Baker, JT. Warren, CS. Thummel, LI. Gilbert, DJ. Mangelsdorf, Transcriptional activation of the Drosophila ecdysone receptor by insect and plant ecdysteroids, Insect Biochem. Mol. Biol., 30 (2000) 1037.
K. King-Jones, CS. Thummel, Nuclear receptors – A perspective from Drosophila, Nature Reviews/ Genetics, 6 (2003):311.
T. Kozlova, CS. Thummel, Steroid regulation of postembryonic development and reproduction in Drosophila, TEM, 11 (2000) 276.
E. Zou, M. Fingerman, Effects of estrogenic xenobiotics on molting of the water flea Daphnia magna, Ecotoxicol. Environ. Saf., 38 (1997) 281.
L. Dinan, P. Bourne, P. Whiting, TS. Dhadialla, TH. Hutchinson, Screening of environmental contaminants for ecdysteroid agonist and antagonist activity using the Drosophila melanogaster B-II cell in vitro assay, Environ. Toxicol. Chem., 24 (2001) 2038.
JG. Sorensen, V. Loeschcke, Effects of relative emergence time on heat stress resistance traits, longevitiy and hsp70 expression level in Drosophila melanogaster, J. Thermal Biol., 29 (2004) 195.
FA. Lints, CV. Lints, Relationship between growth and aging in Drosophila, Nature, 229 (1971) 86.
V. Bindhumol, KC. Chitra, PP. Mathur, Bisphenol A induces reactive oxygen species generation in the liver of male rats, Toxicology, 188 (2003) 117.
KC. Chitra, C. Latchoumycandane, PP. Mathur, Induction of oxidative stress by bisphenol A in the epididymal sperm of rats, Toxicology, 185 (2003) 119.
SH. Cho, MH. Choi, OS. Kwon, WY. Lee, BC. Chung, Metabolic significance of bisphenol A-induced oxidative stres in rat urine measured by liquid chromatography-mass spectrometry, J. Apll. Toxicol., 29 (2008) 110.
H. Kabuto, S. Hasuike, N. Minagawa, T. Shishibori, Effect of bisphenol A on the metabolism of active oxygen species in mouse tissues, Environ. Res., 93 (2003) 31.
T. Obata, S. Kubota, Formation of hydroxyl radicals by environmental estrogen-like chemicals in rat striatum, Neurosci. Lett., 296 (2000) 41.
D. Roy, M. Palangt, CW. Chen, RD. Thomas, J. Colerangle, A. Atkinson, ZJ. Yan, Biochemical and molecular changes at the cellular level in response to exposure to environmental estrogen-like chemicals, J. Toxicol. Environ. Health, 50 (1997) 1.
G. Morrow, RM. Tanguay, Heat shock proteins and aging in Drosophila melanogaster, Seminars in Cell & Dev. Biol., 14 (2003) 291.
LG. Neven, Physiological responses of insects to heat, Postharvest Biol. Technol., 21 (2000) 103.
SP. Roberts, ME. Feder, Natural hyperthermia and expression of the heat shock protein Hsp70 affect developmental abnormalities in Drosophila melanogaster, Oecologia, 121 (1999) 323.
RA. Krebs, ME. Feder, Hsp 70 and larval thermotolerance in Drosophila melanogaster: how much is enough and when is more too much?, J Insect Physiol., 44 (1998) 1091.
SM. Lee, SB. Lee, CH. Park, J. Choi, Expession of heat shock protein and hemoglobin genes in Chironomus tetans (Diptera, Choronimidae) larvae exposed to various environmental pollutants: a potential biomarker of freshwater monitoring, Chemosphere, 65 (2006) 1074.
SJ. Brennan, CA. Brougham, JJ. Roche, AM. Fogarty, Multi-generational effects of four selected environmental oestrogens on Daphnia magna, Chemosphere, 64 (2006) 49.
M.H.I. Comber, T.D. Williams, K.M. Stewart, The effects of nonyl phenol on Daphnia magna, Water Res., 27
WS. Baldwin, SE. Graham, D. Shea, GA. LeBlanc, Metabolic androgenization of female Daphnia magna by the xenoestrogen 4-nonylphenol, Environ. Toxicol. Chem., 16 (1997) 1905.