Ahmet Metin KUMLAY, Barış EREN, Serap DEMİREL, Fatih DEMİREL, Bünyamin YILDIRIM

Bazı Pamuk Çeşitlerinde iPBS Analiziyle Genetik Varyasyonun Ortaya Çıkarılması

Bu çalışmada, 10 adet pamuk (Gossypium hirsutum L.) çeşidinin moleküler karakterizasyonu yapılmıştır. DNA temelli 6 adet iPBS markörleri kullanılmış olup, toplamda 36 adet polimorfik bant görülmüş ve ortalama polimorfizm oranı %77.65 olarak saptanmıştır. Markörlerin ortalama polimorfizm değeri (PIC) de 0.27, ortalama gen çeşitliliği (H) değerleri 0.34, ortalama Dice benzerlik katsayı değeri 0.37, korelasyon katsayı değeri (r) de 0.9026 olarak hesaplanmıştır. Genotipler arasındaki genetik ilişki, NTSYS-pc yazılımı kullanılarak belirlenmiş ve 0.0456 ile 0.8387 arasında değiştiği saptanmıştır. İlk üç eigen değerleri toplamı, toplam varyasyonun %71.93’ünü açıklamıştır. Genotiplerin moleküler varyasyon gösterdiği belirlenmiş olup, genetik çeşitlilik sonuçlarının gelecekteki pamuk ıslah çalışmalarının planlanmasında kullanılabileceği sonucuna varılmıştır.

Anahtar Kelimeler:

Gossypium hirsutum L, Polimorfizm, Islah, iPBS

Reveal of Genetic Variation by iPBS Analysis in Some Cotton Varieties

In current study, molecular characterization of 10 cotton (Gossypium hirsutum L.) varieties is performed. There are 6 DNA-based iPBS markers, a total of 36 polymorphic bands were detected and the mean of polymorphism rate was 77.65%. The mean gene diversity (H) of the markers was 0.34 and the mean polymorphism value (PIC) was 0.27. The Dice similarity coefficient value and correlation coefficient was calculated as 0.337 and 0.9026, respectively. Genetic diversity among cotton genotypes using NTSYS-pc software changed 0.0456 and 0.8387. Sum of the first three Eigen value explained 71.93% of the total variation. The cotton genotypes used in this study showed highly molecular variation. It was concluded that results of genetic diversity could be used in the selection planning in future cotton breeding studies.

Keywords:

Gossypium hirsutum L., polymorphism, breeding, iPBS,

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