Ismail ÖZDEMİR, Abdulkadir İSKENDER, Ismet ÖZAYDIN, Aziz Rodan SAROHAN, Gülbin SEZEN YALÇIN, Aslı SOMUNKIRAN, Yavuz DEMİRARAN, Mustafa ALBAYRAK

Can anti–adhesive efficacy of sodium hyaluronate and carboxymethylcellulose membrane be improved further by colchicine and collagenase?

Amaç: Cerrahi sonrası intraperitoneal adezyonların oluşumunu önlemede kollajenaz ve kolşisinin etkinliklerini deneysel hayvan modelinde histopatolojik değerlendirme ile araştırmaktır. Gereç ve Yöntem: Çalışmada 40 adet dişi Wistar Albino cinsi rat kullanıldı. Ratlar, kontrol, seprafilm, kolşisin-seprafilm, kollajenaz-seprafilm olmak üzere dört gruba ayrıldı. Laparotomi ile sağ proksimal uterin horn antimezosalpingeal yüzde bisturi aracılığı ile 0.5 cm’lik bir kesi oluşturuldu. Takiben kesi üstüne deneklerin grubuna uygun olarak 0.5 cm’lik Seprafilm; 2 mg Kolşisin emdirilmiş 0.5 cm’lik Seprafilm; 1μg kollajenaz emdirilmiş 0.5 cm’lik Seprafilm yerleştirilerek batın kapatıldı. Kontrol grubuna herhangi bir ajan uygulanmadı. Ratlar 20 gün sonra sakrifiye edilerek makroskopik ve histopatolojik değerlendirmeleri yapıldı. Histopatolojik inceleme Kanbour-Shakir kriterlerine göre yapıldı. Bulgular: Histopatolojik olarak değerlendirilen beş parametrenin toplamı ile elde edilen histolojik toplam skorun kolşisin-seprafilm grubunda en düşük olduğu görüldü. Kolşisinseprafilm grubunun total histolojik skoru ile kontrol, seprafilm ve kollajenaz-seprafilm gruplarının ortalama histolojik skorları arasında istatistiksel olarak anlamlı ölçüde farklılık bulundu (p

Sodyum hiyaluronat ve karboksimetilselluloz membranın anti- adezif etkinliği kolşisin veya kollojenaz ile artırılabilir mi?

Aim: The aim of this study was to determine the impact of collagenase and colchicine in the prevention of postsurgical adhesion formation in a rat uterine horn model. Materials and Methods: Forty female Wistar albino rats were divided into four equal groups including controls, seprafilm, colchicine-seprafilm and collagenase-seprafilm groups. After laparotomy, a standard 0.5-cm injury was made to the antimesosalpingeal side of the right proximal uterine horn by unipolar cautery. After hemostasis, 0.5 cm seprafilm or colchicine 2 mg-0.5 cm seprafilm or collagenase 1μg – 0.5 cm seprafilm was applied to the site of injury. The abdominal incision was then sutured en bloc and the rats were observed for 20 days. Then, all rats were sacrified and second look laparotomy was performed. Macroscopic adhesion score of each rat was noted and histopathologic scoring was made according to Kanbour-Shakir criteria. Results: All of the five scores of the histologic parameters were lowest in the colchicineseprafilm group. The total histologic score of colchicine-seprafilm group was significantly lower than controls, seprafilm and collagenase-seprafilm groups (p

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