Arzu YAY, Hasan AKŞİT, Dilek AKŞİT, Kamil SEYREK, Adil KILIÇ, Esin S SARI, Sıtkı S ERMİŞ, Alper YAZICI, Onur YILDIZ

Selenium Protects Retinal Cells from Cisplatin-Induced Alterations in Carbohydrate Residues

Background: Investigate alterations in the expression and localization of carbohydrate units in rat retinal cells exposed to cisplatin toxicity. Aims: The aim of the study was to evaluate putative protec-tive effects of selenium on retinal cells subjected to cisplatin. Study Design: Animal experiment.Methods:EighteenhealthyWistarratsweredividedinto three equal groups: 1. Control, 2. Cisplatin and 3. Cisplatin+selenium groups. After anesthesia, the right eye of each rat was enucleated. Results: Histochemically, retinal cells of control groups reacted with ?-2,3-bound sialic acid-specific Maackia amu-rensis lectin (MAA) strongly, while cisplatin reduced the staining intensity for MAA. However, selenium administra-tion alleviated the reducing effect of cisplatin on the bind-ing sites for MAA in retinal cells. The staining intensity for N-acetylgalactosamine (GalNAc residues) specific Griffo-nia simplicifolia-1 (GSL-1) was relatively slight in control animals and cisplatin reduced this slight staining for GSL-1 further. Selenium administration mitigated the reducing ef-fect of cisplatin on the binding sites for GSL-1. A diffuse staining for N-acetylglucosamine (GlcNAc) specific wheat germ agglutinin (WGA) was observed throughout the retina of the control animals. In particular, cells localized in the in-ner plexiform and photoreceptor layers are reacted strongly with WGA. Compared to the control animals, binding sites for WGA in the retina of rats given cisplatin were remark-ably decreased. However, the retinal cells of rats given sele-nium reacted strongly with WGA. Conclusion: Cisplatin reduces ?-2,3-bound sialic acid, GlcNAc and GalNAc residues in certain retinal cells. How-ever, selenium alleviates the reducing effect of cisplatin on carbohydrate residues in retinal cells

