Comparison of culture, real-time dna amplification assay and ehrlich-ziehl-neelsen for detection of mycobacterium tuberculosis
Comparison of culture, real-time dna amplification assay and ehrlich-ziehl-neelsen for detection of mycobacterium tuberculosis
Objective: Mycobacterium tuberculosis is still a substantial health problem universally. Although culture is the gold standard method, reliable, rapid and new methods are required for effective struggle with disease. We retrospectively compared the results of Ehrlich-Ziehl-Neelsen (EZN) stain and real-time DNA amplification assay (BD ProbeTec ET system) with culture. Study Design: Retrospective study. Material and Methods: A total of 703 samples, 182 pulmonary and 521 extra pulmonary, collected from 630 patients between May 2008 and February 2011 were evaluated. Culture was considered the gold standard. Results: For pulmonary specimens, sensitivity, specificity, positive predictive and negative predictive values of BD ProbeTec ET and EZN were calculated to be 100%, 98.8%, 87.5%, 100% and 71.4%, 98.8%, 83.3%, 97.6%, respectively. For extra pulmonary specimens, sensitivity, specificity, positive predictive and negative predictive values of BD ProbeTec ET and EZN were calculated to be 80%, 98.7%, 76.9%, 98.9% and 24%, 98.3%, 42.8%, 96.2%, respectively. Conclusion: According to these results, we suggest that the BD ProbeTec ET system is more reliable than EZN. In addition, the BD ProbeTec ET system produces faster results. Based upon these results, we consider that the BD ProbeTec ET system may be employed in the diagnosis of M. tuberculosis.
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- 1. Forbes BA, Sahm DF, Weissfeld AS. Bailey & Scotts Diagnostic Microbiology. 12. Edition. Missouri:Mosby Elsevier;2007. p.478.
- 2. Babacan F, Hasdemir U, Mycobacterium tuberculosis Kom- pleks. In:Topçu AW, Söyletir G, Doğanay M, editors. Enfeksi- yon Hastalıkları ve Mikrobiyolojisi. 3. Baskı. İstanbul:Nobel Tıp Kitabevleri;2008. p.2283.
- 3. Winn W, Allen S, Janda W, Koneman E, Procop G, Schrecken- berger P. Konemans Color Atlas and Textbook of Diagnostic Microbiology. 6. Edition. Philadelphia:Lippincott Williams & Wilkins;2006. p.1065.
- 4. Kocagöz T, Tüberküloz Tanısında Kullanılan Moleküler Yöntem- ler. In:Ağaçfidan A, Badur S, Türkoğlu S, editors. İnfeksiyon Hastalıklarının Laboratuar Tanısında Moleküler Yöntemler. İstanbul:M.G.G. AS Matbaacılık;2002. p.189.
- 5. Vincent V, Gutierrez NC, Mycobacterium:Laboratory Character- istics of Slowly Growing Mycobacteria. In:Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA, editors. Manual of Clinical Microbiology. 9. Edition. Washington:ASM Press;2007. p.573.
- 6. Saniç A, Çoban AY. Mikobakteriler ve Laboratuar Tanı. Samsun, 1999.
- 7. Wang JY, Lee LN, Hsu HL, Hsueh PR, Luh KT. Performance Assess- ment of the DR. MTBC Screen Assay and the BD ProbeTec ET Sys- tem for Direct Detection of Mycobacterium tuberculosis in Respira- tory Specimens. J Clin Microbiol 2006;44:716-9. [CrossRef]
- 8. Wang JY, Lee LN, Chou CS, Huang CY, Wang SK, Lai HC, et al. Performance Assessment of a Nested-PCR Assay (the RAPID BAP-MTB) and the BD ProbeTec ET System for Detection of My- cobacterium tuberculosis in Clinical Specimens. J Clin Microbiol 2004;42:4599-603. [CrossRef]
- 9. Barrett A, Magee JG, Freeman R. An evaluation of the BD Pro- beTec ET system for the direct detection of Mycobacterium tuber- culosis in respiratory samples. J Med Microbiol 2002;51:895-8.
- 10. Wang SX, Sng LH, Tay L. Preliminary study on rapid identification of Mycobacterium tuberculosis complex isolates by the BD Pro- beTec ET system. J Med Microbiol 2004;53:57-9. [CrossRef]
- 11. McHugh TD, Pope CF, Ling CL, Patel S, Billington OJ, Gosling RD, et al. Prospective evaluation of BDProbeTec strand displacement ampli- fication (SDA) system for diagnosis of tuberculosis in non-respiratory and respiratory samples. J Med Microbiol 2004;53:=
- 12. Köksalan OK. Günümüzde tüberküloz tanısı/güçlükleri. ANKEM Derg 2010;24(Ek 2):61-3.