Burhan ATEŞ, Selim ERDOĞAN, Sema ERDEMOĞLU, Halil İbrahim ULUSOY, Songül ULUSOY, Merve GÖKŞIN KARASLAN

Yenilebilir Bir Bitki Olan Polygonum Cognatum Meissn dan Folik Asit ve Antioksidan Bileşiklerin Basınçlı Sıvı Ekstraksiyon (PLE) sistemi ile Ekstraksiyonu için Optimal Koşulların Belirlenmesi

Madımak (Polygonum cognatum Meissn) bitkisi geleneksel olarak Anadolu’da gıda olarak tüketilen, kozmopolitan bir dağılıma sahip kendiliğinden yetişenbir bitkidir. Bu çalışma temelde bu bitkinin yararlı özelliklerini ortaya çıkarmak adına basınçlı sıvı ekstraksiyon sisteminin optimizasyonunu amaçlar. Optimal koşullar belirlendikten sonra, ekstraksiyon koşullarının antioksidan özellikler üzerine etkisi, toplam fenolik içerik, serbest radikal süpürücü etki ve indirgeme gücü özelinde değerlendirildi. İdeal optimizasyon koşullarının 1 M NaOH ve suyun (1:99) çözegen karışımı ile, 60 dk boyunca , 40 oC sıcalıkta, 1500 psi basınçta folik asit için en ideal olduğu belirlendi. Temelde, antioksidan aktivite için gerekli koşullar folik asit için yapılan optmizasyonlar dikkate alınarak belirlenmiştir. Elde edilen sonuçlara göre antioksidan aktivite seviyelerinin çözücü bileşiminden etkilendiği aynı şekilde diğer optimizasyon parametrelerin de değişkenlik gösterdiği gözlenmiştir.

Optimization Of Extraction Parameters For Folic Acid And Antioxidant Compounds From An Edible Plant (Polygonum Cognatum Meissn) Using Pressurized Liquid Extraction (PLE) System

Madimak (Polygonum cognatum Meissn.) plant is a wild edible plant with a cosmopolitan distribution and also traditionally consumed as food in anatolia. This study aims to optimize the extraction parameters in the pressurized liquid extraction (PLE) system and to search some beneficial properties of this plant. After the optimal extraction conditions was determined, the effect of PLE conditions on antioxidant properties was studied by using plant extracts in terms of total phenolic contents, free radical scavenging capacities and reducing power. A solvent including 1 M NaOH and water (1:99), 60 min of extraction time, 40 oC of extraction temperature and 1500 psi of pressure were optimized and found as the most productive operating conditions for PLE in the folic acid determination. Mainly, optimization of PLE conditions were carried out by considering folic acid amounts. Although antioxidant activity levels differ depending on solvent components, the similar results were obtained such in the other optimization parameters.

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