Türkiyede Yetişen Phedimus stoloniferus Türünün Uçucu Kimyasal Bileşimi ve Biyoaktivite Değerlendirilmesi

Bu araştırmada, Türkiye'de yetişen Phedimus stoloniferus'un (Crassulaceae) esansiyel yağında (EO), SPME ve n-hekzan özütünün SPME'sindeki uçucu organik bileşiklerin (VOC) GC-FID/MS ile analiz edilmiştir. P. stoloniferus'un EO'daki uçucu bileşiklerin ana kimyasal sınıfını karboksilik asitler oluşturur (%50.2). P. stoloniferus'un SPME ve heksan özütlerinin ve SPME’leri, ana bileşenlerinin aromatik bileşikler (sırasıyla %51.9 ve %65.9) olduğunu gösterdi. P. stoloniferus'tan elde edilen EO, SPME ve heksan özütlerinin SPME'sindeki ana bileşikler sırasıyla n-Heksadekanoik asit (%34.0), 1-metil-4-etilbenzen (%20.2) ve mesitilen (%28.8)’dir. P. stoloniferus'dan elde edilen esansiyel yağlarda ve çözücü (n-heksan, metanol ve su) özütlerinde antioksidan ve tirozinaz inhibisyonları da incelenmiştir. P. stoloniferus'un metanol özütünde antioksidan aktivite en yüksek olarak (TEAC: 1.23± 0.04) tespit edilmişken, en yüksek tirosinaz enzimi inhibisyonu ise sulu özütte (IC50: 0.168 ± 0.018) gözlendi.

Bioactivity Evaluation and Volatile Chemical Composition of Phedimus stoloniferus Species Grown in Turkey

In this research, volatile organic compounds (VOCs) in the essential oil (EO), SPME and SPME of n-hexane extract of Phedimus stoloniferus (Crassulaceae) grown in Turkey were determined by GC-FID/MS. Major chemical class for the volatile compounds in the EO of P. stoloniferus is carboxylic acids (50.2%). SPME and SPME of the hexane extracts of P. stoloniferus revealed aromatic compounds (51.9% and 65.9%, respectively) as the main constituents. n-Hexadecanoic acid (34.0%), 1-methyl-4-ethylbenzene (20.2%), and mesitylene (28.8%) were the major compounds in the EO, SPME and SPME of hexane extract obtained from P. stoloniferus, respectively. Antioxidant and tyrosinase inhibitions of the essential oil and n-hexane, methanol, and water extracts obtained from P. stoloniferus were also studied. While the antioxidant activity was determined to be highest in methanol extract (TEAC: 1.23±0.04), tyrosinase enzyme inhibition was highest in the aqueous extract (IC50: 0.168±0.018) of P. stoloniferus.

Keywords:

Crassulaceae, Phedimus stoloniferus, SPME, GC-FID/MS, Antioxidant Tyrosinase Inhibition,

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