Ayşe Nur YILDIRIM, Ferhat ŞİRİNYILDIZ, Recep ÖZMERDİVENLİ

Vazointestinal Peptid ve Naringeninin Rotenon Kaynaklı Deneysel Parkinson Hastalığı Modeli Üzerine Etkileri

Amaç: Bu çalışmanın amacı, çeşitli bilimsel çalışmalar ile etkili oldukları gösterilmiş olan naringenin ve vazointestinal peptidin (VIP) intraperitoneal olarak uygulanmasının ratlarda anti-Parkinson aktivitesi açısından değerlendirilmesidir. Gereç ve Yöntemler: Kırk sekiz adet Wistar albino dişi rat 4 gruba ayrıldı. Kontrol grubuna herhangi bir müdahale yapılmadı, RT grubuna rotenon verilirken, RT+VIP grubuna rotenon ve VIP (25 ng/kg) ve RT+NG grubuna ise rotenone ve naringenin (10 mg/kg) verildi. Tüm tedaviler 14 gün süreyle intraperitoneal yolla uygulandı. Parkinson modelinin davranış üzerindeki etkilerini göstermek için hole and board yöntemi kullanıldı. Deneyin son günü hole and board aparatı ile motor testleri yapıldı. Çalışma tamamlandıktan sonra alınan beyin dokusu örneklerinden biyokimyasal analizler yapıldı. Bulgular: RT grubuyla karşılaştırıldığında, RT+NG (p=0,023) grubunda alfa senkronizasyon düzeyi, hem RT+VIP (p=0,039) hem de RT+NG (p=0,032) gruplarında malondialdehit (MDA) düzeyleri ve RT+VIP (p=0,042) grubunda süperoksit dismutaz (SOD) inhibisyonu anlamlı olarak azalırken, RT+VIP (p=0,042) ve RT+NG (p=0,034) gruplarında ise 8-OHdG seviyeleri anlamlı şekilde arttı. Uygulanan VIP ve naringenin tedavileri ile hem biyokimyasal ve hem de motor aktivitelerinde istatistiksel olarak anlamlı şekilde düzelme saptandı. Sonuç: Elde edilen sonuçlara göre rotenon uygulaması ile Parkinson hastalığının semptomları biyokimyasal olarak oluşturulmuştur. VIP ve naringenin tedavilerinin uygulanması deneysel olarak olumlu etkiler göstermiştir ve Parkinson hastalığı ile mücadelede yardımcı bir tedavi unsuru olarak umut verici olmuştur.

Anahtar Kelimeler:

Parkinson hastalığı, rotenon, sıçanlar, vazointestinal peptit

The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson's Disease

Aim: The aim of this study was to evaluate the intraperitoneal administration of naringenin and vasointestinal peptide (VIP), which are shown effective in various scientific studies, in terms of anti-Parkinsonian activity in rats. Material and Methods: Forty-eight Wistar albino female rats were divided into 4 groups. No intervention was made in the control group, rotenone was given to the RT group, rotenone and VIP (25 ng/kg) to the RT+VIP group, and rotenone and naringenin (10 mg/kg) to the RT+NG group. All treatments were administered intraperitoneally for 14 days. The hole and board method was used to show the effects of the Parkinson's model on behavior. On the last day of the experiment, motor tests were carried out with the hole and board apparatus. After the study was completed, biochemical analyzes were performed from brain tissue samples. Results: In comparison to the RT group, while the alpha-sync level in the RT+NG (p=0.023), malondialdehyde (MDA) levels both in the RT+VIP (p=0.039) and RT+NG (p=0.032), and superoxide dismutase (SOD) inhibition in the RT+VIP (p=0.042) groups decreased significantly, the 8-OHdG levels in the RT+VIP (p=0.042) and RT+NG (p=0.034) groups increased significantly. Statistically significant improvement was found both in biochemical and motor activities with the VIP and naringenin treatments applied. Conclusion: According to the results obtained, the symptoms of Parkinson's disease were formed biochemically by rotenone application. The administration of VIP and naringenin treatments has shown positive effects experimentally and has been promising as an adjunct treatment element in the fight against Parkinson's disease.

Keywords:

Parkinson, rats, rotenone, vasointestinal peptide,

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