Erzurum Bölgesinde Yetişen Betula pendula Yapraklarının Antioksidan Kapasitelerinin Belirlenmesi ve Kemometrik Karakterizasyonu

Bu çalışmada, Erzurum bölgesinin karakteristik bitki örtüsü içerisinde yer alan Huş Ağacına ait farklı yerleşimlerden alınan yaprak örnekleri farklı in vitro antioksidan kapasite yöntemleri ile incelenmiş ve kemometrik karakterizasyonu yapılmıştır. Uzundere, Horasan ve Şenkaya bölgelerinde mayıs-temmuz aralığında toplanan örnekler kurutulmuş ve etanol ekstreleri çıkarılmıştır. Bakır II iyonu İndirgeyici Antioksidan Kapasite (CUPRAC), Demir III iyonu İndirgeyici Antioksidan Kapasite (FRAP) ve Difenil pikril Hidrazin (DPPH) yöntemleri için gerçekleştirilen analizlere bütün örnekler pozitif yanıt vermişlerdir. En yüksek antioksidan kapasite değerlerine Şenkaya bölgesinden elde edilen örneklerle ulaşılmıştır. Elde edilen değerler bilgisayar algoritmaları yardımıyla kemometrik olarak incelendiğinde; sonuçların anlamlı farklılık gösterdiği ve her bölgede rakım değişimiyle pozitif korelasyon gösteren farklılıklar tespit edilmiştir. Yapılan çalışma vasıtasıyla antioksidan kapasite değerleri ile coğrafi karakterizasyonun gerçekleştirilebileceği ve rakımsal farklılıkların Huş ağacı yapraklarının fenolik ve flavanoid içeriğine anlamlı etki yaptığı sonucuna varılmıştır. Huş ağacı bitkisinin antioksidan kapasitesi sebebiyle özellikle kanser ve kozmetik gibi önemli alanlarda kullanılabileceği düşünülmektedir.

Anahtar Kelimeler:

Antioksidan Kapasite, Huş Ağacı Yaprağı, CUPRAC, FRAP, DPPH

Determination of Antioxidant Activity and Chemometric Characterization of Betula pendula Leaves in Erzurum Region

In this study, leaf samples of Birch Tree collected from different settlements in the characteristic vegetation of Erzurum region were investigated with different in vitro antioxidant activity investigated and chemometrics characterization was carried out. The samples were harvested in the May-July period from Uzundere, Horasan and Şenkaya regions, dried and their ethanol extracts were obtained. CUPRAC, FRAP and DPPH responses were positive for all samples. The highest antioxidant capacity values were obtained from samples of Şenkaya region. When the obtained values are examined chemometrically with the help of computer algorithms; significant differences and a positive correlation with the altitude change in each region were obtained. It was concluded that geographic characterization can be realized by antioxidant activity values and the altitude differences have a significant effect on phenolic and flavanoid contents of birch leaves. Owing to the antioxidant activity of the birch plant it is thought that it can be used especially in important areas such as cancer and cosmetics.

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