Ahmet NACAR, Yasin SELÇUK, Hamza Malik OKUYAN, Nebihat SEFİL KAPLAN, Erkan DELİGÖNÜL, Emel NACAR

Borik asit uygulamasının sıçan böbrek ve testis dokusunda oluşturduğu hasara karşı Omega-3 yağ asitlerinin koruyucu etkisinin histopatolojik olarak incelenmesi

Amaç: Bu çalışmada borik asitin böbrek ve testis dokuları üzerine toksik etkilerine karşı omega-3 yağ asitlerinin koruyucu etkileri araştırıldı. Yöntemler: Çalışmada 32 adet Wistar albino rat kullanı- larak 4 grup oluşturuldu. Kontrol, Omega-3 (10 gün sü- reyle 400 mg/kg/gün), Borik asit (375 mg/kg/gün, 10 gün), Borik asit+Omega-3. Böbrek ve testis dokuları belirli histopatolojik bulguların yaygınlığına göre puanlandı. Bulgular: Histopatolojik analizde, borik asit testis ve böbrekte anlamlı derecede hasar oluşturdu. En belirgin bulgular böbrekte glomerüllerde büzülme, nekroz, kanama ve tübüler hücrelerde dejenerasyon; testiste ise seminifer tübülde hücre kaybı, hücrelerin bazal laminadan kopması ve epitel hücrelerin dejenerasyonu şeklindeydi. Omega-3 uygulaması bu hasarı belirgin bir biçimde hafifletti. Sonuç: Literatür analizimize göre bu çalışma borik asitin indüklediği böbrek ve testis hasarına karşı omega-3 yağ asitlerinin koruyucu etkilerinin gösterildiği ilk çalışmadır.

Histopathologic investigation of the protective effects of omega-3 fatty acids against boric acid-induced injury in kidney and testis tissue

Objective: In this study, it was aimed to evaluate the effects of boric acid on rat kidney and testis tissues histopathologically. Secondly, the protective effects of omega-3 fatty acid against boric acid-induced renal and testicular toxicity were investigated. Methods: 32 wistar albino rats were divided into 4 groups as follows: Control, Omega-3 (400 mg/kg/day for 10 days), Boric acid (375 mg/kg/day for 10 days) and Boric acid+omega-3 (both drugs same dosage for same day). Kidney and testis tissues were evaluated using a scoring system based on the extent of certain histopathological changes. Results: In histopathological examination, boric acid caused significant degeneration in both testis and kidney tissues. Most evident findings were glomerular shrinkage and necrosis, hemorrhage and tubular cell degeneration in kidneys, and exfoliation of seminiferous tubule cells, detachement of epithelium from basement membrane, decreased cellularity and degeneration in epithelial cells in testis tissues. Omega-3 administration significantly attenuated these changes. Conclusion: To our literature search, this is the first study reporting protective effects of omega-3 fatty acid against boric-acid-induced testicular and renal injury.

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