Characterization and in vitro Evolution of Antibacterial Efficacy of Novel Hesperidin Microemulsion

Characterization and in vitro Evolution of Antibacterial Efficacy of Novel Hesperidin Microemulsion

Natural products used in the traditional systems of medicine exhibit a various and promising resource in human health. However, these products are often characterized for their chemical composition as well as mechanisms of action. Considering the bacterial infections play an important role in human diseases, it is necessary to develop new antibacterial herbal products. The aim of this study was to isolation and structural identification of hesperidin from Citrus sinensis L. and to formulate hesperidin loaded to water-in-oil (w/o) microemulsion as an inovative formulation for bactericidal treatment. This present study indicates that hesperidin microemulsion has antibacterial activity against various types of bacteria according to MIC method. All strains were showed logaritmic reduction. These findings indicated that the microemulsion as a drug carrier, especially hesperidin formulation, may be used as an effective antibacterial therapy

___

  • 1. Shakeri A., Amirhossein Sahebkar A., Behjat J., Melissa officinalis L. – A review of its traditional uses, phytochemistry and pharmacology, Journal of Ethnopharmacology, 2016, 188, 204- 228.
  • 2. World Health Organization, WHO, Traditional Medicine Strategy Document 2003. Geneva, Switzerland.
  • 3. Newman D.J. and Cragg G.M. Natural products as sources of new drugs over the last 25 years, Journal of Natural Products, 2007, 70, 3, 461-477.
  • 4. Moteetee A. and Kose L.S., Medicinal plants used in Lesotho for treatment of reproductive and post reproductive problems, Journal of Ethnopharmacology, 2016, 194, 827-849.
  • 5. Omwenga E.O., Hensel A., Shitandi A., Goycoolea F.M. Ethnobotanical survey of traditionally used medicinal plants for infections of skin, gastrointestinal tract, urinary tract and the oral cavity in Borabu sub-county, Nyamira county, Kenya, Journal of Ethnopharmacology, 2015, 24, 508-514.
  • 6. Muhammad A.A., Amir H.K., Muhammad A., Izatullah I. Traditional uses of medicinal plants reported by the indigenous communities and local herbal practitioners of Bajaur Agency, Federally Administrated Tribal Areas, Pakistan, Journal of Ethnopharmacology, 2017, 198, 268-281.
  • 7. İnce İ., Kayalar H., Elgin G., Köksal Ç., Yavaşoğlu K.N.Ü. Antioxidant, Anti-inflammatory Activities and Acute Toxicity of the Polyherbal Formulation: Romix® , Pharmaceutical Biology,2012, 50, 720-726.
  • 8. Mahmoudi S., Khali M., Benkhaled A., Benamirouche K., Baiti I. Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian Ficus carica L. Varieties, Asian Pacific Journal of Tropical Biomedicine, 2016, 6, 239-245.
  • 9. Halfon B. Natural Products, Boğaziçi University Printhouse Lecture notes, 2005, (ISBN: 975.518.251.9). Turkey.
  • 10. Robards, K. and Antolovich, M. Analytical Chemistry of Fruit Bioflavonoids A Review, Analyst, 1997, 122, 11R-34R.
  • 11. Devi P.K., Rajavel T., Nabavi F.S., Setzer N.W., Ahmadi A., Mansouri K., Nabavi M.S. Hesperidin: A promising anticancer agent from nature, Industrial Crops and Products, 2015, 76, 582- 589.
  • 12. Siddiqi A., Hasan K.S., Nafees S., Rashid S., Saidullah B., Sultan S. Chemopreventive efficacy of hesperidin against chemically induced nephrotoxicity and renal carcinogenesis via amelioration of oxidative stress and modulation of multiple molecular pathways, Experimental and Molecular Pathology, 2015, 99, 641-653.
