Production of extracellular alkaline protease from Bacillus subtilis RSKK96 with solid state fermentation

Bu çalismada, kati faz fermantasyonu ile Bacillus subtilis RSKK96'dan ekstrasellüler alkalin proteaz üretimi incelenmistir. Enzim üretimi için farkli tarim atiklari substrat olarak kullanilmistir. Tarim atiklari arasinda mercimek kabugu proteaz üretimini (3937.0 U/mg) desteklemistir. Üretim parametleri inkübasyon zamani 120 saat, ekstraksiyon medyumu %1 triton-X100, baslangiç nem içerigi %30, baslangiç pH 9.0 olarak optimize edilmistir. Arabinoz, içeren ortamda yüksek alkalin proteaz üretimi gerçeklesmistir, bunu laktoz, galaktoz ve fruktoz izlemistir. Farkli azot kaynaklari arasinda et özütü alkalin protez üretimini en iyi sekilde indüklemis iken diger azot kaynaklari enzim üretimini baskilamistir. Metal tuzlari arasinda $FeSO _4.7H _2O$ ve $MgSO _4.7H _2O$ proteaz üretimini arttirmistir. En yüksek enzim üretimi (5759.2 U/mg) 1000 mL fermantasyon ortaminda gerçeklesmistir.

Kati faz fermentasyonu ile Bacillus subtilis RSKK96'dan ekstrasellüler alkalin proteaz üretimi

The production of extracellular alkaline protease by producing Bacillus subtilis RSKK96 was studied with solid state fermentation (SSF). Different agro residues as substrate were studied for enzyme production. The highest enzyme production was expressed with lentil husk as units per mass of dry substrate (3937.0 U/mg). Production parameters were optimized as incubation time 120 h, extraction medium Triton-X100 1%, initial moisture content 30%, initial pH 9.0. The high level of alkaline protease was obtained in the medium containing arabinose followed by lactose, galactose, and fructose. Among various nitrogen sources, beef extract was found to be the best inducer of alkaline protease, while other nitrogen sources repressed enzyme production. Among metal salts $FeSO _4.7H _2O$ and $MgSO _4.7H _2O$ was found to increase protease production. The maximum enzyme production (5759.2 U/mg) was observed with lentil husk in 1000 mL of fermentation medium volume.

Kaynakça

Chellappan S, Jasmin C, Basheer SM, Elyas KK, Bhat SG (2006) Chandrasekaran, M. Production, purification and partial characterization of a novel protease from marine Engyodontium album BTMFS10 under solid state fermentation. Process Biochemistry 41: 956-961.

Chutmanop J, Chuichulcherm S, Chisti Y, Srinophakun P (2008) Protease production by Aspergillus oryzae in solid-state fermentation using agroindustrial substrates. Journal of Chemical Technology and Biotechnology 83: 1012-1018.

Cowan D, Daniel R, Morgan M (1985) Thermophilic proteases: properties and potential applications. Trends in Biotechnology 3: 68-72.

El Enshasy H, Abuoul-Enein A, Helmy S, El Azaly Y (2008) Optimization of the industrial production of alkaline protease by Bacillus licheniformis in different production scales. Australian Journal of Basic and Applied Sciences 2(3): 583-593.

Ellaiah P, Srinivasulu B, Adinarayana K (2002) A review on microbial alkaline proteases. Journal of Science and Industrial Research 61: 690-704.

Gangadharan D, Sivaramakrishnan S, Nampoothiri KM, Pandey A (2006) Solid culturing of Bacillus amyloliquefaciens for alpha amylase production. Food Technology and Biotechnology 44(2): 269-274.

Gouda MK (2006) Optimization and purification of alkaline proteases produced by marine Bacillus sp. MIG newly isolated from eastern harbor of Alexandria. Polish Journal of Microbiology 55: 119-126.

Haddar A, Bougatef A, Agrebi R, Sellami-Kamoun A, Nasri M (2009) A novel surfactant-stable alkaline serine-protease from a newly isolated Bacillus mojavensis A21. Purification and characterization. Process Biochemistry 44: 29-35.

Holker U, Lenz J (2005) Solid-state fermentation-are there any biotechnological advantages? Current Opinion Microbiology 8: 301-306.

Johnvesly B, Manjunath BR, Naik GR (2002) Pigeon pea waste as a novel, inexpensive, substrate for production of a thermostable alkaline protease from thermoalkalophilic Bacillus sp. JB-99. Bioresource Technology 82: 61-64.

Joo HS, Kumar CG, Park GC, Kim KT, Paik SR, Chang CS (2002) Optimization of the production of an extra cellular alkaline protease from Bacillus horikoshii. Process Biochemistry 38: 155- 159.

Kumar PPK, Mathivanan V, Karunakaran M, Renganathan S, Sreenivasan RS (2008) Studies on the effects of pH and incubation period on protease production by Bacillus spp. using groundnut cake and wheat bran. Indian Journal of Science Technology 1(4): 1-4.

Leighton TJ, Doi RH, Warren RAJ, Kelln RA (1973) The relationship of serine protease activity to RNA polymerase modification and sporulation in Bacillus subtilis. Journal of Molecular Biology 76: 103-22.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folinphenol reagents. Journal of Biological Chemistry 48: 17-25.

Mala BR, Aparn MT, Mohini SG, Vasanti VD (1998) Molecular and biotechnological aspects of microbial proteases. Microbiology and Molecular Biology Reviews 62(3): 597-635.

Mukherjee AK, Adhikari H, Rai SK (2008) Production of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation (SSF) condition using Imperata cylindrica grass and potato peel as low-cost medium: Characterization and application of enzyme in detergent formulation. Biochemical Engineering Journal 39: 353-361.

Pandey A (2003) Solid-state fermentation. Biochemical Engineering Journal 13: 81-84.

Pandey A, Soccol CR, Nigam P, Brand D, Mohan R, Roussos S (2000) Biotechnological potential of coffee pulp and coffee husk for bioprocesses. Biochemical Engineering Journal 6: 153- 162.

Prakasham RS, Rao CS, Sarma PN (2006) Green gram husk-an inexpensive substrate for alkaline protease production by Bacillus sp. in solid-state fermentation. Bioresource Technology 97: 1449-1454.

Robinson T, Singh D, Nigam P (2001) Solid-state fermentation: a promising microbial technology for secondary metabolite production. Applied Microbiology and Biotechnology 55: 284-289.

Sandhya C, Sumantha A, Szakacs G, Pandey A (2005) Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation. Process Biochemistry 40: 2689-2694.

Singh J, Voohra RM, Sahoo DK (1999) Alkaline protease from a new obligate alkalophilic isolate of Bacillus sphaericus. Biotechnology Letters 21: 921-4.

Uyar F, Baysal Z (2004) Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid state fermentation. Process Biochemistry 39: 1893-1898.

Uyar F, Porsuk I, Kizil G, Yilmaz EI (2011) Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15. EurAsian Journal of BioSciences 5: 1-9.

Wang S, Chen Y, Wang C, Yen Y, Chern M (2005) Purification and characterization of a serine protease extracellularly produced by Aspergillus fumigatus in a shrimp and crab shell powder medium. Enzyme Microbiology and Technology 36: 660-665.

Yang JK, Shih IL, Tzeng YM, Wang SL (2000) Production and purification of protease from a Bacillus subtilis that can deproteinise crustacean wastes. Enzyme Microbiology and Technology 26: 406-413.

Kaynak Göster

  • ISSN: 1307-9867
  • Yayın Aralığı: Yılda 0 Sayı
  • Başlangıç: 2018

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