Mehmet Berkoz, Metin Yıldırım, Oruç Allahverdiyev, Tahir Kahraman, Evan Abdulkarim Mahmood

Amaç: Juniperus communis L., özellikle Avrasya'da yetişen ve Cupressaceae L. ailesine ait bir çalıdır. Juniperus communis L. meyvelerinin sulu ekstraktının antioksidan ve antidiyabetik aktivitesi, streptozotosin ile indüklenmiş diyabetik albino sıçanlarında potansiyel bir antidiyabetik olarak fayda göstermiştir. Bu çalışma, Juniperus communis L. yağı takviyesinin diyabetik sıçanlarda böbrek fonksiyon bozukluğunu etkili bir şekilde iyileştirip iyileştirmeyeceğini belirlemek amacıyla yapılmıştır. Yöntem: Yirmi sekiz sıçan 4 eşit gruba ayrılmıştır; kontrol grubu, diyabetik grup, Juniperus communis L. yağı (200 mg / kg) ile tedavi edilen grup ve diyabetik + Juniperus communis L. yağı (200 mg / kg) ile tedavi edilen grup. Deney sonunda tüm sıçanlar sakrifiye edildi ve tüm grupların böbrek antioksidan ve lipid peroksidasyon belirteçleri ile serum kreatinin, serum üre, kan üre azotu (BUN) ve serum total protein düzeyleri gibi böbrek fonksiyon parametreleri ölçüldü. Bulgular: Diyabetik grupta HbA1c, serum glukoz, serum üre, serum kreatinin, BUN ve böbrek lipit peroksidasyon düzeyleri kontrol grubuna kıyasla artmış, ancak serum total protein ve antioksidan düzeyleri azalmıştır (p<0,05). Ayrıca Juniperus communis L. yağı ile tedavi edilen diabetik sıçanların HbA1c, serum glukoz, serum üre, serum kreatinin, BUN ve böbrek lipid peroksidasyon düzeylerini azalırken serum total protein ve antioksidan düzeyleri artmıştır (p <0,05). Sonuç: Bu çalışma, diabetes mellitus ve diyabetik nefropati arasında bir bağlantı olduğunu kanıtlamakta ve Juniperus communis L. yağının, böbrek fonksiyon testleri üzerinde iyileşmeye ve oksidatif stres parametrelerinde azalmaya neden olarak böbrek üzerinde koruyucu bir etki sağladığını göstermektedir.

Anahtar Kelimeler:

Juniperus communis L., diyabet, nefropati, antioksidan, lipid peroksidasyonu, oksidatif stres

Can Juniperus communis L. oil improve nephropathy in diabetic rats

Objective: Juniperus communis L. is a shrub belonging to family Cupressaceae L. mainly growth in Eurasia. The antioxidant and antidiabetic activity of aqueous extract of J. communis L. berries indicated benefits as a potent antidiabetic in streptozotocin induced diabetic albino rats. This study was carried out to determine whether J. communis L. oil supplement will effectively manage renal dysfunction in diabetic rats.Methods: Twenty eight rats were divided into 4 equal groups as follows; control group, diabetic group, J. communis L. oil (200 mg/kg) treated group, and diabetic+J. communis L. oil (200 mg/kg) treated group. At the end of the experimental period, all rats were sacrificed and renal function parameters such as kidney antioxidant and lipid peroxidation markers and serum creatinine, serum urea, blood urea nitrogen (BUN), and serum total protein levels were measured in all groups. Results: HbA1c, serum glucose, serum urea, serum creatinine, BUN, and kidney lipid peroxidation levels increased but serum total protein and antioxidant levels decreased in diabetic group comparing with control group (p<0.05). Furthermore, HbA1c, serum glucose, serum urea, serum creatinine, BUN, and kidney lipid peroxidation levels decreased and also, serum total protein and antioxidant levels increased in diabetic group treated with J. communis L. oil comparing with diabetic group (p<0.05). Conclusion: This study has provided direct evidence of a link between diabetes mellitus and diabetic nephropathy and demonstrated that J. communis L. oil provide a protective effect on the kidney as evidenced by an improvement of the renal function tests as well as reduction in oxidative stress parameters.

Keywords:

Juniperus communis L., diabetes, nephropathy, antioxidant, lipid peroxidation, oxidative stress,

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