  • 13. Haghmorada D., Mahmoudi B.M., Salehipour Z., Jalayer Z., Brojenie M.A.A., Rastin M., Kokhaei P., Mahmoudi M. Hesperidin ameliorates immunological outcome and reduces neuroinflammation in the mouse model of multiple sclerosis, Journal of Neuroimmunology, 2017, 302, 23-33.
  • 14. Hamdan D., El-Readi M.Z., Tahrani A., Herrmann F., Kaufmann D., Farrag N., El-Shazly A., Wink M. Chemical composition and biological activity of Citrus jambhiri Lush., Food Chemistry, 2011, 127, 394-403.
  • 15. Gündoğdu E., Karasulu Y.H., Köksal Ç. and Karasulu E. The novel oral imatinib microemulsions: physical properties, cytotoxicity activities and improved Caco-2 cell permeability, Journal of Microencapsulation, 2013, 30, 132-142.
  • 16. National Committee for Clinical Laboratory Standards: Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard – Eighth edition, 2007, NCCLS document M7-A6. NCCLS, Wayne, Pennsylvania.
  • 17. Lahmer N., Belboukhari N., Cheriti A. and Sekkoum K. Hesperidin and hesperitin preparation and purification from Citrus sinensis peels. Der Pharma Chemica, 2015, 7, 1-4.
  • 18. Akşit H., Çelik S. M., Şen Ö., Erenler R., Demirtaş İ., Telci İ. and Elmastaş M. Complete isolation and characterization of polar portion of mentha dumetorum water extract. Records of Natural Products, 2014, 8, 277-280.
  • 19. Ghosh P.K., Majithiya R.J., Umrethia M.L., Murthy R.S. Design and development of microemulsion drug delivery system of acyclovir for improvement of oral bioavailability, American Association of Pharmaceutical Scientists Technology, 2006, 7(3), E172-E177.
  • 20. World Health Organization, WHO, Traditional Medicine Strategy Document 2002-2005, 2002. 1346-1351, Geneva, Switzerland.
  • 21. Türkmenoğlu P.F., Kutsal G.Y., Dolgun B.A., Diker Y., Baydar T. Evaluation of herbal product use and possible herbedrug interactions in Turkish elderly, Complementary Therapies in Clinical Practice, 2016, 23, 46-51.
  • 22. Zhang J., Wider B., Shang H., Li X., Ernst E. Quality of herbal medicines: challenges and solutions, Complementary Therapies in Medicine, 2012, 20, 100-106.
  • 23. Aziato L. and Odai N.A.P. Exploring the safety and clinical use of herbal medicine in the contemporary Ghanaian context: A descriptive qualitative study, Journal of Herbal Medicine, 2017, https://doi.org/10.1016/j.hermed.2016.11.002.
  • 24. Yamamoto M., Jokura H., Hashizume K., et al. Hesperidin metabolite hesperetin-7-O-glucuronide, but not hesperetin-3'-Oglucuronide, exerts hypotensive, vasodilatory, and antiinflammatory activity of extract from Castanea sativa leaves, Fitoterapia, 2000, 71, 110-116.
  • 25. Gar A., Garg S., Zaneveld L.J.D., Singla A.K. Chemistry and pharmacology of the citrus bioflavonoid hesperidin, Phytotherapy Research, 2001, 15, 655-669.
  • 26. Wang J.J., Sung K.C., Hu O.Y., Yeh C.H., Fang J.Y. Submicron lipid emulsion as a drug delivery system for nalbuphine and its prodrugs, Journal of Controlled Release, 2006, 115, 140-149.
  • 27. Cui J., Yu B., Zhao Y., Zhu W., Li H., Lou H., Zhai G. Enhancement of oral absorption of curcumin by selfmicroemulsifying drug delivery systems, International Journal of Pharmaceutics, 2009, 371, 148-155.
  • 28. Basile A., Sorbo S., Giordano S., Ricciardi L., Ferrara S., Montesano D., Castaldo C.R., Vuotto M.L., Ferrara L. Antibacterial and allelopathic activity of extract from Castanea sativa leaves, Fitoterapia, 2000, 1, 110-116.
  • 29. Kawaguchi K., Kikuchi S., Hasunuma R., Maruyama H., Yoshikawa T., Kumazawa Y. A citrus flavonoid hesperidin suppresses infection-induced endotoxin shock in mice, Biological and Pharmaceutical Bulletin, 2004, 27, 679-683