Phytochemistry and antimicrobial compounds of Hymenocrater calycinus

Hymenocrater cinsi, Lamiaceae familyasina dahil olup, onbir çali türünü barindirir. Bunlarin içinden H. calycinus kuzeydogu Iran'da dogal olarak yetisir. Hymenocrater calycinus'un çiçekli kisimlarindan elde edilen etil asetat ve metanol özütlerinden, kromatografik yöntemlerle dört bilesik izole edildi vespektroskopik data araciligi ile tanimlandilar (MS, 1H- ve 13C-NMR, HMQC, HMBC ve 1H-1H COSY). Çalismamizin ana bilesigi olan rozmarinik asitin etkisi, broth seyreltme metodu kullanilarak, Staphylococcus aureus, Escherichia coli, Candida albicans ve Aspergillus niger'a uygulandi. Izole edilen bilesenler; ß-sitosterol (1), ursolic acid (2), rosmarinic acid (3) ve quercetin-3-O- rutinoside (4) seklinde belirlendi. Bakteri ve mantarlarla yaptigimiz çalisma, rozmarinik asitin Candida albicans (MIC, 250 μg mL-1)'a karsi antifungal bir etkisi oldugunu göstermektedir.

Hymenocrater calycinus özütünün fitokimyası ve antimikrobiyal bileşikleri

The genus, Hymenocrater, belongs to the plant family Lamiaceae which contains eleven shrub species, of which H. calycinus belonges and grows wildly in Northeastern Iran. From the ethyl acetate and methanol extracts of the flowered aerial parts of Hymenocrater calycinus, four compounds were isolated using chromatographic methods and identified by spectroscopic data (MS, 1H- and 13C-NMR, HMQC, HMBC and 1H-1H COSY). The effect of rosmarinic acid, as the main component in our study was applied to Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger by the broth dilution method. Isolated compounds were identified as ß-sitosterol (1), ursolic acid (2), rosmarinic acid (3) and quercetin-3-O- rutinoside (4). The results of our assay against bacteria and fungi show that, rosmarinic acid has an antifungal property against Candida albicans (MIC, 250 μg mL-1).

Kaynakça

Agrawal PK (1989) Carbon-13 NMR of Flavonoids. Elsevier Science, Amsterdam. Firouznia A, Rustaiyan A, Nadimi M, Masoudi S, Bigdeli M (2005). Composition of the essential oil of Hymenocrater calycinus (Boiss.) Benth. from Iran. Journal of Essential Oil Research 7, 527-529.

Goad LJ, Akihisa T (1997) Analysis of sterols. Blackie Academic and Professional, London.

Gohari AR, Hadjiakhoondi A, Sadat-Ebrahimi SE, Saeidnia S, Shafiee A (2005) Cytotoxic triterpenoids from Satureja macrantha C.A. Mey. Daru 13, 177-181.

Gohari AR, Saeidnia S, Matsuo K, Uchiyama N, Yagura T, Ito M, Kiuchi F, Honda G (2003) Flavonoid constituents of Dracocephalum kotschyi growing in Iran and trypanocidal activity. Journal of Natural Medicine 57, 250-252.

Lu Y, Foo LY (1999) Rosmarinic acid derivatives from Salvia officinalis. Phytochemistry 51, 91-94.

Mirza M, Ahmadi L, Tayebikhorrami M. (2001) Volative constituents of Hymenocrater incanus Bunge, an Iranian endemic species. Flavor and Fragrance Journal 16, 239-240.

Momen-Roknabadi N, Gohari AR, Monsef-Esfehani HR, Attar F, Hajiaghaee R, Saeidnia S, Jamalifar H, Kamalinia G, Shahverdi AR (2008) Antifungal and antimicrobial activity of Pentanema divaricatum and its active constituent. Zeitschrift fur Naturforschung 63c, 649-652.

Mozaffarian V (1996). A Dictionary of Iranian Plant Names. Farhang Moaser Publication, Tehran.Petersen M, Simmonds MSJ (2003). Rosmarinic acid. Phytochemistry 62, 121-125.

Rechinger KH (1982). Hymenocrater Fisch & CA Mey. In: Rechinger KH (ed) Flora Iranica,Labiatae, No 150. Akademische Druck-u Verlagsanstalt, Graz (Austria), 239-250.

Zaidi MA, Crow SAJR (2005). Biollogically active traditional medicinal herbs from Balochistan, Pakistan. Journal of Ethnopharmacology 96, 331-334.

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