Sheu Kadiri RAHAMON, Ganiyu Olatunbosun ARİNOLA

Immunoglobulin classes and acute phase proteins ın the breast milk and plasma of nigerian hıv-ınfected lactating mothers

İnsan anne sütü ve enfeksiyon arasındaki ilişki özellikle gelişmekte olan ülkelerde daha fazla araştırmaya ihtiyaç duymaktadır. Bu çalışma HIV enfeksiyonunun HIV enfekte emziren annelerin plazma ve anne sütündeki immünolojik faktörler üzerine etkisini araştırmayı amaçlamıştır. Immünoglobülin G,A,M ve E, ceruloplasmin (CLP), kompleman faktör IIIc (C3c), α1-antitripsin (A1AT), ve transfer faktör (TRF) 20 asemptomatik HIV enfekte emziren kadın (HIA) ve 30 yaş-uyumlu HIV (-)(seronegatif) emziren anne (HFA)de Enzyme Linked Immunosorbent Assay ve Single Radial Immunodifüzyon teknikleri kullanılarak değerlendirildi. IgMnin Ortalama plazma düzeyi HIAda HFA ile karşılaştırıldığında belirgin olarak yükselmiş, ortalama plazma IgE düzeyi belirgin olarak düşük olarak tespit edildi. IgA, IgM, CLP, C3c ve TRFnin ortalama anne sütü değerleri HIAlerde HFA annelere kıyasla belirgin olarak artmıştı. HIAlerin plazma ve anne sütündeki IgE arasında belirgin pozitif korelasyon ortaya çıkmıştı ve plazma IgG HFAlerin süt IgGsi ile belirgin pozitif korelasyona sahipti. Şiddetli hipergamaglobulinemi Nijeryalı HIAlerin bir bulgusu olmayabilir ve artmış CLP ve TRF düzeyleri sırasıyla HIAlerin sütünde düşük Cu ve Fe düzeylerini göstermektedir.

Nijerya da hıv le enfekte emziren kadınların plazma ve sütünde immunoglobulin sınıfları ve akut faz proteinleri

Relationship between infections and human breast milk still requires more research especially, in developing countries. This study aims to evaluate the effects of HIV infection on immunologic factors in the breast milk and plasma of HIV infected lactating mothers. Plasma and breast milk concentrations of immunoglobulins G, A, M and E, caeruloplasmin (CLP), complement factor IIIc (C3c), α1-antitrypsin (A1AT), and transferring (TRF) were determined in 20 asymptomatic HIV-infected lactating mothers (HIM) and 30 age-matched HIV-free (seronegative) lactating mothers (HFM) using Enzyme Linked Immunosorbent Assay and Single Radial Immunodiffusion technique. The mean plasma level of IgM was significantly elevated in HIM compared with HFM while the mean plasma level of IgE was significantly low in HIM compared with HFM. The mean breast milk levels of IgA, IgM, CLP, C3c and TRF were significantly elevated in HIM compared with HFM. Significant positive correlation existed between IgE in the plasma and breast milk of HIM and plasma IgG had significant positive correlation with the milk IgG in HFM. Severe hypergammaglobulinaemia may not be a feature of Nigerian HIM and increased levels of CLP and TRF indicate low levels of Cu and Fe respectively in the breast milk of HIM.

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