The Effect of Vitamin D3 on Skeletal Muscle in Rat after Ischemia Reperfusion Injury: Preliminary Report

Objective: Vitamin D is a vitamin that has gained popularity in recent years and has an anti-inflammatory and immunmodular effect. In the literature review we conducted, there was no study investigating the effect of vitamin D to prevent ischemia reperfusion (I/R) injury in skeletal muscle. The aim of this study was to investigate the effect of vitamin D on skeletal muscle in I/R injury. Methods: Six rats were used in each group including ischemia group (group I) and experimental group (group D given vitamin D). In the histopathological examination, inflammation and apoptosis levels were studied in gastrocnemius muscle. In biochemical analysis, total oxidative stress and total antioxidant and catalase levels were evaluated in anterior tibialis muscle. Results: The number of apoptotic cells in group D were found significantly lower than group I. In the terms of total antioxidants and catalase levels, there were significant difference between group D and group I. Conclusion: Our results support the hypothesis that vitamin D is an agent that can be used to prevent I/R injury.

Sıçan İskelet Kasında İskemi Reperfüzyon Hasarı Sonrası Vitamin D3’ün Etkisi: Ön Çalışma

Amaç: Vitamin D, son yıllarda giderek popülarite kazanan ve antienflamatuvar ve immünomodülatör etkiye sahip bir vitamindir. Yaptığımız literatür taramasında iskelet kasında iskemi reperfüzyon (İ/R) hasarını önlemeye yönelik vitamin D’nin etkisini inceleyen bir çalışma bulunamamıştır. Çalışmamızda; iskemi reperfüzyon hasarında vitamin D’nin iskelet kası üzerindeki etkisinin araştırılması amaçlanmıştır. Yöntemler: İskemi grubu ve deney grubu (D vitamin verilen) olmak üzere her grupta 6 adet, toplam 12 adet sıçan kullanılmıştır. Histopatolojik incelemede gastroknemius kasında enflamasyon, apopitozis düzeyi araştırıldı. Biyokimsayal analizde ise anterior tibialis kasında total oksidatif stres, total antioksidan ve katalaz seviyelerine bakıldı. Bulgular: Apoptotik hücre sayısının, D vitamin uygulanan grupta kontrol grubuna göre anlamlı olarak daha az olduğunu saptandı. Katalaz ve total antioksidan seviyeleri açısından Grup D ve Grup İ arasında anlamlı fark saptanmıştır. Sonuç: Bu çalışmada iskelet kası İ/R hasarına vitamin D’nin olumlu yönde etki gösterdiği saptanmıştır.

Kaynakça

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