PDF

___

Carlson BA, Yoo MH, Shrimali RK, Irons R, Gladyshev VN, Hatfield DL, et al. Role of selenium-containing proteins in T- cell and macrophage function. Proc Nutr Soc 2010;69:300-10. [Crossref]
Zhang ZW, Wang QH, Zhang JL, Li S, Wang XL, Xu SW. Effects of oxidative stress on immunosuppression induced by selenium deficiency in chickens. Biol Trace Elem Res 2012;149:352-61. [Crossref]
Rayman MP. The importance of selenium to human health. Lan- cet 2000;356:233-41. [Crossref]
Puertas MC, Martinez-Martos JM, Cobo MP, Carrera MP, Ma- yas MD, Ramirez-Exposito MJ. Plasma oxidative stress param- eters in men and women with early stage Alzheimer type de- mentia. Exp Gerontol 2012;47:625-30. [Crossref]
Thuluvath PJ, Triger DR. Selenium in chronic liver disease. J Hepatol 1992;14:176-82. [Crossref]
Franz H. Mistletoe lectins and their A and B chains. Oncology 1986;43:23-34. [Crossref]
Gabius HJ. Tumorlectinologie: Ein gebiet im schnittpunkt von zuckerchemie, biochemie, zellbiologie und onkologie. Angew Chem Int Edit 1988;100:1321-30. [Crossref]
Argueso P. Glycobiology of the ocular surface: mucins and lec- tins. Jpn J Ophthalmol 2013;57:150-5. [Crossref]
Bourrillon R, Aubery M. Cell surface glycoproteins in em- bryonic development. Int Rev Cytol 1989;116:257-338. [Crossref]
Summerfield JA. The role of human lectins in host defence. The Linacre Lecture 1991. J Roy Coll Phys Lond 1992;26:92-6.
Kostrominova TY. Application of WGA lectin staining for visu- alization of the connective tissue in skeletal muscle, bone, and ligament/tendon studies. Microsc Res Techniq 2011;74:18-22. [Crossref]
Shin T. The pleiotropic effects of galectin-3 in neuroinflamma- tion: a review. Acta Histochem 2013;115:407-11. [Crossref]
Van der Ende J, Van Baardewijk LJ, Sier CF, Schipper IB. Bone healing and mannose-binding lectin. Int J Surg 2013;11:296- 300. [Crossref]
Amin A, Buratovich MA. New platinum and ruthenium com- plexes-the latest class of potential chemotherapeutic drugs-a review of recent developments in the field. Mini-Rev Med Chem 2009;9:1489-503. [Crossref]
Gregg RW, Molepo JM, Monpetit VJ, Mikael NZ, Redmond D, Gadia M, et al. Cisplatin neurotoxicity: the relationship be- tween dosage, time, and platinum concentration in neurologic tissues, and morphologic evidence of toxicity. J Clin Oncol 1992;10:795-803.
Yazici ZM, Meric A, Midi A, Arinc YV, Kahya V, Hafiz G. Re- duction of cisplatin ototoxicity in rats by oral administration of pomegranate extract. Eur Arch Oto-Rhino-L 2012;269:45-52. [Crossref]
Ozbal S, Erbil G, Kocdor H, Tugyan K, Pekcetin C, Ozogul C. The effects of selenium against cerebral ischemia-reperfusion injury in rats. Neurosci Lett 2008;438:265-9. [Crossref]
Ognjanovi BI, Djordjevic NZ, Matic MM, Obradovic JM, Mladenovic JM, Stajn AS, et al. Lipid peroxidative damage on Cisplatin exposure and alterations in antioxidant defense system in rat kidneys: a possible protective effect of selenium. Int J Mol Sci 2012;13:1790-803. [Crossref]
Rezvanfar MA, Shahverdi AR, Ahmadi A, Baeeri M, Mohammad- irad A, Abdollahi M. Protection of cisplatin-induced spermatotox- icity, DNA damage and chromatin abnormality by selenium nano- particles. Toxicol Appl Pharm 2013;266:356-65. [Crossref]
Wang PH. Altered sialylation and its roles in gynecologic can- cers. J Cancer Mol 2006;2:107-16.
Roos MD, Hanover JA. Structure of O-linked GlcNAc trans- ferase: mediator of glycan-dependent signaling. Biochem Bioph Res Co 2000;271:275-80. [Crossref]
Manning G, Whyte DB, Martinez R, Hunter T, Sudarsanam S. The protein kinase complement of the human genome. Science 2002;298:1912-34. [Crossref]
Copeland RJ, Bullen JW, Hart GW. Cross-talk between GlcNAcylation and phosphorylation: roles in insulin resis- tance and glucose toxicity. AJP Endocrinology and Metabolism 2008;295:E17-28. [Crossref]
Yazici A, Sari ES, Yay A, Akşit H, Kilic A, Akşit D, et al. The Protective Effect of Selenium in Cisplatin Related Retinotoxic- ity. Cutan Ocul Toxicol 2014;33:327-32. [Crossref]
Kamigaito T, Okaneya T, Kawakubo M, Shimojo H, Nishizawa O, Nakayama J. Overexpression of O-GlcNAc by prostate can- cer cells is significantly associated with poor prognosis of pa- tients. Prostate Cancer P D 2014;1:18-22. [Crossref]
Sadeghian S, Kojouri GA, Mohebbi A. Nanoparticles of Se- lenium as species with stronger physiological effects in sheep in comparison with sodium selenite. Biol Trace Elem Res 2012;146:302-8. [Crossref]
Bellinger FP, Raman AV, Reeves MA, Berry MJ. Regulation and function of selenoproteins in human disease. Biochem J 2009;422:11-22. [Crossref]
Flohe L. Selenium, selenoproteins and vision. In: Nutrition and the eye ed. A, A. Karger; 2005.
Sheck L, Davies J, Wilson G. Selenium and ocular health in New Zealand. N Z Med J 2010;123:85-94.
Schultz MJ, Swindall AF, Wright JW, Sztul ES, Landen CN, Bellis SL. ST6Gal-I sialyltransferase confers cisplatin resis- tance in ovarian tumor cells. J Ovarian Res 2013;6:2-9.