Ayse BAHAR CEYRAN, Huseyin UZUN, Sinan YOL, Suleyman ORMAN

Comparison of the microvessel density of gastric regions in normal and sleeve gastrectomized rats

Aim: Background: The aim of this study is to compare the microvessel densities of different normal gastric regions and to determine the effects of sleeve gastrectomy on the microvessel density of tissues alongside the remnant stomach.Material and Methods: Twenty male Wistar albino rats were divided into two groups. Rats in the control group were immediately sleeve gastrectomized under anesthesia and a wedge resection of the esophagogastric junction (EGJ), the fundus, corpus and antral gastric regions was performed, before sacrifice. Meanwhile, rats in the experiment group also underwent sleeve gastrectomy but were not sacrificed until the 5th postoperative day. At this point, gastric tissues alongside the sleeve gastrectomy area were then wedge-resected. The microvessel densities of these two groups were evaluated and compared.Results: When comparing distinctive gastric regions within the control group, the microvessel density of the esophagogastric junction was found to be less than in the corpus or fundus (20.04±4.45; 36.28±9.98, 39.4±9.57; p 0.05). The sleeve gastrectomy also had no significant effect on the microvessel densities at the esophagogastric junction or corpus (24.63±8.91, 22.24±7,63; p> 0.05).Conclusion: The esophagogastric junction has a lower microvessel density than the corpus in a normal stomach. Sleeve gastrectomy has no adverse effect on the microvessel density of the esophagogastric junction.

PDF

___

Thompson D, Wolf AM. The medical-care cost burden of obesity. Obes Rev 2001;2:189-97.
Courcoulas AP, Christian NJ, Belle SH, et al. Longitudinal Assessment of Bariatric Surgery (LABS) Consortium. Weight change and health outcomes at 3 years after bariatric surgery among individuals with severe obesity. JAMA 2013;310:2416-25.
Klimczak T, Klimczak J, Szewczyk T, et al. Endoscopic treatment of leaks after laparoscopic sleeve gastrectomy using MEGA esophageal covered stents. Surg Endosc 2018;32:2038-45.
Berger ER, Clements RH, Morton JM, et al. The Impact of Different Surgical Techniques on Outcomes in Laparoscopic Sleeve Gastrectomies: The First Report from the Metabolic and Bariatric Surgery Accreditation and Quality Improvement Program (MBSAQIP). Ann Surg 2016;264:464-73.
Nielsen JS, McNagny KM. Novel functions of the CD34 family. J Cell Sci 2008;121:3683-92.
Ramsden JD. Angiogenesis in the thyroid gland. J Endocrinol 2000;166:475-80.
Turner HE, Harris AL, Melmed S, et al. Angiogenesis in endocrine tumors. Endocr Rev 2003;24:600-32.
Yan B, Zhou Y, Feng S, et al. Β -Elemene-Attenuated Tumor Angiogenesis by Targeting Notch-1 in Gastric Cancer Stem-Like Cells. Evid Based Complement Alternat Med 2013;2013:268468.
Orman S, Karaman K, Basok BI, et al. Effects of thyroid hormone therapy on cut-surface healing of the remnant stomach with short-term weight loss alterations after sleeve gastrectomy. J Invest Surg 2017;16:1-10.
Vermeulen PB, Gasparini G, Fox SB, et al. Quantification of angiogenesis in solid human tumours: an international consensus on the methodology and criteria of evaluation. Eur J Cancer 1996;32:2474-84.
Baker RS, Foote J, Kemmeter P, et al. The science of stapling and leaks. Obes Surg 2004;14:1290-8.
Lossa A, Abdelgawad M, Watkins BM, at al. Leaks after laparoscopic sleeve gastrectomy: overview of pathogenesis and risk factors. Langenbecks Arch Surg 2016;401:757-66.
Tonnesen MG, Feng X, Clark RA. Angiogenesis in wound healing. J Invest Dermatol Symp Proc 2000;5:40-6.
Arcalís T, Carretero A, Navarro M, et al. Vasculogenesis and angiogenesis in the subcardinal venous plexus of quail mesonephros: spatial and temporal morphological analysis. Anat Embryol 2002;205:19-28.
Yoo JH, Shin JH, An MS, et al. adipose-tissue-derived stem cells enhance the healing of ischemic colonic anastomoses: an experimental study in rats. J Korean Soc Coloproctol 2012;28:132-9.
Bădescu A, Georgescu CV, Vere CC, et al. Correlations between Her2 oncoprotein, VEGF expression, MVD and clinicopathological parameters in gastric cancer. Rom J Morphol Embryol 2012;53:997-1005.
Liang P, Ren XC, Gao JB, Chen et al. Iodine concentration in spectral ct: assessment of prognostic determinants in patients with gastric adenocarcinoma. AJR Am J Roentgenol 2017;209:1033-8.
Zygoń J, Szajewski M, Kruszewski WJ, et al. VEGF, Flt-1, and microvessel density in primary tumors as predictive factors of colorectal cancer prognosis. Mol Clin Oncol 2017;6:243-8.
Liu H, Wu J, Liu XC, et al. Correlation between microvascular characteristics and the expression of MVD, IGF-1 and STAT3 in the development of colonic polyps carcinogenesis. Exp Ther Med 2017;13:49-54.
Haisan A, Rogojanu R, Croitoru C, et al. Digital microscopy assessment of angiogenesis in different breast cancer compartments. