Investigation of the Effects of Isotretinoin on Spermatogenesis in Balb/c Mice

Objective: It was aimed to investigate the effect of isotretinoin (ISR), a retinoid derivative of vitamin A, on spermatogenesis via follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone levels. Methods: Twelve male Balb/c mice were divided into two groups: Control and ISR groups. FSH, LH and testosterone levels were biochemically determined by ELISA assay. All statistical analyses were made with SPSS 20.0 (IBM, New York, USA) and graphics were prepared with Graph Pad Prism Version 6.01 (USA). The parametric Student’s t-test was applied for comparison among groups. A p value ≤0.05 was accepted as statistically significant. Results: Mean FSH, LH and testosterone levels were increased in the ISR group when compared to those of control group. The increases in all hormone levels were found statistically significant between the two groups (p

Balb/c Cinsi Farelerde İzotretinoinin Spermatogenez Üzerine Etkilerinin Araştırılması

Amaç: Vitamin A’nın bir retinoid türevi olan izotretinoinin (ISR) spermatogenez üzerindeki etkisinin follikül stimüle edici hormon (FSH), luteinize edici hormon (LH) ve testosteron seviyeleri üzerinden araştırılması amaçlandı. Yöntemler: Bu çalışma 12 adet Balb/c cinsi 3 aylık erkek fare ile gerçekleştirildi. Deney grupları kontrol (n=6) ve ISR (n=6) olmak üzere ikiye ayrıldı. Serum örneklerinde FSH, LH ve testosteron seviyeleri ELISA yöntemiyle belirlendi. Tüm analizler ve grafikler için SPSS 20,00 (IBM, New York, USA) ve Graph Pad Prism Version 6,01 (USA) programları kullanıldı. İstatistiki karşılaştırılmalarda parametrik student t-testinden yararlanıldı. Anlamlılık seviyesi p≤0,05 olarak kabul edildi. Bulgular: ISR grubunda ortalama FSH, LH ve testosteron seviyelerinde kontrol grubuna göre artış belirlendi. Bu artış her üç hormon için de iki grup arasında istatistiksel açıdan anlamlı bulundu (p

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