A study on the clinical application of a rapid diagnostic reagent for measles

Kızamık paramiksovirüs ailesine bağlı kızamık virüsünün yolaçtığı ateşli döküntülü bir akut infeksiyondur. Her yıl dünya üzeri- nde yirmi milyondan fazla kişi hastalıktan etkilenmekte ve 242.000 hasta ise ölmektedir. Geleneksel olaral kızamık tanısı gün- lük pratikte klinik semptomlar ile konulmaktadır. Yakın zamanda, birçok hasta tipik klinik özellikler göstermemektedir ve hastalığın laboratuvar doğrulaması kesin gerekli olmaktadır. Yan akım tabanlı immün kramotografi ayıraçları özel eğitim ve beceri gerektirmediğinden Japon genel kliniklerinde hızlı bakım testinde geniş olarak kullanılmaktdır. Yan akım tabanlı immün kramoto- grafi ile uygulanan bir hızlı tanısal ayıraç geliştirdik. Tanısal ayıraç ve RT-PCR, tanısal ayıraç ve serum IgM antikorları, laboratruvar tanı ve klinik bulgular veya semptomlar arasındaki korelasyon sonuçları araştırıldı. Tanısal ayıraç ve RT-PCR arasındaki korelasyon sonuçları pozitif %45.5, negatif %100 oranında iken tanısal ayıraç ve serum IgM antikorları için pozitiflik %45.5, negatiflik %100 bulundu. Klinik bulgular ile tanı konulan kızamık olguları laboratuvar doğrulaması ile ayrıcı tanı yapılarak ortaya konulmuştur. Sonuç olarak, kızamık için bulunan yan akım tabanlı hızlı tanısal ayıraç genel klinikler için yararlı bulunmuştur.

Kızamık için hızlı tanısal ayıraçın klinik olarak kullanımı

Measles is an acute febrile rashes and infections caused by the measles virus belonging to the paramyxovirus. Each year more than twenty million peoples are affected to measles all over the world and 242,000 patients are leading to death. Traditionally the diagnosis of measles had been based on clinical symptoms in daily clinical practice. In recent years, many clinical cases do not show typical clinical manifestations and laboratory confirmation of the disease is definitely required. Lateral flow-based immuno-chromatography reagents are widely used for rapid point-of-care testing in Japanese general clinics, because it does not require special skills and facilities. We have developed a rapid diagnostic reagent for measles that employs the lateral flow-based immuno-chromatography. The correlation of the results between obtained by the diagnostic reagent and RT-PCR assay, the diag- nostic reagent and detection of serum IgM antibodies, and laboratory diagnosis and clinical findings or symptoms was investigated. The correlation of the results between obtained by the diagnostic reagent and RT-PCR was the positive results that match rate of 45.5%, 100% negative match, correlation of the diagnostic reagent and detection of serum IgM antibodies positive matches to 55.6%, 100% negative match. In what had been diagnosed with measles in clinical findings so far identified with differential diag- nosis of measles cases obtained by laboratory confirmation. It was concluded that the lateral flow-based rapid diagnostic reagent for measles was useful in general clinics.

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