Mustafa Gökhan ÜNSAL, Eyüp Murat YILMAZ, Erdem Barış CARTI, Çiğdem YENİŞEY, Ulaş Utku ŞEKERCİ

Effects of laparoscopy and laparotomy on stress and hemodynamics in experimental sepsis model in rats

Aim: Laparoscopic abdominal surgery leads to reduced inflammatory response compared with laparotomy. However, there iscontroversial approaches are present in abdominal diseases complicated by diffuse or localized peritonitis.Materials and Methods: Experimental cecal ligation and puncture method is used in the study. A total of 24 rats were used in thestudy. We divided all rats randomly four groups: control, sepsis, laparotomy and laparoscopy. After sepsis model was applied, wetook blood samples and lung and liver samples as well. In plasma samples we determined 8-OHdG, 3-Nitrotyrosine, HNE, PGF-2α viaELISA methods. The tissue samples were analyzed since the amount of blood samples were inadequate in all subjects. We measuredMPO activity to assess the neutrophil infiltration rates.Results: 3-NT mean values in laparotomy and laparoscopy groups were found to be almost the same while the difference betweensepsis and laparoscopy groups was statistically significant (p =0.015) and decreased in the group undergoing laparoscopy. MPOlevels obtained in rats underwent laparoscopy in liver tissue were found to be lower when compared with control and laparotomygroups and the difference between them was statistically significant. Also, MPO levels was found higher in sepsis laparotomy modelas compared sepsis laparoscopy models.Conclusion: Laparoscopy is accepted as golden standard in several elective procedures, since it is less stressful. Our results indicatethat laparoscopy is superior to laparotomy in terms of inflammatory response in sepsis and should be preferred in septic patients.

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