Stachys macrantha (K.Koch) Stearn’ın Biyolojik Aktivitesinin Belirlenmesi

Stachys, Lamiaceae familyasının en geniş yayılış gösteren cinslerinden birisidir. Bu cinse ait türler biyolojik aktiviteleri ve etnomedikal kullanımları ile bilinmektedir. Bu çalışmada, Stachys macrantha’nın toprak üstü kısımlarının farklı çözücülerle (etanol, metanol ve etil asetat) elde edilen ekstraktlarının toplam fenolik ve flavonoid içeriği ile antioksidan (ABTS, DPPH) ve enzim inhibisyon (α-amilaz, α-glukozidaz, bütirilkolinesteraz ve asetilkolinesteraz) aktiviteleri spektrofotometrik yöntemlerle araştırılmıştır. Ayrıca, fenolik bileşen analizi HPLC ile analiz edilmiştir. Bulgulara göre, metanol ekstraktının diğer ekstraktlara kıyasla biyolojik aktivitelerinin dikkat çekici olduğu bulunmuştur. Ekstraktın, toplam fenolik (45.57 mg GAE/g ekstrakt) ve flavonoid (54.48 mg QE/g ekstrakt) içeriği, antioksidan özellikleri [ABTS (49.99 mg TE/g ekstrakt), DPPH (23.62 mg TE/g ekstrakt)] ile enzim inhibisyon aktiviteleri [α-amilaz (159.35 mmol ACE/g ekstrakt), α-glukozidaz (8.78 mmol ACE/g ekstrakt), bütirilkolinesteraz (5.85 mmol ACE/g ekstrakt) ve asetilkolinesteraz (1.25mmol ACE/g ekstrakt)] belirlenmiştir. Metanol ekstraktının majör fenolik bileşenlerinin kaemferol (539.65 µg/g ekstrakt) ve kuersetin (271.65 µg/g ekstrakt) olduğu tespit edilmiştir. Elde edilen sonuçlara göre, bitkinin tespit edilen aktiviteleri sayesinde ilaç, gıda ve kozmetik endüstrilerinde kullanılma potansiyeli olduğu söylenebilir. Bununla birlikte, söz konusu verilerin daha ileri çalışmalarla desteklenmesi ve aktif bileşenlerin saflaştırılarak etkilerinin in vivo ortamlarda belirlenmesi gerekmektedir.

Determining of Biological Activity of Stachys macrantha (K.Koch) Stearn

Stachys is one of the most widely distributed genera of the Lamiaceae family. Species of this genus are known for their biological activities and ethnomedical uses. In this study, the total phenolic and flavonoid content, antioxidant (ABTS, DPPH) and enzyme inhibition activities (α-amylase, α-glucosidase, butyrylcholinesterase and acetylcholinesterase) of the extracts of the aerial parts of Stachys macrantha obtained with different solvents (ethanol, methanol and ethyl acetate) were investigated by spectrophotometric methods. In addition, phenolic component analysis was analyzed by HPLC. According to the findings, the biological activities of the methanol extract were found to be remarkable compared to other extracts. Total phenolic (45.57 mg GAE/g extract) and flavonoid (54.48 mg QE/g extract) content, antioxidant properties [ABTS (49.99 mg TE/g extract), DPPH (23.62 mg TE/g extract)] and enzyme inhibition activities [α-amylase (159.35 mmol ACE/g extract), α-glucosidase (8.78 mmol ACE/g extract), butyrylcholinesterase (5.85 mmol ACE/g extract), and acetylcholinesterase (1.25 mmol ACE/g extract)] of the extract were determined. The major phenolic components of the methanol extract were determined to be kaempferol (539.65 µg/g extract) and quercetin (271.65 µg/g extract). According to the results obtained, it can be said that the plant has the potential to be used in the pharmaceutical, food and cosmetics industries thanks to the detected activities. However, these data need to be supported by further studies and the effects of the active ingredients should be purified and their effects should be determined in vivo.

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