KOYUN MASTİTİSLERİ ÜZERİNDE MİKROBİYOLOJİK İNCELEMELER
Bu çalışma, Konya yöresindeki iki devlet kurumuna ait 800 sağmal koyun üzerinde yapıldı. Hayvanlar, Californiü Mastitis Test (CMT) ile ve klinik olarak muayene edilerek, klinik ve subklinik mastilisli memeler tespit edildi. CMT bulgulan ++ ve +++ olan memelerden alınan süt örnekleri aerobik, mikroaerofilik ve anaerobik olarak bakteriyolojik ve mikolojik yönden incelendi. Ayrıca izole ve idenlifiye edilen bakterilerin antibiyotiklere duyarlılıkları saptandı. Antibiyotiklere duyarlılık testlerinde; Ampisilin, Streptomisin, Penisilin, Kanamisin, Eritromisin, Gentamisin, Tetrasiklin ve Kloramfenikol diskleri (Difco) kullanıldı. Bölgede CMT ile taranan 800 koyunun 108'i (% 13.5) mastilisli olarak saptandı. Bu 108 koyunun 102'si (% 12.75) subklinik, 6'sı (% 0.75) klinik mastitisli bulundu. Mastilisli süt örneklerinin 81'inden (% 75.0) patojen mikroorganizmalar üretildi. Süt örneklerinin 8'inde miks infeksiyon tespit edildi. lzole edilen mikroorganizmaların yüzde oranlan sırasıyla; Staph. aweus 57.3, E. coli 9.0, Bacillus ssp 7.9, B. cereus 5.0, C. pyogenes (Actinomyces pyogenes) 5.0, Str. agalactiae 3.4, K. pneumoniae 3.4, Candida albicans 3.4, B. subtilis 2.2, Str. dysgalactiae 1.1 ve Entero - hacter ssp. 1.1 olarak saptandı. Antibiyogram testlerinde Staph. aureus; Tetrasiklin'e % 76.0, Kloramfenikol'e% 58.0 ve Eritromisin'e % 38.0, E. coli; Streptomisin ve Tetrasiklin'e% 100, C. pyogenes; Penisilin'e % 100, Streptomisin ve Kloramfenikol'e % 80.0 oranında duyarlı bulundu.
Microbiologic Studies on Mastitis in Sheep
This investigation was performed on 800 milch sheep belonged two state farm in Konya region. Sheep were examined by elinical procedures and tested with California Mastitis test (CMT). Milk samples taken from sheep with maStitis (CMT scores, ++/+++) w ere cultured in aerobic, microaerophlic and anaerobic conditions for bacteriological and mycological examination bacteria isolated and identified were tested against Ampicillin, Streptomycin, Tetracyclin, Penicillin, Kanamycin, Erytromycin, Gentamyem and Chloramphenicol discs (Difco ). In this area, in the mammary glands of 13.5 % of the -tested sheep, mastitis was detected. Although the rates of elinical mastitis was 0.75 percent, subclinical mastitis in sheep was 12.75 percent Microbiologic isolation were succeded from (75.0 %) 81 samples of 108 mastitic milk. But, there was mix infection in 8 samples. The percent values of isolated microorganisms were found as below : 57.3 % Staph. aureus, 9.0 % for E. coli, 7.9 %for Bacillus ssp. 5.0% for B. cereus, 5.0% for C. pyogenes (Actinomyces pyogenes), 3.4 % for Str. agalactiae, 3.4 % for C. pyogenes (Actinomyces pyogenes), 3.4% for Sir. agalactiae, 3.4% for K. pneumoniae, 3.4 %for Candida albicans, 2.2 % for B. subtilis, 1.1 %for Str. dysgalactiae and 1.1 for Enterobacter ssp. In antibiotic susceptibility tests, Staph. aureus isolates were found to be sensitive to Tetracyclin, Chloramphenicol and Erytromycin, at the rates of 76.0 %, 58.0 % and 38.0 %, respectively. E. coli strains were sensitive to Streptomycin (100 %) and Tetracyclin (100 %), C. pyogenes strains were sensitive at the rates of 100 %, 80.0% and 80.0 %, for Penicillin, Streptomycin and Chloramphenicol, respectively