This study aimed to determine some antioxidant activities at ribes fruit. Ribes is a strong reservoir of anti oxidants. It hasmany vitamins, proteins, and mineral matters in its composition. Many Ribes types, especially black ribes types with darkerfruits contains polyphenolic compounds in high concentrations, especially due to their antioxidant activities they haveincreasing amounts of anthocyanins and flavonols that are increasingly sought after due to their antioxidant activities. Themost significant anthocyanin found in black ribes are cyanidine -3-glucoside, cyanidine-3-rutinoside, delphinidine-3-glucoside, and delfinidine-3-rutinoside. Natural antioxidants have protecting the body against infections, preventing anemiaby inhibiting decomposition of red blood cells, preventing synthesis of cancerous compounds, diluting the blood byincreasing prostaglandin synthesis (antithrombotic effect), preventing arteriosclerosis, and preventing unwanted effects onmetabolism of active forms with oxidation ability causing liver damage. Its protective effects such as healing effects onsome cancer types are known. Level of Catalase (CAT) was found to be 0.00011 U/mI on average for fresh ribes. Level ofSuperoxide Dismutase (SOD) was found to be 11.6960 U/mI on average for fresh ribes. The level of Reduced Glutathione(GSH) was found to be 0.00011 mmol/dI on average for fresh ribes.In this study ribes was found to display a very strong antioxidant activity. Thus, ribes was demonstrated to be a protectiveantioxidant against oxidative damage against many diseases.
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1. Eyduran SP, Ağaoğlu YS. Ankara (Ayaş) Koşullarında Yetiştirilen Frenk üzümü Çeşitlerinin Bazı Pomolojik ve Bitkisel Özellikleri. Ankara Üniversitesi Ziraat Fakültesi, Tarım Bilimleri Dergisi 2007; 13: 293-298.
2. Meydan, İ., Kizil, G., Demir, H., Toptanci, B. C., and Kizil, M, 2020. In vitro DNA damage, protein oxidation protective activity and antioxidant potentials of almond fruit (Amygdalus trichamygdalus) parts (hull and drupe) using soxhlet ethanol extraction. Adv Trad Med 2020: 20: 571-579. 3. Elliot JG. 1999. Application of antioxidant vitamins in foods and beverages. Food Tech 53: 46-48.
3. Başer CH. 2002. Fonksyonel Gıdalar ve Nutrasötikler, 14. Bitkisel İlaç Hammaddeleri Toplantısı, Bildiriler 29-31 Mayıs 2002, Eskişehir.
4. Brennan RM, 2008. Currants and Gooseberries, Temperate Fruit Crop Breeding, J.F. Hancock (ed.), Chapter 6, 177-196, Scotland, UK.
5. Meydan İ, Demir H. Investigation Of Oxıdatıve Dna Damage Preventıon Actıvıty Of Almond (A. Trichamygdalus) Fruıt Wıth Copper And Wıthout Copper. IOSR Journal of Biotechnology and Biochemistry 2019; 2: 41-44.
6. Wang S. Y and H. Lin, Antioxidant Activity in Fruits and Leaves of Blackberry, Raspberry and Strawberry Varies with Cultivar and Developmental Stage. Journal of Agriculture Food Chemistry 2000; 48: 140-146.
7. Meydan, İ., Investıgatıon of Antıoxıdant, Antımıcrobıal and Dna Damage Preventıon Actıvıty of The Dıplotenıa Turcıca Plant Stabılızed Sılver Nanopartıcles. Fresenius Environmental Bulletin 2021; 30: 1231-1239.
8. Benvenuti S, Pellati F. Melegari M, Bertelli D. Polyphenols, Anthocyanins, Ascorbic Acid and Radical Scavenging Activity of Rubus, Ribes and Aronia. Journal of Food Science 2004; 69: 164-169.
9. Aneta W, O Jan M. Magdalena and W. Joanna, Phenolic Profile, Antioxidant and Antiproliferative Activity of Black and Red Currants (Ribes sp.) From Organic and Conventional Cultivation. International Journal of Food Science and Technology 2013; 48: 715-726.
10. Tabart J. Franck T, Kevers C, Pincemail J, Serteyn D, Defraigne JO, Dommes J.. Antioxidant and Anti–inflammatory Activities of Ribes nigrum Extracts. Food Chemistry 2012; 131: 1116-1122.
11. Pecko L, Takac J, Cvopa J, Smolarz K, Zmarlicki K (1993). Nutrient Contents in Fresh and Processed Currant Fruits, Srict International Symposium on Rubus and Ribes , Acta Horticulturae No : 1993; 352: 205-208.
12. Radovanovic, B., A. Milenkovic-Andjelkovic, A. Radovanovic and M. Andjelkovic, 2015.HPLC Analysis of Phenolic Compounds and Antioxidant Activity of Ribes Fruit Leaves.XX Savetovanje O Biotehnologiji 20: 341-346.
13. Gençaslan, G., 2007. Türkiye’de Tıbbi Amaçla Kullanılan Bazı Bitkilerin Antioksidan Özelliklerinin Taranması (Yüksek Lisans Tezi). Ankara Üniversitesi Sağlık Bilimleri Enstitüsü Eczacılık veFarmokoloji Anabilim Dalı, Ankara, 102 s.
14. Güldaş M, Turantaş F.. Meyvelerin Beslenmedeki Önemi ve Üzümsü Meyvelerin Sağlık Üzerine Etkileri. Gıda. Dünya Yayınları 2000; 12: 97-100.
15. Satue-Gracia MT, Heinonen M, Frankel EN, 1997. Anthocyanins as Antioxidants on Human Low-Density Lipoprotein and Lecithin-Liposome Systems. J.Agric. Food Chem 45: 3362-3367.
16. Wang, H., Cao, G., and Prior, R.L. 1997. Oxygen Radical Absorbing Capacity of Anthocyanins. J. Agric. Food Chem. 45: 304- 309.
17. Heinonen, I.M., Meyer, A.S., and Frankel, E.N., 1998. Antioxidant Activity of Berry Phenolics on Human Low-Density Lipoprotein and Liposome Oxidation. J. Agric. Food Chem. 46: 4107-4112.
18. Beutler E., Duron O., Kelly BM., [1963]. Improved method for the determination of blood glutathione. J Lab Clin Med, 61, 882‐888.
19. Gürgöze S, Şahin T, Durak MH. Memelilerde Ortalama Yaşam Süresi ve Yaşlanma Sürecinde Serbest Radikallerin Rolü. İstanbul Üniv Vet Fak Derg 2007; 33 (1): 43-49.
20. Weisel T, Baum M, Eisenbrand G, Dietrich H, Will F, Stockis JP et al. Ananthocyanin/polyphenolic-rich fruit juice reduces oxidative DNA damage and increases glutathione level in healthy probands. Biotechnology Journal 2006; 1: 388-397.
21. Numanoğlu EN, Çelik AG. Antioksidantların Enzimatik Esmerleşme Üzerine Etkileri. Bilim Armonisi 2018; 1: 3-9.
22. Demir H, Güler A, Demir C, Yaş ve Kuru Üzümde (Vitis vinifera L) Bazı Antioksidan Enzim Aktivitelerinin Belirlenmesi Van Tıp Derg 2020; 27: 150-154.