Çörek otu yağının sıçan karaciğer gelişimine etkisi
Çörek otu ve çörek otu yağı halk arasında sıklıkla kullanılmaktadır. Fakat gebelerin kullanımında yavruya toksik etkisi bilinmemektedir. Biz bu çalışmada çörek otu yağının sıçan fetüslerinde karaciğer gelişimine etkisini araştırmayı amaçladık. Çalışmamızda 4 tanesi kontrol, 6 tanesi deney grubu olmak üzere 10 adet dişi sıçan kullanıldı. Sıçanlar gebe bırakıldıktan sonra kontrol grubuna gebelik süresince 2.5 ml/kg/gün serum fizyolojik, deney grubuna ise 2.5 ml/kg/gün çörek otu yağı orogastrik yolla verildi. Gebelerin doğumlarını takiben yavru sıçanlar tartılıp servikal dislokasyonla sakrifiye edildi. Yavru sıçanlardan alınan karaciğerler tespit ve doku takibi işlemlerinden geçirildi. Karaciğer bloklarından alınan kesitler hemotoksilen-eozin ve Masson trikrom boyalarıyla boyanıp ışık mikroskobunda incelendi. Gruplar karşılaştırıldıklarında deney grubuna ait yenidoğan karaciğer dokularındaki histopatolojik bulguların, hidropik dejenerasyon dışında, kontrol grubundan istatistiki olarak farklı olmadığı görüldü. Sonuç olarak, gebelikte kullanılan çörek otu yağı, fetüste karaciğer üzerinde önemli bir toksik etki yapmamıştır. Anahtar kelimeler: Çörek otu yağı; karaciğer; sıçan; yenidoğan Abstract
Effects of Nigella sativa oil on rat liver development
Nigella sativa and its oil are frequently consumed among the people. However, its toxic effect on fetus is not known when used during pregnancy. In this study, we aimed to investigate the effect of Nigella sativa oil on the liver development of rat fetus. Ten female rats were used in this study; four of them consist of control group and six of them composed of the experimental group. When the pregnancy was started, 2.5 ml/kg/day saline or 2.5 ml/kg/day Nigella sativa oil were administered by oral orogastric route for control and experimental groups, respectively. After deliveries, newborn rats were weighed and sacrificed by cervical dislocation. Livers, obtained from newborn rats, were fixed with formalin and embedded with paraffin wax to prepare blocks. Sections obtained from liver blocks were stained with hematoxylin-eosin and Masson trichrome, and examined by light microscope. There were no statistically significant differences between groups in histopathological findings of liver tissues of newborns, except hydrophic degeneration, when the groups were compared. In conclusion, using Nigella sativa oil during pregnancy do not produce toxic effects on fetus liver.
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- 1. Salem ML. Immunomodulatory and immunotherapeutic
properties of the Nigella sativa L. seed. Int Immunopharmacol
2005;5(13-14):1749-70
- 2. Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique
NA, et al. A review on therapeutic potential of Nigella sativa: A
miracle herb. Asian Pac J Trop Biomed 2013;3(5):337-52
- 3. Salem EM, Yar T, Bamosa AO, Al-Quorain A, Yasawy MI,
Alsulaiman RM, et al. Comparative study of Nigella sativa and
triple therapy in eradication of Helicobacter pylori in patients
with
non-ulcer
dyspepsia
- Saudi
J
Gastroenterol
2010;16(3):207-14
- 4. Kanter M, Coşkun Ö, Budancamanak M. Hepatoprotective
effects of Nigella sativa L and Urtica dioica L on lipid
peroxidation, antioxidant enzyme systems and liver enzymes in
carbon tetrachloride-treated rats. World J Gastroenterol
2005;11(42):6684-8
- 5. Baydar H. Tıbbi, aromatik ve keyf bitkileri bilim ve teknolojisi
- Süleyman Demirel Üniversitesi, Ziraat Fakültesi, Yayın No:51,
Isparta, 2005;157-9
- 6. Bita A, Rosu AF, Calina D, Rosu L, Zlatian O, Dindere C, et al
- An alternative treatment for Candida infections with Nigella
sativa extracts. Eur J Hosp Pharm 2012;19(2):162
- 7. Fararh KM, Atoji Y, Shimizu Y, Shiina T, Nikami H, Takewaki
T. Mechanisms of the hypoglycaemic and immunopotentiating
effects of Nigella sativa L. oil in streptozotocin-induced diabetic
hamsters. Res Vet Sci 2004;77(2):123-9
- 8. Abel-Salam BK. Immunomodulatory effects of black seeds and
garlic on alloxan-induced diabetes in albino rat. Allergol
Immunopathol (Madr) 2012;40(6):336-40
- 9. El-Mahmoudy A, Matsuyama H, Borgan MA, Shimizu Y, El-
Sayed MG, Minamoto N, et al. Thymoquinone suppresses
expression of inducible nitric oxide synthase in rat
macrophages. Int Immunopharmacol 2002;2(11):1603-11
- 10. Al-Okbi SY, Ammar NM, Soroor KA, Mohammed DA. Impact
of natural oils supplements on disease activity and antioxidant
state of Egyptian patents with rheumatoid arthritis. Medical
