Hong Thien VAN, Thi Ngan Ha BUI, Yen Hoa MAI, Minh Nhat Hao NGUYEN, Tan Viet PHAM, Van Son LE, Van Dat DOAN, Le Pham Tan QUOC

Chemical Composition and Antibacterial Activities of Ethanol Extract of Geodorum attenuatum Griff. (Orchidaceae)

Geodorum attenuatum has been found in Thailand, China, India and Vietnam, however, the phytochemical composition and antimicrobial activity of G. attenuatum have not been studied yet. In this study, the phytochemical composition of ethanol extract of G. attenuatum tuber was determined using liquid chromatography-mass spectrometry (LC-MS) and evaluated its antibacterial activity of ethanol extract via disk diffusion test. 9 compounds in ethanol extract of G. attenuatum tuber were identified, such as disaccharide, oxoheneicosanoic acid, shikimic acid, chlorogenic acid, 1-Oleoyl-2-acetyl-glycerol, azafrin, 5,6,7-trihydroxyfavone, 2,3-isopropylidene-1-oleoyl-glycerol and trans-docosanoyl ferulate. Moreover, it’s been proved that the ethanol extract of G. attenuatum tuber could inhibit the growth of B. cereus, E. coli, P. aeruginosa, S. enteritidis, S. typhimurium, and S. aureus.

Keywords:

Antibacterial activity, G. attenuatum, chemical composition, Ethanol extract, LC-MS,

PDF

___

Chen, X., Phillip, J.C., Stephan, W.G., “Geodorum”. Flora of China, 25: 258–260, (2009).
Keerthiga, M., Anand, S.P., “Antifungal Activity of Geodorum densiflorum (Lam.) Schltr. against Pathogenic Fungi”. Original Article, 2: 1456–1461, (2014).
Keerthiga.M., Anand, S.P., “Anti-infective and anti-biofilm activity of Geodorum densiflorum (Lam.) Schltr. against Methicillin resistant and sensitive Staphylococcus aureus”. Advances in Applied Science Research,6: 43–46, (2015).
Averyanov, L.V., Averyanova, A.L., “Updated checklist of the Orchids of Vietnam”, Vietnam National University Publishing House, Hanoi, Vietnam, (2003).
Huyen, D.D., “A New Record for the Flora of Vietnam: Geodorum citrinum Jacks. (Orchidaceae)”. Gardens’ Bulletin Singapore,61: 45–48, (2009).
Nguyen, T.B., Averyanov L., Dương, D.H., “Orchidaceae. Checklist of the flora of Vietnam”. Agricultural Publishing House, Ha Noi, Vietnam, (2005).
Pham-Hoang, H. Araceae. In: Pham-Hoang H. (ed.), “Cây cỏ Việt Nam: An Illustrated Flora of Vietnam”. Youth Publishing House, Ho Chi Minh City, Vietnam, (2000).
Calcutta J. Nat., “Griffith. Geodorum”. Indian Botanical Society, 5: 358, (1845).
Kumar, P., Rawat G.S. Jalal, J.S., “Geodorum attenuatum Griff. (Orchidaceae)”. A new record from India, Richardiana, 8: 80–87, (2008).
Bui, T.N.H., Mai, Y.H., Nguyen, M.N.H., Nguyen, N.T., Le, V.S., Trinh, N.N., Van, H.T., “New distribution in Vietnam and identification of DNA barcode sequence for Geodorum attenuatum (Orchidaceae)”. Journal of Agricultural and Rural Development, 5: 67–73, (2019)
Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., “Phytochemicals: Extraction, Isolation, and Identification of Bioactive Coumpounds from Plant Extracts”. Plants, 6: 1–23, (2017).
Bauer, A.W., Kirby, W.M., Sherris, J.C., Turck, M., “Antibiotic susceptibility testing by a standardized single disk method”. The American Journal of Clinical Pathology, 45: 493–436, (1996).
Anna, J.B., Izabela, J.M., Anna, P., Emilia, S., Paweł, K., “The chemical composition of the floral extract of Epipogium aphyllum Sw. (orchidaceae): a clue for their pollination biology”, Archives of Biological Science Belgrade, 66: 989–998, (2014).
Mariza, G.R., Aparecida, D. Volker, B. Maria do Carmo E. Amaral, Anita J. Marsaioli., “The Chemistry of Flower Rewards–Oncidium (Orchidaceae)”. Journal of the Brazilian Chemical Society, 11: 600–608, (2000)
Pathrapa, R., Boonchoo, S., Kittisak, L., “Chemical Constituents of Dendrobium williamsoni”. Pharmacognosy Journal, 6: 36–41, (2014).
Genova, J., Zheliaskova, A., Mitov, M.D., “Monosaccharides (fructose, glucose) and disaccharides (sucrose, trehalose) influence the elasticity of SOPC membranes”. Journal of Optoelectronics and Advanced Materials, 9: 427–430, (2007).
Eykman, J.F., “Sur les principes constituants de l'Illicium religiosum”. Recueil des Travaux Chimiques des Pays-Bas, 4:32–54, (1885).
Aldesuquy, H.S., Ibrahim. A.H.A., “The Role of Shikimic Acid in Regulation of Growth, Transpiration, Pigmentation, Photosynthetic Activity and Productivity of Vigna sinensis Plants”. Phyton, 40: 277–292, (2000).
Amalia,M.E., Ramon,J.E., “A Short Overview on the Medicinal Chemistry of (-)-Shikimic Acid”. Mini-Reviews in Medicinal Chemistry, 12: 1443–1454, (2012).
Shigetada, F., Takuya, F., Michio, S., Masanori, I., Yukio, Y., Yoshimitsu, N., Osamu, N., Makoto, M., Morihiro, M., Iichiro, S.,” Increased oxidative stress in obesity and its impact on metabolic syndrome”. Journal of Clinical Investigation, 114: 1752–1761, (2004).
Kassi, E., Pervanidou, P., Kaltsas, G., Chrousos, G. Metabolic syndrome., “Definitions and controversies”. BMC Medicine, 5: 35-48, (2011).
Yanga, S., Chou, G., Li, Q., “Cardioprotective role of azafrin in against myocardial injury in rats via activation of the Nrf2-ARE pathway”. Phytomedicine, 47: 12–22, (2018).
Wang, J., Wang, L., Lou, G.H., Zeng, H.R., Hu, J., Huang, Q.W., Peng, W., Yang, X.B., “Coptidis Rhizoma: a comprehensive review of its traditional uses, botany, phytochemistry, pharmacology and toxicology”. Pharmaceutical Biology, 57: 193–225, (2019).
Fu, Y. “Baicalein, A novel anti-diabetic compound”. Master's degree, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, America, (2012).
Christina, H., Dana, A., Lutz, F. Olof Radmark, Dieter Steinhilber., Oliver Werz., “1-Oleoyl-2-acetylglycerol Stimulates 5-Lipoxygenase Activity via a Putative (Phospho) lipid Binding Site within the N-terminal C2-like Domain”. Journal of Biological Chemistry, 280: 26913–26921, (2005).
Mashima, R., Okuyama, T., “The role of lipoxygenases in pathophysiology; new insights and future perspectives”. Redox Biology, 6: 297–310, (2015).
Akter, S., Imam, M., Akter, T., “Antimicrobial activity of different extracts of Geodorum densiflorum (Lam) Schltr. Pseudobulb”. Stamford Journal of Pharmaceutical Sciences, 3: 47–48, (2010).