Recognition and fast separation of Paclobutrazol pesticides via molecular imprinted silica nanoparticles
In this study, molecularly imprinted silica nanoparticles was prepared with surface imprinting method for specific recognition with high affinity and separation of paclobutrazol in honey samples. To prepare molecularly imprinted silica nanoparticles, Paclobutrazol was used as template molecule, 2-hydroxyethyl methacrylate as monomer, 1,2-ethyleneglycoldimethacrylate as crooslinking agent. Furthermore, MIP SNPs were synthesized by single electron transfer living radical polymerization. The prepared nanoparticles can be easily separated and collected by ultrasantrifuge with 140000 rpm and exhibited highly selectivity to template molecules. All rebinding studies showed that the MIP SNPs had excellent recognition towards Paclobutrazol. The recoveries of PB in the spiking honey samples changed from 98.7% to 99.9% with the relative deviation from 8.62% to 4.92% According to all results, MIP SNPs can be good alternative for selective recognition and efficient separation of pesticides in real samples.
Keywords:
: single electron transfer living radical polymerization, Paclobutrazol, pesticides molecularly imprinted,
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- [1] Kmellar, B., Abranko, L., Fodor, P., Lehotay, S. J., “Routine approach to qualitatively screening 300 pesticides and quantification of those frequently detected in fruit and vegetables using liquid chromatography tandem mass spectrometry (LC-MS/MS)”, Food Addit. Contam. Part A-Chem. Anal. Control Expo Risk Assess, 24: 1415-1430, (2010).
- [2] Bordagary, A., Garcia-Arrona, R., Millan, E., “Development and application of a screening method fortriazole fungicide determination in liquid and fruit samples using solid-phase microextraction and HPLC-DAD.”, Analytical Methods, 10: 2565-2571, (2013).
- [3] Cheng, Y., Dong, F., Liu, X., Xu, J., Li, J., Chen, X., Li, Y., Wu, X., Zheng, Y., “Stereoselective separation and determination of the triazolefungicide propconazole in water, soil and grape by normal phase HPLC.”, Analytical Methods, 3: 755-761, (2013).
- [4] Farajzadeh, M.A., Mogaddam, M.R.A., Ghorbanpour, H., “Development of a new microextraction method based on elevated temperature dispersive liquid-liquid microextraction for determination of triazole pesticides residues in honey by gas chromatography-nitrogen phosphorus detection.”, Journal of Chromatography A, 1347: 8-16, (2014).
- [5] Fu, G., He, H., Chai, Z., Chen, H., Kong, J., Wang, Y., Jiang, Y., “Enhanced Lysozyme Imprinting Over Nanoparticles Functinalized with Carboxyl Groups for Noncovalent Template Sorption”, Anal. Chem., 83: 1431-1436, (2011).
- [6] Turan, E., Şahin, F., “Molecularly imprinted biocompatible magnetic nanoparticles for specific recognition of Ochratoxin A”, Sensors and Actuators B:Chem., 227: 668-676, (2016).
- [7] Chen, T., Shao, M., Xu, H., Zhuo, S., Liu, S., Lee, S-T., “Molecularly imprinted polymer-coated silicon nanowires for protein specific recognition and fast separation”, J of Mater. Chem., 22: 3990-3996, (2012).
- [8] Stöber, W., Fink, A., Bohn, E., “Controlled growth of monodisperse silica spheres in the micron size range”, J. Colloid Inter. Sci., 26: 62-69, (1968).
- [9] Fatoni, A., Numnuam, A., Kanatharana, P., Limbut, W., Thavarungkul, P., “A novel molecularly imprinted chitosan-acrylamide, graphene,ferrocene composite cryogel biosensor used to detect microalbumin”, Analyst, 139: 6160-6167, (2014).
- [10] Li, L., He, X., Chen, L., Zhang, Y., “Preparation of Core-shell Magnetic Molecularly Imprinted Polymer Nanoparticles for Recognition of Bovine Hemoglobin”, Chem. Asian J., 4: 286-293, (2009).
- [11] Xiao, D., Wang, C., Dai, H., Peng, J., He, J., Zhang, K., Kong, S., Qiu, P., He, H., “Applications of magnetic surface imprinted materials for solid phase extraction of levofloxacin in serum samples”, J. Mol. Recognit., 28: 277-284, (2015).
- Yayın Aralığı: Yılda 4 Sayı
- Başlangıç: 1988
- Yayıncı: Gazi Üniversitesi, Fen Bilimleri Enstitüsü