Özgür AKGÜL, Özer AKGÜL, Naciye Yakut ÖZGÜR

Aşılı Köpeklerin İdrarlarında Leptospira spp. Varlığının PCR Yöntemi İle Araştırılması

Leptospiroz; gram negatif, sporsuz, kapsülsüz ve aerobik bir bakteri olan Leptospira spp.’nin neden olduğu çoklu organ tutulumları ile seyreden zoonoz bir infeksiyon hastalığıdır. Leptospiroz teşhisi için; klinik/otopsi bulguları, mikroskobi, kültür, hayvan deneyleri, seroloji ve PCR (Polimeraz Zincir Reaksiyonu) yöntemlerinden yararlanılmaktadır. Bu çalışmada, XXXilindeki özel veteriner kliniklerine getirilen leptospiroza karşı aşılanmış köpeklerin idrar örneklerinde Leptospira spp. varlığının hızlı ve duyarlı bir yöntem olduğu bildirilen real-time PCR tekniği ile saptanması ve Leptospira spp. pozitifliği ile idrar yoluna ait semptomlar, yaş ve cinsiyet gibi değişkenlerin istatistiksel ilişkisinin karşılaştırmalı olarak değerlendirilmesi amaçlanmıştır. Çalışmaya, idrar yolu semptomu olan ve olmayan toplam 100 aşılı köpekten (56 dişi, 44 erkek) alınan idrar örnekleri randomize olarak dahil edilmiştir. Leptospira spp. varlığının belirlenmesi amacı ile alınan idrar örneklerinde DNA ekstraksiyonu ve real-time PCR yöntemi uygulanmıştır. Çalışmaya dahil edilen 100 köpeğe ait idrar örneklerinin 7 (%7) tanesi Leptospiraspp. varlığı açısından pozitif bulunmuştur. Saptanan pozitifliklerin gruplara göre düzensiz dağılımı ve olgu sayısının istatistiksel anlamlılığa etki edecek düzeyde yeterli olmayabileceği gibi nedenlerden dolayı; yaş, cinsiyet farklılığı gibi demografik veriler ve semptomu olan veya olmayan köpeklerin PCR pozitiflikleri istatistiksel olarak anlamlı derecede farklı bulunamamıştır. Leptospirozun günümüzdeki öneminin daha açık bir şekilde anlaşılabilmesi, teşhisinde kullanılabilecek en uygun yönteminin belirlenebilmesi, aşılamanın önleyici etkilerinin değerlendirilebilmesi gibi amaçlar ile; daha çok olgu sayısını içeren, prospektif ve tedavi seçeneklerinin de değerlendirildiği geniş araştırmalara gereksinim duyulmaktadır.

Anahtar Kelimeler:

Leptospira spp., Aşı, PCR

Investigation of the Presence of Leptospira spp. PCR in Urine Sample of Vaccinated Dogs

Leptospirosis is a zoonotic infectious disease with multiple organ involvement caused by Leptospira spp., a Gram-negative, non-spore-free, non-capsular and aerobic bacterium. For the diagnosis of leptospirosis; clinical/autopsy findings, microscopy, culture, animal experiments, serology and PCR (Polymerase Chain Reaction) methods are used. In this study, the presence of Leptospira spp. in urine samples of dogs vaccinated against Leptospirosis brought to pri-vate veterinary clinics in Istanbul province was determined by a real-time PCR technique which was reported to be a fast and sensitive method, and a comparative evaluation of Leptospira spp. positivity with urinary tract symptoms, age and gender were compared. Urine samples from 100 vaccinated dogs (56 females, 44 males) with and without urinary tract symptoms were randomly included in the study. In order to determine the presence of Leptospira spp., DNA extraction and real-time PCR were performed in urine samples. Of the 100 dogs included in the study, 7 (7%) were positive for the presence of Leptospira spp.. Due to the irregular distribution of the positives determined by the groups and the number of cases may not be sufficient to affect the statistical significance; demographic data such as age, sex differences and PCR positivity of dogs with or withut symptoms were not found statistically significant. To understand the current importance of leptospirosis more clearly, to determine the most suitable method for diagnosis, to evaluate the preventive effects of vaccination; more studies including prospective and treatment options are needed.

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