Source identification and entry pathways of banned antibiotics nitrofuran and chloramphenicol in shrimp value chain of Bangladesh

Background: Contamination with residues of banned carcinogenic antibiotic drugs like nitrofuranmetabolites and chloramphenicol (CAP) in frozen shrimp products has become a major concern offood safety for exporting countries. In the present study an approach was taken to identify thesources of such harmful antibiotics in the shrimp value chain of Bangladesh, one of the majorshrimp countries.Material and Methods: Inputs of farms and hatchery systems including feed, feed additives, feedingredients and therapeutic agents were thought to be the sources of contagion. Fish and shrimpfeed, feed ingredients, sediment and water samples of shrimp hatcheries and farms were,therefore, analyzed for 3-Amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), 3-Amino-2oxazolidinone (AOZ), 1-Amino-hydantoin (AHD), Semicarbazide (SEM) and chloramphenicol (CAP) toidentify their source and entry pathways. About 500 g of each 160 feed and feed ingredients werecollected in pyrogens free polyethylene sealed bag and transported to Fish Inspection and QualityControl (FIQC) laboratory, Dhaka, Bangladesh. Whereas 500 mL of each 250 soils and water samplewere collected from hatcheries. Sample preparation and residual metabolites analysis wereconducted using validated liquid chromatography-tandem mass spectrometry (LC-MS/MS)analytical assays on an Waters Alliance 2695 series HPLC and Quattro Micro, API mass spectrometerinstrumentation (Waters Corporation, USA).Results: Among the analyzed 160 feed samples, 38 were found contaminated with CAP and/ornitrofuran metabolites (AMOZ, AOZ, AHD and SEM), where 11, 10, 8, and 9 samples were for shrimpfeed, fish feed, poultry feed and feed ingredients. Imported feed ingredients contained with proteinconcentrates of improper quality were found contaminate with higher level of SEM. Althoughhatcheries were found free from contamination, whereas sediment and water samples of manyshrimp farms were found contaminated with high levels of SEM and CAP.Conclusions: It could be narrated that antibiotic contamination of shrimp products were the use ofantibiotic contaminated feed and feed ingredients in the farms; use of poultry litter to fertilizeponds during mixed culture, because poultry were fed with antibiotic medicated feed from zero dayof feeding and indiscriminate use of insecticides and pesticides at nearby agricultural farms.

Kaynakça

Akiyama DM, Domin WG, Lawrence AL (1992) Penaeid shrimp nutrition. In: Marine Shrimp Culture: Principles and Practices. Elsevier Science Publisher. http://dx.doi.org/10.1111/j.1365-2109.1993.tb00646.x

Akyuz M, Kirbag S (2009) Antimicrobial activity of Pleurotus eryngii var. ferulae grown on various agro-wastes. EurAsian Journal of BioSciences 3: 58-63. http://dx.doi.org/10.5053/ejobios.2009.3.0.8

Alam SMN (2013) Bangladesh in the rapid alert system for food and feed notifications in the period 2000-2012: a review. Veterinarni Medicina 58(8): 399-404.

Anonymous (2003) European Commission Decision 2003/181/EC. Official Journal of European Commission L71: 17-18. Anonymous (2008) Rapid Alert System for Food and Feed (RASFF). European Commission. http://dx.doi.org/10.2772/72905

Anonymous (2013) Food Outlook. Biannual report on global food markets. Food and Agriculture Organization (FAO). Via delle Terme di Caracalla, 00153, Rome. http://www.fao.org/docrep/019/i3473e/i3473e.pdf

Antunes P, Machado J, Peixe L (2006) Illegal use of nitrofurans in food animals: contribution to human salmonellosis. Clinical Microbiology and Infection 12: 1047-1049. http://dx.doi.org/10.1111/j.1469-0691.2006.01539.x

Boyd CE (1995) Bottom soils, sediment and pond aquaculture. Chapman and Hall, New York. http://dx.doi.org/10.1007/978-1-4615-1785-6

Boyd CE (2000) Water quality an introduction. Kluwer Academic Publishers, Springer, New York. http://dx.doi.org/10.1007/978-1-4615-4485-2

Cooper KM, Kennedy DG (2005) Nitrofuran antibiotic metabolites detected at parts per million concentrations in retina of pigs, a new matrix for enhanced monitoring of nitrofuran abuse. Analyst 130: 466-468. http://dx.doi.org/10.1039/B418374F

De Souza SVC, Junqueira RG, Ginn R (2005) Analysis of semicarbazide in baby food by liquid chromatography tandem mass spectrometry (LC-MS/MS). In house method validation. Journal of Chromatography A 1077: 151-158. http://dx.doi.org/10.1016/j.chroma.2005.04.062

