Zekiye BAŞÇİFTÇİ, Murat OLGUN, Serap SUNAR, Nalan YILDIRIM, Nazife AYTER, Güleray AĞAR

Determination of genetic divergence in some bread wheat varieties by IRAP and ISSR analyses

Bu çalışmada ekmeklik buğday genotiplerindeki genetik uzaklık ölçümü yapılarak genotip farklılıklarının tespiti ve kromozom haritalamasının ıslah programları, elit tohum üretimleri gibi farklı amaçlar için kullanılabilirliğinin gösterilmesi amaçlanmıştır.12 ekmeklik buğday genotipli ekmeklik buğday genotiplerinin (Bozkır, Harmankaya 99, Altay 2000, Yıldırım, Bezostaja 1, Ahmetağa, Müfitbey, Aldane, Es 26, Alperbey, Atay 85 ad Eraybey) genetik çeşitliliği ISSR ve IRAP teknikleri ile incelenmiştir. Hızlı moleküler teknikler olarak, ISSR ve IRAP yöntemleri, hem genetik keşif ve değerlendirme hem de seçkin yetiştirme ve üretim materyallerinin korunması için ıslah programlarına etkin bir şekilde dahil edilebilir. ISSR ve IRAP belirteçleri kullanılarak üretilen dendrogramda 12 genotip iki ana küme halinde sınıflandırıldı. İlk küme Bozkır, Harmankaya 99, Altay 2000, Yıldırım, Bezostaja 1, Ahmetağa ve Müfitbey'i içerirken, ikinci Küme Aldane, Es 26, Alperbey, Atay 85 ve Eraybey'i içermektedir. Ayrıca bu çalışmada elde edilen sonuçlar ISSR ve IRAP yöntemlerinin ekmeklik buğday genotiplerinin tanımlanmasında etkili olduğunu göstermiştir.

Anahtar Kelimeler:

Ekmeklik buğday genotipleri, genetik farklılık, IRAP, ISSR.

Determination of genetic divergence in some bread wheat varieties by IRAP and ISSR analyses

In this study, it was aimed to detect the differences of genotypes through the measurement of genetic distance in bread wheat genotypes and to show the usability of chromosome mapping for different aims such as breeding programs, elite seed productions. The genetic diversity of 12 bread wheat genotypes bread wheat genotypes (Bozkır, Harmankaya 99, Altay 2000, Yıldırım, Bezostaja 1, Ahmetağa, Müfitbey, Aldane, Es 26, Alperbey, Atay 85 ad Eraybey) was examined through ISSR and IRAP techniques. As fast molecular techniques, ISSR and IRAP methods can be effectively included in breeding programs both for genetic exploration and evaluation and for the protection of elite breeding and production materials. 12 genotypes were classified into two main clusters in the dendrogram produced by using the ISSR and IRAP markers. While Cluster I included Bozkır, Harmankaya 99, Altay 2000, Yıldırım, Bezostaja 1, Ahmetağa and Müfitbey, cluster II included Aldane, Es 26, Alperbey, Atay 85 and Eraybey. Furthermore, the results obtained in this study indicated that ISSR and IRAP methods were effective for the definition of bread wheat genotypes.

Keywords:

Bread wheat genotypes, genetic difference, IRAP, ISSR,

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