Erhan ÖNALAN, Nevzat GÖZEL, Emir DÖNDER, Tuncay KULOĞLU, Selçuk DEMİRCAN, Süleyman AYDIN, Mehmet Hanifi YALÇIN

Effects of Vitamin D on adropine and apoptosis in kidney tissue

Aim: This study aims to investigate the effects of vitamin D on adropin and apoptosis in rat kidney tissue in the context of theexperimental diabetes model created using streptozotocin (STZ).Material and Methods: 41 male Wistar-albino breed rats of 8-10 weeks were distributed into 5 groups, which consisted of 3 groupswith 7 animals each and 2 groups with 10 animals each. No treatments were applied to the control group. The Buffer group wasadministered with single-dose 0.1 M sodium buffer intraperitoneally (ip). The Vitamin D group was orally administered 200 IU/dayvitamin D. The Diabetes group was injected ip with single-dose 50 mg/kg STZ by dissolving the material in 0.1 M sodium buffer.Results: The biochemical and histological investigations revealed similar serum TOS and TAS levels, and TUNEL positivity andAdropin immunoreactivity for the Control, Buffer, and Vitamin D groups. While TOS levels and TUNEL positivity were significantlyhigher in the Diabetes group compared to the Control group, TAS levels and Adropin immunoreactivity were significantly lower. TheTOS levels and TUNEL positivity were significantly reduced in the Diabetes+Vitamin D group compared to the diabetic group, and TASlevels, adropin immunoreactivity were significantly higher.Conclusion: In conclusion; it was determined that experimental diabetes increased TOS and apoptotic cells and decreased TASand adropin levels in the kidney tissue in experimental diabetes, and that Vitamin D administered as treatment decreased TOSand apoptotic cells and increased TAS and Adropin levels. It was concluded that in order to uncover the role of diabetes in thepathophysiology of its effect on kidney tissue, future studies that consider various experimental diabetes times were necessary

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1. Aydin S. Three new players in energy regulation: Preptin, adropin and irisin. Peptides. 2014;56:94-110.
2. Fidan F, Alkan B M, Tosun A. Pandemic of the Age: Vitamin D deficiency and deficiency. Turkish J Osteoporosis 2014;20:71-4.
3. Wacker M, Holick M. Vitamin D-effects on skeletal and extraskeletal health and the need for supplementation. Nutrients 2013;5:111-48.
4. Badawi A, Sayegh S, Sadoun E, et al. Relationship between insulin resistance and plasma vitamin D in adults. Diabetes Metab Syndr Obes 2014;7:297-303.
5. Zhou J, Chen H, Wang Z, et al. Effects of vitamin D supplementation on insulin resistance in patients with type 2 diabetes mellitus. Zhonghua yi xue za zhi 2014;94:3407-410.
6. Wiseman H. Vitamin D is a membrane antioxidant: ability to inhibit iron-dependent lipid peroxidation in liposomes compared to cholesterol, ergosterol, and tamoxifen and relevance to anticancer action. FEBS J 1993;326:285-88.
7. Oren M, Rotter V. Introduction: p53 the first twenty years. Cell Mol Life Sci 1999;55:9-11.
8. Erel, OA. Novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004;37,112-19.
9. Erel, OA.New automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38:1103-11.
10. Su J, Sandor K, Sköld K, et al. Identification and quantification of neuropeptides in naïve rat spinal cord using mass spectrometry reveals [des-Ser1]- cerebellin as a novel modulator of nociception. Neurochem 2014;130:199-214.
11. Chacón PJ, del Marco Á, Arévalo Á, et al. RodríguezTébar Cerebellin 4, a synaptic protein, enhances inhibitory activity and resistance of neurons to amyloid-β toxicity. A Neurobiol Aging 2015;36:1057- 71.
12. Cagle MC, Honig MG. Parcellation of cerebellins 1, 2, and 4 among different subpopulations of dorsal horn neurons in rat spinal cord. J Comp Neurol 2014;522: 479-97.
13. Aydin S, Kuloglu T, Aydin S, et al. Expression of adropin in rat brain, cerebellum, kidneys, heart, liver, and pancreas in streptozotocin-induced diabetes.Mol Cell Biochem 2013;380:73-81.
14. Kumar KG, Trevaskis JL, Lam DD, et al. Identification of adropin as a secreted factor linking dietary macronutrient intake with energy homeostasis and lipid metabolism. Cell Metab 2008;8:468-81.
15. Kuloglu T, Aydin S, Eren MN, et al. Irisin: a potentially candidate marker for myocardial infarction. Peptides 2014;55:85-91.
16. Pfaffly JR. Diabetic complications, hyperglicemi and free radicals. Diabetic complications. Neuroscience Research Communications 1997;21:41-8.
17. Van Dam PS, Bravenboer B. Oxidative stres and antioxidant treatment in diabetic neuropathy. Neuroscience Research Communications 1997;21: 41-8.
18. Burçak G, Andican G. Oxidative DNA damage and aging.Cerrahpasa Journal of Medicine 2004;35:159- 69.
19. Oren M, Rotter V. Introduction: p53 the first twenty years. Cell Mol Life Sci 1999;55:9-11.
20. Kumar KG, Trevaskis JL, Lam DD, et al. Identification of adropin as a secreted factor linking dietary macronutrient intake with energy homeostasis and lipid metabolism. Cell Metab 2008;8:468-81.
21. Kumar KG, Zhang J, Gao S, et al. Adropin deficiency is associated with increased adiposity and insulin resistance. Obesity (Silver Spring) 2012;20:1394-402.
22. Lovren F, Pan Y, Quan A. Adropin is a novel regulator of endothelial function. Circulation 2010;122:185-92.
23. Gao S, McMillan RP, Jacas J, et al.Regulation of substrate oxidation preferences in muscle by the peptide hormone adropin. Diabetes 2014;63:3242-52.
24. Gao S, McMillan RP, Zhu Q, et al. Therapeutic effects of adropin on glucose tolerance and substrate utilization in diet-induced obese rats with insulin resistance. Mol Metab 2015;4:310-24.