Nutritional Factors Effecting Rhamnolipid Production by a Nosocomial Pseudomonas aeruginosa

Different Pseudomonas strains were isolated and grown on the basal medium and inspected for rhamnolipid biosurfactant production. They were incubated in a rotary shaker for 8 days at 30 oC and at 150 rev min-1. In order to increase the rhamnolipid production, 7 different carbon sources, including glucose, glycerol, mannitol, hexadecane, fructose, maltose and lactose were used. Varying concentrations of carbon sources for maximum rhamnolipid concentration were also determined. Different nitrogen sources in the basal medium, such as NH4 2SO4, NH4H2PO4, NH4NO3, KNO3, NaNO3 and peptone were tested. Resulting rhamnolipid concentration was 589.3 mg/L, when 2% mannitol was used as the carbon source and 2.0 g/L NaNO3 as the nitrogen source.

Keywords:

Pseudomonas aeruginosa, rhamnolipid biosurfactant, nosocomial bacteria,

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