Mevlüt BAYRAKCI, Bahar YILMAZ, Pelin YEŞİLDAŞ

Antibakteriyel ve Antikanser Özellikleri Gösteren Piridin ve Kinolin Temelli Schiff Bazı Türevlerinin Sensör Özellikleri

Bu çalışmada, literatürdeki güncel sentez yöntemleri izlenerek, piridin ve kinolin grupları içeren Schiff bazı tipinde üç farklı molekül (P-1, P-2 ve P-3) başarıyla sentezlendi. Sentezlenen bileşiklerin yapısal karakterizasyonu spektrofotometrik yöntemler kullanılarak aydınlatıldı. Spektrofotometrik çalışmalarda, P-1 molekülü civaya karşı seçimli bir bağlanma gösterirken, P-2 molekülünün alüminyuma karşı seçimli bir bağlanma sergilediği görüldü. Ayrıca, sentezlenen tüm bileşiklerin antikanser aktivitesi, in vitro koşullarda insan meme kanseri hücresi (MCF7) ve normal insan embriyonik böbrek hücresi (HEK 293) üzerinde Alamar mavisi testi kullanılarak değerlendirildi. Antikanser aktivite sonuçlarına göre, moleküllerin MCF-7 kanser hücre hattı üzerinde daha fazla ölüme neden olduğu görüldü. Dahası, P-1, P-2 ve P-3 moleküllerinin antibakteriyel çalışmaları disk difüzyon yöntemi kullanılarak gram-pozitif (B. subtilis) ve gram-negatif (E. coli) bakteri hatları üzerinde başarılı bir şekilde yapıldı. Bu sonuçlar, P-1 ve P-2'nin canlı hücrelerde sağlıklı hücrelere zarar vermeden mükemmel floresans probları olarak kullanılabileceğini gösterdi. Ayrıca, bu bileşikler Schiff baz moleküllerinin antikanser ve antibakteriyel uygulamaları ile ilgili yeni çalışmalar için ilham kaynağı olabilir.

Sensor Properties of Pyridine and Quinoline Based Schiff Bases Derivatives Showing Antibacterial and Anticancer Properties

In this study, three different Schiff base type molecules (P-1, P-2, and P-3) containing pyridine and quinoline groups were successfully synthesized by following current synthesis methods in the literature. The structural characterization of the synthesized compounds was determined using spectrophotometric methods. In spectrophotometric studies, it was seen that the P-1 molecule had a selective binding against mercury, while the P-2 molecule had a selective binding against aluminum. In addition, the anticancer activity of all the synthesized compounds was been evaluated in vitro model using Alamar blue assay on the human breast adenocarcinoma (MCF-7) and normal human embryonic kidney cell (HEK 293). According to the results of anticancer activity, the molecules were shown to cause more deaths on the MCF-7 cancer cell line. Moreover, antibacterial studies of P1, P-2, and P-3 molecules were successfully performed on gram-negative (E. coli) and gram-positive (B. subtilis) bacteria lines using disc diffusion method. These results showed that P-1 and P-2 can be used as excellent fluorescent probes in living cells without damage to healthy cells. And also, these molecules can be an inspiration for new studies related to the anticancer and antibacterial application of Schiff base molecules.

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