Serge KAMENİ LEUGOUÉ, Dongmo Nguedıa ARİUS BAULLAND, Tebug THOMAS TUMASANG, Bomba Tatsınkou FRANCİS DESİRE, Félix MEUTCHİEYE, Ngoula FERDİNAND

Spirulina (Arthrospira platensis) Extract Promotes Motility, Microscopic, and Antioxidative Parameters of Ram Semen during Refrigerated Storage

This study investigated the effect of spirulina ethanolic extract (SEE) on the quality of ram semen during low-temperature liquid storage and the relationship between sperm features. Ejaculates were collected from five Djallonké rams, pooled, extended with Tris-egg yolk (TEY) enriched with 0 (control), 20 (SEE20), 40 (SEE40), and 80 µg/mL (SEE80) of SEE to reach the concentration of 200×106 spz/mL, and stored at 4 °C for 72 h. Extended semen samples were assessed for total motility, progressive motility, sperm motion characteristics, viability, membrane integrity, and morphology at 6, 24, 48 and 72 h of storage. Moreover, malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase, (SOD) and catalase (CAT) levels were measured at 72 h of storage. The enrichment of TEY with SEE at 40 and 80 µg/mL, improved sperm total motility at 48 and 72 h of storage (P

Keywords:

Liquid storage, Ram semen, Spirulina, Semen quality,

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