Eslamloo K, Akhavan SR, Henry MA (2013) Effects of dietary administration of Bacillus probiotics on the non-specific immune responses of tinfoil barb, Barbonymus schwanenfeldii (Actinopterygii: Cypriniformes: Cyprinidae). Acta Ichthyologica et Piscatoria 43(3): 211-218. http://dx.doi.org/10.3750/AIP2013.43.3.05

Gikas E, Kormali P, Tsipi D, Tsarbopoulos A (1996) Development of a rapid and sensitive SPE-LC-ESI MS/MS method for the determination of chloramphenicol in seafood. Journal of Agricultural and Food Chemistry 52: 1025-1030. http://dx.doi.org/10.1021/jf030485l

Hassan MN, Rahman M, Hossain MB, Hossain MM, Mendes R, Nowsad AAKM (2013) Monitoring the presence of chloramphenicol and nitrofuran metabolites in cultured prawn, shrimp and feed in the Southwest coastal region of Bangladesh. Egyptian Journal of Aquatic Research 39: 51-58. http://dx.doi.org/10.1016/j.ejar.2013.04.004

Hoenicke K, Gatermann R, Hartig L, Mandix M, Otte S (2004) Formation of semicarbazide (SEM) in food by hypochlorite treatment: is SEM a specific marker for nitrofurazone abuse? Food Additives and Contaminants 21: 526-537. http://dx.doi.org/10.1080/02652030410001712484

Hossain MB, Amin SMN, Shamsuddin M, Minar MH (2013) Use of Aqua-chemicals in the hatcheries and fish farms of greater Noakhali, Bangladesh. Asian Journal of Animal and Veterinary Advances 8: 401-408. http://dx.doi.org/10.3923/ajava.2013.401.408

Khong SP, Gremaud E, Richoz J, Delatour T, Guy PA, Stadler RH, Mottier P (2004) Analysis of matrix-bound nitrofuran residues in worldwide-originated honeys by isotope dilution high-performance liquid chromatography-tandem mass spectrometry. Journal of Agricultural and Food Chemistry 52: 5309-5315. http://dx.doi.org/10.1021/jf0401118

McCracken RJ, Blanchflower WJ, Rowan C, Mccoy MA, Kennedy DG (1995) Determination of furazolidone in porcine tissue using thermospray liquid chromatography-mass spectrometry and a study of the pharmacokinetics and stability of its residues. Analyst 120:2347-2351. http://dx.doi.org/10.1039/AN9952002347

McCracken RJ, Kennedy DG (1997) The bioavailability of residues of the furazolidone metabolite 3-amino-2- oxazolidinone in porcine tissues and the effect of cooking upon residue concentrations. Food Additives and Contaminants 14: 507-513. http://dx.doi.org/10.1080/02652039709374558

Nouws JF, Laurensen MJ (1990) Postmortal degradation of furazolidone and furaltadone in edible tissues of calves. Veterinary Quarterly 12: 56-59. http://dx.doi.org/10.1080/01652176.1990.9694243

Pereira AS, Donato JL, Nucci DG (2007) Implications of the use of semicarbazide as a metabolic target of nitrofurazone contamination in coated products. Food Additives and Contaminants 21: 63-69. http://dx.doi.org/10.1080/02652030310001647217

Schwarz S, Kehrenberg C, Walsh TR (2001) Use of antimicrobial agents in veterinary medicine and food animal production. International Journal of Antimicrobial Agents 17: 431-437. http://dx.doi.org/10.1016/S0924-8579(01)00297-7

Uno K, Aoki T, Kleechaya W, Tanasomwang V, Ruangpan L (2006) Pharmacokinetics of oxytetracycline in black tiger shrimp, Penaeus monodon, and the effect of cooking on the residues. Aquaculture 254: 24-31. http://dx.doi.org/10.1016/j.aquaculture.2005.10.031

Wang W, Lin H, Xue C, Khalid J (2004) Elimination of chloramphenicol, sulphamethoxazole and oxytetracycline in shrimp, Penaeus chinensis following medicated feed treatment. Environment International 30: 367- 373. http://dx.doi.org/10.1016/j.envint.2003.08.006

Vroomen LHM, Marcel CJ, Berghmans MC, Bladeren PJV, Groten JP, Wissink CJ, Kuiper HA (1990) In vivo and in vitro metabolic studies of furazolidone: a risk evaluation. Drug Metabolism Reviews 22: 663-676. http://dx.doi.org/doi:10.3109/03602539008991460

Kaynak Göster

  • ISSN: 1307-9867
  • Yayın Aralığı: Yılda 0 Sayı
  • Başlangıç: 2018

115 37