A Comparison of Two Different Diets and Their Restricted Groups in Relation to Developmental Time and Viability in Drosophila melanogaster

Effects of dietary conditions on many life history traits have been studied for a long time with Drosophila. However, many laboratories use different diets and techniques, which make it difficult to compare the results obtained from various studies. For this reason an optimization protocol for dietary restriction DR studies seems to be necessary and in fact recently an optimization protocol published by Bass et al. [1]. In our study we compared the standard diet S that we use in our laboratory and the proposed diet P with the restricted groups of both diets. In our work, differences between two different dietary protocols with respect to egg-to-adult viability and developmental time were investigated. The P diet did not show any significant differences between the groups with respect to developmental time. On the contrary, the S diet showed significant differences in the yeast and yeast + sugar restricted groups when compared with the respective control and sugar restricted groups.

___

  • Bass, T.M., Grandison, R.C., Wong, R., Martinez, P., Partridge, L., Piper, M.D.W., Optimization of dietary restriction protocols in Drosophila, J. Gerontol. Biol. Sci., 62(10), 1071, 2007.
  • Heilbronn, L.K., Ravussin, E., Calorie restriction and aging: review of the literature and implications for studies in humans., Am. J. Clin. Nutr., 78, 361, 2003.
  • Chippindale, A.K., Leroi, A.M., Kim, S.B., Rose, M.R., Phenotypic plasticity and selection in Drosophila life-history evolution. I. Nutrition and the cost of reproduction, J. Evol. Biol., 6, 171, 1993.
  • Pletcher, S.D., Macdonald, S.J., Marguerie, R., Certa, U., Stearns, S.C., Goldstein, D.B., Partridge, L., Genome-wide transcript profiles in aging and calorically restricted Drosophila melanogaster, Cur. Biol., 12, 712, 2002.
  • Önder, B.Ş., Yılmaz, M., Özsoy, E.D., Larval food restriction and longevity: Effects on the age specific mortality and mean longevity in natural populations of Drosophila melanogaster and its sibling D. simulans, Hacettepe J. Biol. Chem., 37(1), 1, 2009.
  • Mockett, R.J., Cooper, T.M., Orr, W.C., Sohal, R.S., Effects of caloric restriction are species-specific, Biogerontology, 7, 157, 2006.
  • Mair, W., Piper, M.D., Partridge, L., Calories do not explain extension of lifespan by dietary restriction in Drosophila. PLoS Biol. 7, e223, 2005.
  • Chapman, T., and Partridge, L., Female fitness in Drosophila melanogaster: an interaction between the effect of nutrition and of encounter rate with males. Proc. R. Soc. Lond. B Biol. Sci., 263, 755, 1996.
  • Kapahi, P., Zid, B.M., Harper, T., Koslover, D., Sapin, V., Benzer, S., Regulation of lifespan in Drosophila by modulation of genes in the TOR signaling pathway. Curr. Biol. 14, 885, 2004.
  • Bross, T.G., Rogina, B., Helfand, S.L., Behavioral, physical, and demographic changes in Drosophila populations through dietary restriction. Aging Cell., 4, 309, 2005.
  • Zwaan, B.J., Bijlsma, R., Hoekstra, R.F., On the developmental theory of ageing. I. Starvation resistance and longevity in Drosophila melanogaster in relation top re- adult breeding conditions, Heredity, 66, 29, 1991.
  • Partridge, L. and Fowler, K., Direct and correlated responses to selection on at age reproduction in Drosophila melanogaster. Evolution, 46, 76, 1992.
  • James, A.C., and Partridge, L., Thermal evolution of rate of larval development in Drosophila melanogaster in laboratory and field populations. J. Evol. Biol. 8, 315, 1995.
  • Zwaan, B.J., Bijlsma, R., Hoekstra, R.F., On the developmental theory of ageing. II. The effect of developmental temperature on longevity in relation to adult body size in Drosophila melanogaster. Heredity, 68, 123, 1992.
  • Önder, B.Ş. and Yılmaz, M., The effect of dietary restriction on developmental time in Drosophila melanogaster and its sibling D. simulans, Dros. Inf. Serv., 92, 95, 2009.
  • Tu, M.P., and Tatar, M., Juvenile diet restriction and the aging and reproduction of adult Drosophila melanogaster, Aging Cell., 2, 327, 2003.
  • Bozcuk, A.N., The effects of some genotypes on longevity of adult Drosophila. Exp. Gerontol., 13, 279, 1978.
  • Ashburner, M., Drosophila. A Laboratory Manual. New York: Cold Spring Harbor Press, pp. 399, 1989.
  • Edgecomb, R.S., Harth, C.E., Schneiderman, A.M., Regulation of feeding behavior in adult Drosophila melanogaster varies with feeding regimen and nutritional state. J. Exp. Biol., 197, 215, 1994.
  • Carvalho, G.B., Kapahi, P., Benzer, S., Compensatory ingestion upon dietary restriction in Drosophila melanogaster. Nat. Methods., 2, 813, 2005.
  • Piper, M.D. and Partridge, L., Dietary restriction in Drosophila: delayed aging or experimental artifact? PLoS Genet., 3, e57, 2007.