Kore'deki broyler tavuklarda Eimeria tenella ve Eimeriaa cervulina'nın internal transcribed spacer 1, ITS- 1fragmentine dayalı filogenetik analizi ve karşılaştırılması

Amaç: Bu çalışmanın amacı Kore'de kanatlıları infekte eden Eimeria tenella, Eimeria acervulina'nın tespit edilmesini sağlayacak basit bir PZR gerçekleştirmek ve aynı ve komşu türler ile filogenetik ilişkisini belirlemektir.Gereç ve Yöntem: İki kanatlı Eimeria türünün (E. tenella ve E. acervulina) ribosomal RNA genlerinin internal transcribed spacer 1 (ITS-1) bölgelerinin sekans analizleri yapıldı ve sahadan elde edilen örneklerle bunlar arasında filogenetik ilişki analiz edildi.Bulgular: Kore'deki E. tenella ve E. acervulina'ların 273-bp ve 147-bp uzunluktaki sekansları kısmi nükleotid sekansları kullanılarak elde edilen filogramlar ile benzerlik gösterdi. Aynı türler içinde sekanslar E. tenella için %98 ve E. necatrix için %89 homologdur. Aynı türler içinde homoloji E. acervulina için %97, E. maxima için %77 ve E. mitis için %76 olarak belirlendi. Her bir Eimarei türünün ITS bölgesi yeterli türler-arası dizi varyasyonuna sahipti ve kullanılan primer Kore'deki herbir isolattan amplifikasyon yapmak için yeterli idi.Öneri: Bu çalışmada kullanılan moleküler metot benzer Eimeria türleri arasında ayrım yapmak için kullanışlıdır ve kanatlı coccidial enfeksiyonlarının epidemiyolojisi ve teşhisi için faydalı metodlar sunmaktadır.

Comparison and analysis of phylogenetic relationship based on internal transcribed spacer 1, ITS-1 fragment of Eimeria tenella and Eimeria acervulina from broiler chicken in Korea

Aim: The objective of the present study is the simple PCR assay that permits the detection of the Eimeria tenella, Eimeria acervulina that infect domestic fowl in Korea and making phylogenetic relation with in the same and neighboring species.Materials and Methods: The internal transcribed spacer 1 (ITS-1) region of ribosomal RNA genes of two poultry Eimeria species; E. tenella and E. acervulina were sequenced and analyzed the phylogenetic relationship among them from field isolates.Results: About the 273-bp and 147-bp sequence of ITS-1 of E. tenella and E. acervulina in Korea were similar reflected in the phylogram constructed using the partial nucleotide sequences. In case of E. tenella for homology searching the sequences among the same species was 98% and with E. necatrix was 89%. In case of E. acervulina sequence similarity within the same species was 97%, whereas the similarity E. maxima and E. mitis were 77% and 76%, respectively. ITS- 1 region of each Eimeria species had sufficient inter-specific sequence variation and the primer is sufficient to amplify each target Korean isolates.Conclusions: The molecular method examine in the present study appears useful for discriminating among similar Eimeria species providing useful methods for diagnosis and epidemiology of avian coccidial infection.

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