Journal of Islamic Academy of Sciences 2000;13(4):161-71
- 11. Alemi M, Sabouni F, Sanjarian F, Haghbeen K, Ansari S. Anti-
inflammatory effect of seeds and callus of Nigella sativa L
- extracts on mix glial cells with regard to their thymoquinone
content. AAPS PharmSciTech 2013;14(1):160-7
- 12. Abdel-Fattah AM, Matsumoto K, Watanabe H. Antinociceptive
effects of Nigella sativa oil and its major component,
thymoquinone, in mice. Eur J Pharmacol 2000;400(1):89-97
- 13. Salem ML, Hossain MS. Protective effect of black seed oil from
Nigella sativa against murine cytomegalovirus infection. Int J
Immunopharmacol 2000;22(9):729-40
- 14. Badary OA, Abdel-Naim AB, Abdel-Wahab MH, Hamada
FMA. The influence of thymoquinone on doxorubicin-induced
hyperlipidemic
nephropathy
in
rats
- Toxicology
2000;143(3):219-26
- 15. Hosseınzadeh H, Parvardeh S. Anticonvulsant effects of
thymoquinone, the major constituent of Nigella sativa seeds, in
mice. Phytomedicine 2004;11(1):56-64
- 16. Ezz HS, Khadrawy YA, Noor NA. The neuroprotective effect of
curcumin and Nigella sativa oil against oxidative stress in the
pilocarpine model of epilepsy: a comparison with valproate
- Neurochem Res 2011;36(11):2195-204
- 17. Daba MH, Abdel-Rahman MS. Hepatoprotective activity of
thymoquinone in isolated rat hepatocytes. Toxicol Lett
1998;95(1):23-9
- 18. Swamy SM, Tan BK. Cytotoxic and immunopotentiating effects
of ethanolic extract of Nigella sativa L. seeds. J
Ethnopharmacol 2000;70(1):1-7
- 19. Medenica R, Mukerjee S, Huschart T, Koffskey J, Corbit W
- Nigella sativa plant extract increases number and activity of
immune component cell in humans. Exp Hematol
1993;21(8):1186
- 20. Bourgou S, Pichette A, Marzouk B, Legault J. Antioxidant, anti-
inflammatory, anticancer and antibacterial activities of extracts
from Nigella sativa (Black Cumin) plant parts. J Food Biochem
2012; 36(5):539-46
- 21. El-Fatatry HM. Isolation and structure assignment of an
antimicrobial principle from the volatile oil of Nigella sativa L
- seeds. Pharmazie 1975;30(2):109-11
- 22. Rathee PS, Mishra SH, Kaushal R. Antimicrobial activity
essential oil, fixed oil and unsaponifiable matter of Nigella
sativa L. Indian J Pharma Sci 1982;44(1):8-10
- 23. Akhtar MS, Riffat S. Field trial of Saussurealappa roots against
nematodes and Nigella sativa seeds against cestodes in children
- J Pak Med Assoc 1991;41(8):185-7
- 24. Randhawa MA, Al-Ghamdi MS. A review of the pharmaco-
therapeutic effects of Nigella sativa. Pakistan J Med Res 2002;
41(2):77-83
- 25. Al-Ghamdi MS. The anti-inflammatory, analgesic and
antipyretic activity of Nigella sativa. J Ethnopharmacol
2001;76(1):45-8
- 26. Ghosheh OA, Houdi AA, Crooks PA. High performance liquid
chromatographic analysis of the pharmacologically active
quinones and related compounds in the oil of the black seed
(Nigella sativa L.). J Pharm Biomed Anal 1999;19(5):757-62
- 27. Türkdoğan MK, Özbek H, Yener Z, Tuncer I, Uygan I, Ceylan
E. The role of Urtica dioica and Nigella sativa in the prevention
of carbon tetrachloride-induced hepatotoxicity in rats. Phytother
Res 2003;17(8):942-6
- 28. Knodell RG, Ishak KG, Black WC, Chen TS, Craig R,
Kaplowitz N, et al. Formulation and application of a numerical
scoring system for assessing histological activity in
asymptomatic
chronic
active
hepatitis
- Hepatology
1981;1(5):431-5
- 29. Jick H, Walker AM, Porter J. Drug-induced liver disease. J Clin
Pharmacol 1981;21(8-9):359-64
- 30. Lee MG, Hanchard B, Williams NP. Drug-induced acute liver
disease. Postgrad Med J 1989;65(764):367-70
- 31. Salomi NJ, Nair SC, Jayawardhanan KK, Varghese CD,
Panikkar KR. Antitumor principles from Nigella sativa seeds
- Cancer Lett 1992;63(1):41-6
- 32. Türkdoğan MK, Ağaoğlu Z, Yener Z, Sekeroğlu R, Akkan HA,
Avci ME. The role of antioxidant vitamins (C and E), selenium
and Nigella sativa in the prevention of liver fibrosis and
cirrhosis in rabbits: new hopes. Dtsch Tierarztl Wochenschr
2001;108(2):71-3
- 33. Nagi MN, Alam K, Badary OA, Al-Shabanah OA, Al-Sawaf
HA, Al-Bekairi AM. Thymoquinone protects against carbon
tetrachloride hepatotoxicity in mice via an antioxidant
mechanism. Biochem Mol Biol Int 1999;47(1):153-9
- 34. Meral I, Kanter M. Effects of Nigella sativa L. and Urtica
dioica L. on selected mineral status and hematological values in
CCl4-treated rats. Biol Trace Elem Res 2003;96(1-3):263-70
- 35. Kumar V, Cotran R. Robbins Basic Patology. Çevikbaş U
(Editör). İstanbul: Nobel kitabevi, 2003:24,82