Hispidulinin Sağlıklı Hücre Hatlarında Sitotoksik, Apoptotik ve Nekrotik Etkilerinin Değerlendirilmesi

Hispidulin çeşitli farmakolojik özelliklere sahip olmasından dolayı uzun süredir kanser araştırmalarında kullanılmaktadır ancak bu maddenin sağlıklı hücrelerde sitotoksik etkisiyle ilgili sınırlı sayıda çalışma bulunmaktadır. Bu çalışmada ilk defa, hispidulin geniş doz aralığında (0-800 μM) sağlıklı hücre hatları ile denendi ve kulanımının güvenliği MTT ve Akım sitometrisi analizleriyle değerlendirildi. Bu amaçla, İnsan akciğer fibroblastı (MRC-5), Sıçan Primer Akciğer Fibroblastı (RA-6013) ve Sıçan Primer Trakeal Fibroblastı (RA-6217) hücreleri 48 saat boyunca hispidulinle muamele edildi. Hispidulin, düşük dozlarda hücre canlılığı üzerinde önemli etkiler göstermedi ancak özellikle 400 ve 800 μM dozlarında hücrelerin canlılığında istatistiksel anlamda ciddi bir azalmaya neden oldu. Hispidulinin IC50 değeri, MRC-5 hücreleri için 563.63 µM, RA-6013 hücrelerinde 194.30 µM ve RA-6217 hücrelerinde ise 311.98 µM olarak belirlendi. Ayrıca 800 μM hispidulin, tüm sağlıklı hücreler üzerinde güçlü apoptotik ve nekrotik etki gösterdi. Sonuçlarımız, hispidulinin düşük dozlarda (0-200 μM) sağlıklı hücrelerde antisitotoksik nitelikte olduğunu ve gelecekteki kanser ve solunum yolu hastalıkları tedavisi için güvenilir doğal bir bileşik olma potansiyeline sahip olduğunu göstermektedir.

Anahtar Kelimeler:

Hispidulin, MRC-5, RA-6013, RA-6217, Sitotoksisite

Evaluation of Cytotoxic, Apoptotic, and Necrotic Efficiency of Hispidulin in Healthy Cell Lines

Since hispidulin has various pharmacological properties, its use in cancer research has been increasing for a long time, but there are very limited studies on its cytotoxic effect in healthy cells. In this study, for the first time, the safety of hispidulin in a wide dose range (0-800 μM) was evaluated on healthy cell lines by MTT and Flow cytometry assays. For this purpose, Human lung fibroblast (MRC-5), Rat Primary Lung Fibroblast (RA-6013), and Rat Primary Tracheal Fibroblast (RA-6217) cells were treated with hispidulin for 48 hours. Hispidulin did not show significant effects on cell viability at low doses; however, caused a statistically significant reduction in cell viability, especially at doses of 400 and 800 μM. The IC50 value of hispidulin was determined as 563.63 µM for MRC-5 cells, 194.30 µM for RA-6013 cells, and 311.98 µM for RA-6217 cells. Moreover, 800 μM hispidulin showed a strong apoptotic and necrotic effect on all healthy cells. Our results show that hispidulin is anticytotoxic in healthy cells at low doses (0-200 μM) and has the potential to be a safe natural compound for future treatment of cancer and respiratory diseases.

Keywords:

Hispidulin, MRC-5, RA-6013, RA-6217, Cytotoxicity,

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