Genetic diversity among 15 mungbean genotypes of Pakistan was assessed through Random Amplified Polymorphic DNA (RAPD) analysis, with 30 random decamer primers using polymerase chain reaction (PCR). A total of 370 bands were observed, with 12.3 bands per primer, of which 91.6% were polymorphic. OPG-08 produced maximum number of fragments while minimum numbers of fragments were produced with primer OPH-05. Cluster analysis by the Unweighted Paired Group Method of Arithmetic means (UPGMA) showed that these 15 genotypes could be classified in five groups with a similarity ranging from 0.48-0.86. Maximum similarity was observed between NM-51, NM-54 and NM-98 (0.86). Interestingly, these mungbean genotypes have been developed at one breeding center, while ML-5 was found the least similar line due to its exotic nature. The analysis revealed that the inter-varietal genetic relationship of several genotypes is related to their center of origin. Most of the mungbean genotypes have a narrow genetic base. These results correspond well with previous reported results on mungbean from other countries. The RAPD analysis indicated that it may be a more efficient marker than morphological marker, isozyme and RFLP technology. Based on present results, these genotypes could be successfully utilized in selecting divergent parents for breeding and mapping purposes in future.
Afzal, M.A., Haque, M.M., Shanmugasundaram, S. 2004. Random amplified polymorphic DNA (RAPD) analysis of selected mungbean (Vigna radiata L. Wilczek) cultivars. Asian J. Pl. Sci. 3: 20–24.
Arunachalam, V. 1981. Genetic distance in plant breeding. Indian J. Genet. 14: 226-236.
Bisht, I.S., Mahajan R.K., Kawalkar, T.G. 1998. Diversity in mungbean (Vigna radiata L. Wilczek) germplasm collection and its potential use in crop improvement. Ann. Appl. Biol. 132: 301– 312.
Galvan, M.Z., Bornet, B., Balatti, P.A., Branchard, M. 2003. Inter simple sequence repeat (ISSR) markers as a tool for the assessment of both genetic diversity and gene pool origin in common bean (Phaseolus vulgaris L.). Euphytica 132: 297–301.
Ghosh, S., Karanjawala, Z.E., Hauser, E.R. 1997. Methods for precise sizing, automated inning of alleles and reduction or error rates in large scale genotyping using fluorescently labeled dinucleotide markers. Genome Res. 7: 165-178.
Jaccard, P. 1908. Nauvelles recherches sur la distribution florale. Bull Soc Vaudoise Sci Nat. 44: 223-270.
Lakhanpaul, S., Chadha, S., Bhat, K.V. 2000. Random amplified polymorphic DNA analysis in Indian mungbean (Vigna radiata L. Wilczek) cultivars. Genetica. 109: 227–234.
MINFAL, 2007. Agricultural Statistics of Pakistan.
Multani, D.S., Lyon, B.R. 1995. Genetic fingerprinting of Australian cotton cultivars with RAPD markers. Genome. 38: 1005-1008.
Muthusamy, S., Kanagarajan, S., Shanmugasundaram, P. 2008. Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces. Electronic J. Biotech. 3 (11): 1-10.
Newbury, H.J., Ford-Lloyd, B.V. 1993. The use of RAPD for assessing variation in plants. Plant Growth Regulation. 12: 43-51.
Roopa, L.G., Jyoti, S., Shirish, A.R. 2008. Molecular assessment of genetic diversity in mungbean germplasm. J. Genet. (87) 1: 65-74.
Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W. 1984. Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Nat. Acad. Sci., USA. 81: 8014-8018.
Saini, A., Reddy, K.S., Jawali, N. 2004. Evaluation of long primers for AP-PCR analysis of mungbean [Vigna radiata L. Wilczek]: Genetic relationships and finger printing of some genotypes. Indian J. Biotech. 3: 511–518.
Santalla, M., Power, J.B., Davey, M.R. 1998. Genetic diversity in mungbean germplasm revealed by RAPD markers. Plant Breed. 117: 473–478.
Smartt, J. 1985. Evolution of grain legumes III. Pulses in the genus Vigna. Exp. Agric. 21: 87-100.
Vavilov, N.I. 1951. The origin, variation, immunity and breeding of cultivated plants. (Translated by K.S Chester). Chronica Botanica 13: 1-364.
Williams, J.G.K., Kubelik, A.R., Levak, K.J., Rafalski, J.A., Tingey, S.V. 1990. DNA polymorphism amplification by arbitrary primers are useful as genetic markers. Nucleic Acid Res. 18: 6531-6535.