A novel set of highly polymorphic chloroplast microsatellite and ISSR markers for the biofuel crop Jatropha curcas

Farkli Jatropha curcas aksesyonleri arasindaki genetik iliski ortaya çikarmak amaciyla yeni bir polimaforiz ISSR ve kloroplast mikrosatellit markör seti belirlendi. 10 farkli J. curcas akçesyoru arasindaki toplam 26 fragmani çogaltan toplam 12 polimorfik ISSR primeri kullanildi. Mikrosatellit lokuslarinin türler arasi amplifikasyonu, yeni türler için lokus-spesifik markörleri gelistirilmesi için zaman kazandirici ve ucuz bir yaklasimdir. Üstelik farkli J. curcas aksesyorleri arasindaki genetik varyasyon ve yüksek seviyeli polimorfizmi ortaya çikarmak için, Acorus calamus (Acoraceace) gelistirilen kloroplast mikrosatellit primer çiftleri türler arasi amplifikasyon ve aralarindaki geçerlilik için incelendi. 18 cpSSRs arasindaki, 6 tanesi için (%33,33) iyi amplifikasyon ve polimorfizm belirlendi. Her cpSSR primer çifti basina düsen allellerin sayisi, toplamda 18 varyant içersinde, 2 ile 4 arasinda degisiklik gösterdi. Toplam kloroplast DNA çesitliligi (HT) ve farklilasma (GST) sirasiyla 0,700 ve 0,560 olarak bulundu. Shannon bilgi indeksi (I), ISSR ve cpSSR markörleri kullanilarak J. curcas'in aksesyonlari arasindaki Nei genetik çesitlilik sirayla 0,33±0,28, 0,22±0,19 ve 0,94±0,35, 0,55±0,18 olarak hesaplandi. 1. küme ve 21. küme olarak adlandirilarak iki büyük küme halinde gruplandirilan aksesyonlar arasindaki genetik iliskiler, UPGMA küme analizi kullanilarak ortaya kondu J. curcas'da genetik iliski ve genetik çesitlilik analizi için belirlenen yeni markör setinin iliski önemini belirlemek için sonuçlar tartisildi.

Biyodizel bitkisi Jatropha curcas ıçin yeni yüksek oranda polimorfik kloroplast mikrosatellit seti ve ISSR markörleri

A novel set of polymorphic ISSR and chloroplast microsatellite markers were identified to mark the genetic relationship among different accessions of Jatropha curcas. A total of 12 polymorphic ISSR primers used, amplified a total of 26 fragments among ten different accessions of J. curcas. The cross-species amplification of microsatellite loci is timesaving as well as a cost-effective approach for developing locus specific markers for new species. Further, in an attempt to reveal a high degree of polymorphism and genetic variation among different accessions of J. curcas chloroplast microsatellite primer pairs developed for Acorus calamus (Acoraceace) were examined for cross-species amplification and validation among them. Out of 18 cpSSRs, six (33.33%) were identified as resulting in good amplifications and polymorphism. The number of amplified alleles per cpSSR primer pair ranged from 2 to 4 with the total number of 18 size variants as whole. The 18 size variants at 6 loci among 10 accessions were combined in 4 different haplotypes. The total chloroplast DNA diversity (HT) and the differentiation (GST) were found as 0.700 and 0.560 respectively. Whereas, Shannon's information index (I), Nei's genetic diversity among different accessions of J. curcas using ISSR and cpSSR markers was estimated at 0.33±0.28; 0.22±0.19 and 0.94±0.35; 0.55±0.18 respectively. The genetic relationship among the accessions was established using UPGMA cluster analysis, which were grouped into two major clusters denoted as cluster-I and cluster-II. The implication of the results in devising the importance of a identified novel set of markers for the genetic relationship and genetic diversity analysis in J. curcas is discussed.

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Kaynak Göster

  • ISSN: 1307-9867
  • Yayın Aralığı: Yılda 0 Sayı
  • Başlangıç: 2018

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