Ayşe Mine Yılmaz Göler, Leyla Kuru, Elif Çiğdem Altunok, Buse Öncü, Betül Karademir, Ömer Birkan Ağralı

Cytotoxicity and Collagen Expression Effects of Tideglusib Administration on Human Periodontal Cells: An In-Vitro Study

Objective: Tideglusib is a GSK-3 inhibitor activating Wnt/β-catenin signaling pathway which has significant importance in regenerative response.The aim of this study was to evaluate the cytotoxicity and protein expression impacts of Tideglusib on human periodontal cell lines.Methods: Cytotoxicity effect of different concentrations (50nM, 100nM, 200nM) of Tideglusib application on human gingival fibroblast (hGF),periodontal ligament fibroblast (hPDLF), and osteoblast (hOB) cell lines was determined. Type-I and III collagen expressions were evaluatedafter 24-hour application of 50nM Tideglusib.Results: The cytotoxicity of 200nM Tideglusib was higher in hGF and hOB (p0.05). The hGF and hOB treated with 50nM Tideglusib expressed an increased level of Type-I collagen (p0.05). Type-III collagen expressions were similar between thetest and control groups for each cell line (p>0.05).Conclusion: Tideglusib is not cytotoxic at 50nM and 100nM concentrations and may have positive effect on bone regeneration rather thanperiodontal regeneration since it stimulated Type-I collagen production in hGF and hOB cells, but not in hPDLF.

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