Anzel BAHADIR, Recep ERÖZ, Süber DİKİCİ, Anzel BAHADIR

Düzce İli Migren Tanısı Almış Hastalarda ENOS Ekzon (G894T) Polimorfizminin Araştırılması

Amaç: Düzce ili migrenli hastalarda eNOS ekzon (G894T) polimorfizm-hastalık ilişkisininbelirlenmesi amaçlanmıştır.Gereç ve Yöntem: Çalışmaya 51 migrenli hasta ve 51 sağlıklı birey dâhil edilmiştir. BireylerinDNA'sı izole edilerek, eNOS ekzon (G894T) polimorfizmini içeren gen bölgesi PCR yöntemiile çoğaltıldıktan sonra, Mbo I restriksiyon enzim kesim reaksiyonu ile genotip analizlerigerçekleştirilmiştir.Bulgular: Migren ve kontrol grupları arasında yaş (p=0,70), cinsiyet (p=0,529), homosistein(p=0,250) ve trigliserit (p=0,575) değerleri açısından anlamlı bir farklılık bulunmamıştır. Kontrolve migren grubu arasında eNOS ekzon (G894T) polimorfizmi açısından anlamlı bir farklılık(p=0,0001) gözlenirken, migren auralı (MA) ve migren aurasız (MO) grubu arasında anlamlıbir farklılık (p=0,377) saptanmamıştır. Bu polimorfizm ile hem cinsiyet (p=0,760) hem de aileöyküsü arasında (p=0,380) bir farklılık belirlenmemiştir.Sonuç: Düzce ili popülasyonundaki migrenli hastalar ile eNOS ekzon (G894T) polimorfizmiarasında anlamlı bir ilişki (p=0,0001) belirlenmiştir. Gelecek araştırmalarda, migren oluşumunutetikleyici diğer aday genlerin de (MTHFR, ACE vb) yöremizdeki daha fazla hasta serisindeçalışılması öngörülmektedir

Anahtar Kelimeler:

Migren, eNOS ekzon (G894T), polimorfizm

Investigation of ENOS Exon (G894T) Polymorphism in Patients with Migraine in Düzce Province

Objective: Determination of relation between disease and polymorphism has been aimedthrough investigation of eNOS exon (G894T) polymorphism frequency in Duzce province.Materials and Methods: In this study, 51 patients with migraine and 51 healthy individualswere included. The genomic DNA of individuals were isolated and the gene region includingeNOS exon (G894T) polymorphism were amplified by PCR (Polymerase Chain Reaction)method and analysed after digesting by MboI restriction enzyme.Results: There was no difference between migraine and control groups among age (p=0,70),sex (p=0,529) homocysteine (p=0,250) and triglyceride (p=0,575) values. While the eNOS exon(G894T) gene polymorphism between migraine and control groups were significant (p=0,0001),no difference was observed between MA and MO groups for related gene polymorphism(p=0,377). Also, no difference was detected between this polymorphism and both sex (p=0,760)and family history(p=0,380).Conclusion: A significant relationship was determinated between eNOS exon (G894T) genepolymorphism and patients with migraine (p=0,0001). Research on other candidate genes(MTHFR, ACE etc.), having role as trigger in migraine, is being planned to be studied using agreat number of patients

Keywords:

Migraine, eNOS exon (G894T), polymorphism,

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