Papain Immobilization on NiFe 2 O 4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin

NiFe 2 O 4 magnetic nanoparticles were solvothermally prepared. NiFe 2 O 4 magnetic nanoparticles werefunctionalized with gallic acid and then papain immobilized on NiFe 2 O 4 functionalized with gallic acid. Theobtained samples were characterized and the activities of free and immobilized papain were studied.Immobilized papain showed higher and more effective activity than free papain. This immobilized papainretained about 75.5% of its initial activity after 8 weeks of storage at 4 °C in sodium phosphate buffer (0.1M, pH 6.5), while the free trypsin protected 30.5% of its initial activity during the same condition.Furthermore, the immobilized papain protected approximately 51% of its initial activity following the timesof ten sequential reuse. Finally, the microwave assisted digestion of bovine serum albumin was carried outfor 15 s with matrix-assisted laser desorption/ionization mass spectrometry by using free and immobilizedpapain.

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