Ratlarda Amiloid Beta1-42 İle Oluşturulan Deneysel Alzheimer Modelinde Tiyol Disülfit Homeostazisi

Amaç: Bu çalışmanın amacı, amiloid beta 1-42 enjekte edilerek Alzheimer modeli oluşturulan ratlarda, oksidan veantioksidan dengenin yeni bir oksidatif stres belirteci olan dinamik tiyol disülphide homeostazisinin, serum total tiyol,natif tiyol, ve disülfit seviyelerinin araştırılması ve disulfit/total tiyol ve disulfit/natif tiyol oranlarınındeğerlendirilmesidir.Gereç ve Yöntemler: Bu deneysel çalışma 28 rat üzerinde gerçekleştirildi. Denekler her grupta 14 adet olacak şekilde,Alzheimer ve kontrol grubu olarak ikiye ayrıldı. Amiloid beta 1-42, enjeksiyon için bir hafta inkubatörde bekletilerektoksititesi artırıldı. Alzheimer grubunda yer alan hayvanlara bilateral 4 µl amiloid beta 1-42 enjekte edilerek deneyselAlzheimer modeli oluşturuldu. 10 gün beklenildikten sonra tüm hayvanlardan 90/10 mg/kg ksilazine/ketamin anestezisialtında intra kardiyak alınan kan numuneleri ile laboratuvarda TDH parametreleri çalışıldı (nativ tiyol, total tiyol,disülfid, disulfit/total tiyol oranı ve disulfit/natif tiyol oranı) ve bu parametreler gruplar arasında karşılaştırıldı.Bulgular: Çalışma ve kontrol grupları karşılaştırıldığında, serum total tiyol düzeyleri (469,85±30,65 ve 558,00±23,46)ile serum disülfid düzeyleri (182,57±15,74 ve 224,85±11,95), alzheimer modeli oluşturulan grupta kontrol grubuna göreistatistiksel olarak anlamlı bir şekilde azalma görülürken (p0,05).Sonuç: İlgili çalışma, deneysel olarak oluşturulan Alzheimer modellemelerinde serumda dinamik tiyol-disülfidhomeostazını değerlendiren ilk araştırmadır. Çalışmamızın sonuçları TDH’ın deneysel alzheimer modellemelerinde,oksidatif stres mekanizmalarının değerlendirilmesinde ucuz ve kolay yeni bir markerı olabileceğini düşündürmektedir.

Thiol Disulphide Homeostasis in Amyloid Beta 1-42 Induced Experimental Alzheimer's Model

Aim: The aim of the present study was to investigate the dynamic thiol disulphide homeostasis as a novel oxidative stress marker of oxidant and antioxidant balance, and the serum levels of total thiol, native thiol, and disulfide as well as disulphide/total thiol and disulphide/native thiol ratios in rats with Alzheimer's model induced by amyloid beta 1-42 administration. Material and Methods: This experimental study was carried out on 14 rats. The animals were randomly divided into two groups: Alzheimer's group (n = 14) and the control group (n = 14). The amyloid beta 1-42 was kept in the incubator for a week to increase its toxicity. Animals in the experimental groups were bilaterally administered 4 µl amyloid beta 1-42. Following 10 days of waiting, all animals were anesthetized and intra-cardiac blood samples were collected for the analyses of native thiol, total thiol, disulfide, disulphite / total thiol ratio and disulphite / native thiol ratio. Results: We detected that serum total thiol levels were 469.85±30.65 and 558.00±23.46 (p0.05) and serum disulphide levels were 182.57±15.74 and 224.85±11.95 (p

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