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Limonda Antraknoz hastalığı etmeni Colletotrichum gloeosporioides (Penz.) Penz. & Sacc.’in bazı fungisitlere karşı in vitro duyarlılığı

Antraknoz, dünyada ve ülkemizde limon başta olmak üzere turunçgil üretimini ciddi düzeyde tehdit eden hastalıklardan birisidir. Bu çalışmanın amacı, limonda Antraknoz hastalığı etmeni Colletotrichum gloeosporioides’in DMI, SDHI, QoIs, amid, aniline ve dithiocarbamate gibi farklı kimyasal gruplardan farklı etki mekanizmalarına sahip bazı fungisitlere karşı duyarlılığını belirlemektir. Bu amaçla, limon bahçelerinden toplanan hastalıklı bitkilerin doku parçaları, PDA besi yerinde 25 °C’de 5 gün inkübe edilmiştir. Fungal kültürlerin morfolojik ve moleküler teşhisleri sonucunda tüm izolatların, Colletotrichum gloeosporioides olduğu belirlenmiştir. Patojenisite denemelerinde tüm izolatların virülenslik düzeyleri belirlenmiş ve en yüksek virülensliğe LC5 izolatının sahip olduğu tespit edilmiştir. C. gloeosporioides LC5 izolatının 10 farklı fungisite karşı duyarlılıkları, in vitro’da miselyal gelişim ve konidiyal çimlenme denemeleri ile ortaya konmuştur. Sonuç olarak en yüksek etkiyi, fluopyram+tebuconazole, fluazinam, fluxapyroxad+difenoconazole ve dithianon+fosforoz asit fungisitlerinin gösterdiği belirlenmiştir.

Anahtar Kelimeler:

Limon, Antraknoz, Colletotrichum, Fungisit, Duyarlılık

In vitro sensitivity of anthracnose disease agent, Colletotrichum gloeosporioides (Penz.) Penz. & Sacc., to some fungicides on lemon

Anthracnose is one of the diseases that seriously threaten citrus production, especially lemons in the world and in our country. The aim of this study was to determine the sensitivity of an anthracnose disease agent, Colletotrichum gloeosporioides, on lemon, to some fungicides with different mechanisms of action from different chemical groups such as DMI, SDHI, QoI, amides, anilines and dithiocarbamates. For this purpose, tissue pieces of diseased plants collected from lemon orchards were incubated in PDA medium at 25 °C for 5 days. All isolates were identified as Colletotrichum gloeosporioides as a result of morphological and molecular diagnoses of fungal cultures. The virulence levels of all isolates were determined in pathogenicity tests and the highest virulence was found to have LC5 isolate. The susceptibility of C. gloeosporioides LC5 isolate to 10 different fungicides was demonstrated by in vitro mycelial growth and conidial germination trials. As a result, it was determined that fungicides fluopyram+tebuconazole, fluazinam, fluxapyroxad+ difenoconazole and dithianon+phosphorous acid showed the highest effect.

Keywords:

Lemon, Anthracnose, Colletotrichum, Fungicides, Sensitivity,

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Alexander S.A., Waldenmaier C.M., 2002. Management of anthracnose in bell pepper. Fungicide and Nematicide Tests. Vol. 58. New Fungicide and Nematicide Data Committee of the American Phytopathological Society. p. 49.
Bosland P.W., Votana E.J., 2003. Peppers: Vegetable and Spice Capsicums. CAB International, England, 233 p.
FAO 2016. Statistics of Food and Agriculture Organization of the United Nations, 2016 http://www.fao.org/faostat/ en/#data (01.10.2018)
Gao Y.Y., He L.F., Li B.X., Mu W., Lin J., Liu F., 2017. Sensitivity of Colletotrichum acutatum to six fungicides and reduction in incidence and severity of chili anthracnose using pyraclostrobin. Australasian Plant Patholology, 46, 521–528.
Gao Y., He L., Mu W., Li B., Lin J., Liu F., 2018. Assessment of the baseline sensitivity and resistance risk of Colletotrichum acutatum to fludioxonil. European Journal of Plant Pathology, 150, 639–651.
Guarnaccia V., Groenewald J.Z., Polizzi G., Crous P.W., 2017. High species diversity in Colletotrichum associated with citrus diseases in Europe. Persoonia, 39, 32–50.
Hadden J.F., Black L.L., 1989. Anthracnose of pepper caused by Colletotrichum spp. Proceeding of the International Symposium on Integrated Management Practices: Tomato and pepper production in the tropics. Asian Vegetable Research and Development Centre. Taiwan, p.189-199.
Harp T., Kuhn P., Roberts P.D., Pernezny K.L., 2014. Management and cross-infectivity potential of Colletotrichum acutatum causing anthracnose on bell pepper in Florida. Phytoparasitica, 42, 31–39.
Ishii H., Zhen F., Hu M., Lib X., Schnabel G., 2016. Efficacy of SDHI fungicides, including benzovindiflupyr, against Colletotrichum species. Pest Management Science, 72, 1844–1853.
Kloutvorova J., Egert P., 2004. New possibilities for protecting stone fruits with the fungicide Horizon 250 EW. Online Courier.
Lewis I.M.L., Miller S.A., 2003. Evaluation of fungicides and a biocontrol agents for the control of anthracnose on green pepper fruit, 2003. Nematicide Test Report. Vol. 58. New Fungicide and Nematicide Data Committee of the American Phytopathological Society, p. 62.
Piccirillo G., Carrieria R., Polizzib G., Azzaroc A., Lahoza E., Fernández-Ortuñod D., Vitaleb A., 2018. In vitro and in vivo activity of QoI fungicides against Colletotrichum gloeosporioides causing fruit anthracnose in Citrus sinensis. Scientia Horticulturae, 236, 90–95.
Ramos A.P., Talhinhas P., Sreenivasaprasad S., Oliveira H., 2016. Characterization of Colletotrichum gloeosporioides, as the main causal agent of citrus anthracnose, and C. karstii as species preferentially associated with lemon twig dieback in Portugal. Phytoparasitica, 44, 549–561.
Rhaiem A., Taylor P.W.J., 2016. Colletotrichum gloeosporioides associated with anthracnose symptoms on citrus, a new report for Tunisia. European Journal of Plant Pathology, 146, 219–224.
Uysal A., Kurt Ş., Akgül D.S., 2016. Akdeniz Bölgesi limon bahçelerinde antraknoz hastalığına neden olan Colletotrichum gloeosporioides’in patojenik ve moleküler karakterizasyonu. Uluslararası Katılımlı Türkiye VI. Bitki Koruma Kongresi, 5-8 Eylül, Konya, 597 s.
Vieira W.A.S., Lima W.G., Nascimento E.S., Michereff S.J., Reis A., Doyle V.P., Camara M.P.S., 2017. Thiophanate methyl resistance and fitness components of Colletotrichum musae isolates from Banana in Brazil. Plant Disease, 101,1659-1665.