Effect of gallic acid on liver injury during obstructive cholestasis after bile duct ligation in rat

Aim: To investigate the hepatoprotective, anti-inflammatory and antioxidant effects of gallic acid (GA) against obstructive cholestasis (OC) -induced liver damage in rats. Methods: Thirty female Sprague-Dawley rats were divided into three groups. Group 1 (n=10) was the sham-operated group. In group 2 and group 3, hepatoduodenal ligament dissection was performed after laparotomy. Once the common bile duct was made apparent, it was ligated with 4/0 silk surgical suture and cut between both sutures. Group 2 (n=10) was the control group. Group 3 (n=10) was the GA group. GA (50 mg/kg) was administered by oral gavage daily for 10 days. At the end of the experiment on day 10, the rats were anesthetized. Fibrosis, inflammation, ductal proliferation and necrosis were evaluated histopathologically. Serum levels of AST, ALT, TBIL, DBIL, LDH and GGT levels were determined. In the serum and liver, TAS, TOS, MDA, TNF-α, IL-1, IL-6, and IL 10 levels were evaluated. Results: When group 2 and group 3 were compared histopathologically, fibrosis and inflammation were significantly lower in group 3. In group 3, all LFTs (except DBIL), liver and serum IL-6, IL-1, TOS, MDA, and TNF-α levels were significantly lower than group 2, whereas IL-10 and TAS values were increased. Conclusion: Findings of this research indicate that GA may be effective against OC-induced liver damage in a rat model. We presume that the beneficial effects of GA are closely associated with its antioxidant and anti-inflammatory activities. Therefore, we think that using GA can save us time before resorting to the surgical method.

Kaynakça

[1] Gondal B, Aronsohn A. A Systematic Approach to Patients with Jaundice.Semin Intervent Radiol. 2016;33(4):253-58. Review.

[2] Emerick KM, Whitington PF. Molecular basis of neonatal cholestasis. Pediatr Clin North Am. 2002;49(1):221-35.

[3] Li Y, Tang R, Leung PSC, et al. Bile acids and intestinal microbiota in autoimmune cholestatic liver diseases. Autoimmun Rev. 2017;16(9):885-96.

[4] Pauli-Magnus C, Meier PJ. Hepatocellular transporters and cholestasis. J Clin Gastroenterol. 2005;39(4 Suppl 2):S103-10.

[5] Navaneethan U, Jayanthi V, Mohan P. Pathogenesis of cholangitis in obstructive jaundice-revisited. Minerva Gastroenterol Dietol. 2011;57(1):97-104.

[6] Trauner M, Wagner M, Fickert P, et al. Molecular regulation of hepatobiliary transport systems: clinical implications for understanding and treating cholestasis. J Clin Gastroenterol. 2005;39(4 Suppl 2):S111-24.

[7] Addley J, Mitchell RM. Advances in the investigation of obstructive jaundice. Curr Gastroenterol Rep. 2012;14(6):511-19.

[8] Zhu L, Wang L, Cao F, et al. Modulation of transport and metabolism of bile acids and bilirubin by chlorogenic acid against hepatotoxicity and cholestasis in bile duct ligation rats: involvement of SIRT1- mediated deacetylation of FXR and PGC-1α. J Hepatobiliary Pancreat Sci. 2018;25(3):195-205.

[9] Eslami AC, Pasanphan W, Wagner BA, et al. Free radicals produced by the oxidation of gallic acid: An electron paramagnetic resonance study. Chem Cent J. 2010;4:15.

[10] Chen Y, Zhou G, Ma B, et al. Active Constituent in the Ethyl Acetate Extract Fraction of Terminalia bellirica Fruit Exhibits Antioxidation, Antifibrosis, and Proapoptosis Capabilities In Vitro. Oxid Med Cell Longev. 2019;2019:5176090.

[11] Zhou ZH, Liu MZ, Wang MH, et al. A new ellagic acid derivative from Polygonum runcinatum. Nat Prod Res. 2015;29(9):795- 99.

[12] Nabavi SF, Habtemariam S, Jafari M,et al. Protective role of gallic acid on sodium fluoride induced oxidative stress in rat brain. Bull Environ Contam Toxicol. 2012;89(1):73-77.

[13] Chao WW, Chen SJ, Peng HC, et al. Antioxidant Activity of Graptopetalum paraguayense E. Walther Leaf Extract Counteracts Oxidative Stress Induced by Ethanol and Carbon Tetrachloride Co- Induced Hepatotoxicity in Rats. Antioxidants (Basel). 2019;8(8). pii: E251.

[14] Kim YJ. Antimelanogenic and antioxidant properties of gallic acid. Biol Pharm Bull. 2007;30(6):1052-55.

[15] Priscilla DH, Prince PS. Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. Chem Biol Interact. 2009;179(2-3):118-24.

[16] Criado M, Flores O, Ortíz MC, et al. Elevated glomerular and blood mononuclear lymphocyte nitric oxide production in rats with chronic bile duct ligation: role of inducible nitric oxide synthase activation. Hepatology. 1997;26(2):268-76.

