Punikalaginin Kolistin Dirençli Acinetobacter baumannii Suşlarına Karşı Tek Başına ve Kolistin ile Kombinasyon Halinde Antimikrobiyal Aktivitesinin Değerlendirilmesi

Bu çalışmanın amacı, punikalaginin tek başına ve kolistin ile kombinasyon halinde antimikrobiyal aktivitesini kolistine dirençli A. baumannii suşları üzerinde değerlendirmektir. Çalışmada test edilmek üzere beş adet kolistine dirençli A. baumannii suşu kullanılmıştır. Suşlara karşı punikalagin ve kolistinin antimikrobiyal aktivitelerini saptamak için sıvı mikrodilüsyon testi yapılarak Minimum İnhibisyon Konsantrasyonları (MİK) belirlenmiştir. Bu iki ajanın kombinasyon aktiviteleri ise Dama Tahtası (Checkerboard) Sinerji Testi ile değerlendirilmiştir. Sıvı mikrodilüsyon testinin sonuçlarına göre tüm suşlara karşı punikalaginin MİK değeri 256 µg/ml, kolistinin MİK değerleri ise iki suşta 32 µg/ml, diğer üç suşta 8 µg/ml olarak tespit edilmiştir. Checkerboard sinerji testi ile kombinasyon etkileşimlerinin belirlenmesinde Fraksiyonel İnhibitör Konsantrasyon İndeksi (FİKİ) hesaplanmıştır. Buna göre kolistin ve punikalagin kombinasyonunun FİKİ değerleri iki suş için 1.12, diğer üç suş için ise 1.03 olarak bulunmuştur. Sonuç olarak, punikalagin kolistin dirençli A. baumannii suşları üzerinde antimikrobiyal aktivite göstermiştir. Kolistin ile kombinasyonunun ise tüm test edilen suşlar üzerinde indiferan etkili olduğu tespit edilmiştir. Hiçbir suş üzerinde sinerjistik etki gözlenmemiştir. Bunun yanısıra herhangi bir antagonistik etki de saptanmamıştır.

Anahtar Kelimeler:

Acinetobacter baumannii, kolistin, punicalagin, indiferan etki

Evaluation of the Antimicrobial Activity of Punicalagin Alone and in Combination with Colistin Against Colistin-Resistant Acinetobacter baumannii Strains

The aim of this study was to evaluate the antimicrobial activity of punicalagin alone and in combination with colistin on colistin-resistant A. baumannii strains. Five colistin-resistant A. baumannii strains were used to be tested in the study. Broth microdilution test was performed to evaluate the antimicrobial activities of punicalagin and colistin against strains and Minimum Inhibition Concentrations (MIC) were determined. Combination activities of these two agents were evaluated with the Checkerboard Synergy Test. According to the results of the broth microdilution test, the MIC value of punicalagin against all strains was 256 µg/ml, the MIC values of colistin were found to be 32 µg/ml on two strains and 8 µg/ml on the other three strains. The Fractional Inhibitory Concentration Index (FICI) was calculated to determine combination interactions with the Checkerboard synergy test. Accordingly, the FICI values of the combination of colistin and punicalagin were found as 1.12 for two strains, 1.03 for the other three strains. In conclusion, punicalagin showed antimicrobial activity on colistin-resistant A. baumannii strains. It was determined that the combination with colistin had an indifferent effect on all tested strains. No synergistic effects were observed on any of the strains. In addition, no antagonistic effects were detected.

Keywords:

Acinetobacter baumannii, colistin, punicalagin, indifferent effect,

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