Büşra Ak, Eylem Atak, Merve Deniz Köse, Oguz Bayraktar

Production of Chlorella sp. in a Designed Photobioreactor

Microalgae are photosynthetic microorganisms that are recently grown to produce biomass for food,pharmaceutical, dye, and bioenergy industries. Microalgae are the carbon source found in crude oil andnatural gas. Over the years, there have been several advances in the design and operation of closedphotobioreactors for microalgal cultures based on new reactor geometries as well as optimized aerationand mixing strategies. Closed photobioreactors ensure heat control, and high productivity througheffective use of high-intensity light and prevent contamination in microalgae production. One of themost important factors that control cell growth in a photobioreactor is light availability. In this study, thecultivation of Chlorella sp., as microalgae were performed in a specially designed photobioreactor forproductivity analysis and pigment capacity analysis. The applied light energy was kept constant whileapplying either continuous or intermittent lighting during the growth of microalgae. The cultivationparameters were tested to find the optimal light mode as the continuous light or 12h light/ 12h darkcycle to maximize pigment amount. In order to determine the pigment amount in the cultivated algaeextraction was done. Then by using UV spectrophotometer amount of chlorophyll a and b weredetermined in the obtained extracts.

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1. Brasier, M. D., Green, O. R., Jephcoat, A. P., Kleppe, A. K., Van Kranendonk, M. J., Lindsay, J. F., ... & Grassineau, N. V. 2002. Questioning the evidence for Earth's oldest fossils. Nature, 416(6876), 76.
2. Converti, A., Casazza, A. A., Ortiz, E. Y., Perego, P., & Del Borghi, M. 2009. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chemical Engineering and Processing: Process Intensification, 48(6), 1146-1151.
3. González‐Fernández, C., Sialve, B., Bernet, N., & Steyer, J. P. 2012. Impact of microalgae characteristics on their conversion to biofuel. Part I: Focus on cultivation and biofuel production. Biofuels, Bioproducts and Biorefining, 6(1), 105-113.
4. Safi, C., Zebib, B., Merah, O., Pontalier, P. Y., & Vaca-Garcia, C. 2014. Morphology, composition, production, processing and applications of Chlorella vulgaris: a review. Renewable and Sustainable Energy Reviews, 35, 265-278.
5. Lee, R., Phycology. Cambridge [England]: Cambridge University Press., 4th ed., 1999, pp.213-217.
6. Abu-Ghosh, S., Fixler, D., Dubinsky, Z. and Iluz, D. 2015. Continuous background light significantly increases flashing-light enhancement of photosynthesis and growth of microalgae. Bioresource Technology, 187, 144-148.
7. Liu, X., Hong, Y., Liu, P. Zhan, J., Yan, R., 2019. Effects of cultivation strategies on the cultivation of Chlorella sp. HQ in photoreactors Frontiers of Environmental Science and Engineering 13: 78 1-11.
8. Carvalho, A., Silva, S., Baptista, J. and Malcata, F. 2010. Light requirements in microalgal photobioreactors: an overview of biophotonic aspects. Applied Microbiology Biotechnology, 89(5), 1275-1288.
9. Chou, H., Su, H., Song, X. 2019. Isolation and characterization of Chlorella sp. mutants with enhanced thermo- and CO2 tolerances for CO2 sequestration and utilization of flue gases. Biotechnology for Biofuels 12, 251.
10. Yılmaz, H. K. 2006. Mikroalg Üretimi için Fotobiyoreaktör Tasarımları. EÜ Su Ürünleri Dergisi, 23(1/2), 327-332.
11. Widayat, J.P. and Wibisono J., 2018. Cultivation of Microalgae Chlorella sp on Fresh Water and Waste Water of Tofu Industry. The 2nd International Conference on Energy, Environmental and Information System (ICENIS 2017), 31, 1-3.
12. Walker, T. L., Purton, S., Becker, D. K., & Collet, C. 2005. Microalgae as bioreactors. Plant cell reports, 24(11), 629-641.
13. Granata, T., 2017. Dependency of Microalgal Production on Biomass and the Relationship to Yield and Bioreactor Scale-up for Biofuels: a Statistical Analysis of 60+ Years of Algal Bioreactor Data. BioEnergy Research, 10 (1), 267–287.
14. Papapolymerou, G., Karayannis, V., Besios, A., Riga, A., Gougoulias, N., Spiliotis, X., 2019. Scaling-up sustainable Chlorella vulgaris microalgal biomass cultivation from laboratory to pilot-plant photobioreactor, towards biofuel. Global NEST Journal, 21, No , 37- 42.
15. Derwenkus, F., Metz, F., Gille, A., Schmid-Staiger, U., Briviba, K., Schliessmann U., Hirth, T., 2019. Pressurized extraction of unsaturated fatty acids and carotenoids from wet Chlorella vulgaris and Phaeodactylum tricornutum biomass using subcritical liquids. Global Change Biology, Bioenergy, 11, 335-344.
16. Kim, J., Lee, J. and Lu, T. 2015. A model for autotrophic growth of Chlorella vulgaris under photolimitation and photoinhibition in cylindrical photobioreactor. Biochemical Engineering Journal, 99, 55- 60.
17. Andersen, R. A. (Ed.). 2005. Algal culturing techniques. Elsevier.
18. ATCC Medium: 616 Medium BG-11 for Blue-green Algae, www.atcc.org
19. Shuler, M. and Kargi, F., Bioprocess engineering. Upper Saddle River, NJ: Prentice Hall 2002.
20. Jeffrey, S.W., Humphrey, G.F. 1975. New spectrophotometric equations for determining chlorophyll a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Journal of Plant Biochemistry Physiology, 167, 191–194.
21. Pottier, L., Pruvost, J., Deremetz, J., Cornet, J., Legrand, J. and Dussap, C. 2005. A fully predictive model for one-dimensional light attenuation by Chlamydomonas reinhardtii in a torus photobioreactor. Biotechnology and Bioengineering, 91(5), 569-582.

Celal Bayar Üniversitesi Fen Bilimleri Dergisi-Cover

ISSN: 1305-130X
Başlangıç: 2005
Yayıncı: Manisa Celal Bayar Üniversitesi Fen Bilimleri Enstitüsü

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