[17] Yu Z, Song F, Jin YC, et al. Comparative Pharmacokinetics of Gallic Acid After Oral Administration of Gallic Acid Monohydrate in Normal and Isoproterenol-Induced Myocardial Infarcted Rats. Front Pharmacol. 20186;9:328.

[18] Ishak K, Baptista A, Bianchi L, et al. Histological grading and staging of chronic hepatitis. J Hepatol. 1995;22(6):696-99.

[19] Ara C, Kirimlioglu H, Karabulut AB, et al. Protective effect of resveratrol against oxidative stress in cholestasis. J Surg Res. 2005;127(2):112-17.

[20] Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004;37(2):112-19.

[21] Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 1990;186:407-21.

[22] Maillette de Buy Wenniger L, Beuers U. Bile salts and cholestasis. Dig Liver Dis. 2010;42(6):409-18.

[23] Yang H, Ramani K, Xia M, et al. Dysregulation of glutathione synthesis during cholestasis in mice: molecular mechanisms and therapeutic implications. Hepatology. 2009;49(6):1982-91.

[24] Georgiev P, Jochum W, Heinrich S, et al. Characterization of time-related changes after experimental bile duct ligation. Br J Surg. 2008;95(5):646-56.

[25] Woolbright BL, Jaeschke H. Novel insight into mechanisms of cholestatic liver injury. World J Gastroenterol. 2012;18(36):4985- 93.

[26] He H, Mennone A, Boyer JL, et al. Combination of retinoic acid and ursodeoxycholic acid attenuates liver injury in bile duct-ligated rats and human hepatic cells. Hepatology. 2011;53(2):548-57.

[27] Zong Y, Zhang M, Li S, et al. Effects of Ethyl Pyruvate on Bile Duct Ligation- Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats. Gastroenterol Res Pract. 2019;2019:2969802.

[28] Feldman M, Friedman LS, Brandt LJ. Sleisenger and Fordtran's Gastrointestinal and Liver Disease (9th Eds): Philadelphia, PA: Saunders Elsevier, 2010.

[29] Xie Y, Guo C, Liu Y, et al. Dexmedetomidine activates the PI3K/Akt pathway to inhibit hepatocyte apoptosis in rats with obstructive jaundice. Exp Ther Med. 2019;18(6):4461-66.

[30] Inkielewicz-Stępniak I. Impact of fluoxetine on liver damage in rats. Pharmacol Rep. 2011;63(2):441-47.

[31] Karimi-Khouzani O, Heidarian E, Amini SA. Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats. Pharmacol Rep. 2017;69(4):830-35.

[32] Cao F, Liu P, Zhang X, et al. Shenqi Fuzheng Injection impairs bile duct ligationinduced cholestatic liver injury in vivo. Biosci Rep. 2019;39(1). pii: BSR20180787.

[33] Demirbilek S, Akin M, Gürünlüoğlu K, et al. The NF-kappa B inhibitors attenuate hepatic injury in bile duct ligated rats. Pediatr Surg Int. 2006;22(8):655-63.

[34] Okur MH , Zeytun H, Basuguy E, et al. Protective effects of cordycepin on the histopathological changes and oxidative stress induced by hepatic ischemia/reperfusion in rats. Exp Biomed Res. 2018; 1(1):17-24.

[35] Aycicek A, Ipek A. Maternal active or passive smoking causes oxidative stress in cord blood. Eur J Pediatr. 2008;167(1):81- 85.

[36] Akcilar R, Akcilar A, Savran B, et al. Effects of ukrain in rats with intestinal ischemia and reperfusion. J Surg Res. 2015;195(1):67-73.

[37] Coban S, Yildiz F, Terzi A, et al. The effects of Nigella sativa on bile duct ligation induced-liver injury in rats. Cell Biochem Funct. 2010;28(1):83-88.

[38] Zeren S, Bayhan Z, Koçak C, et al. Antioxidant Effect of Ukrain Versus NAcetylcysteine Against Acute Biliary Pancreatitis in An Experimental Rat Model. J Invest Surg. 2017;30(2):116-24.

[39] Nishimoto N, Kishimoto T. Interleukin 6: from bench to bedside. Nat Clin Pract Rheumatol. 2006;2(11):619-26.

[40] Stylianou E, Saklatvala J. Interleukin-1. Int J Biochem Cell Biol. 1998;30(10):1075-79.

[41] Tracey KJ. The inflammatory reflex. Nature. 2002;420(6917):853-59.

[42] Pan PH, Lin SY, Wang YY, et al. Protective effects of rutin on liver injury induced by biliary obstruction in rats. Free Radic Biol Med. 2014;73:106-16.

[43] Cassatella MA, Meda L, Bonora S, et al. Interleukin 10 (IL-10) inhibits the release of proinflammatory cytokines from human polymorphonuclear leukocytes. Evidence for an autocrine role of tumor necrosis factor and IL-1 beta in mediating the production of IL-8 triggered by lipopolysaccharide. J Exp Med. 1993;178(6):2207-11.

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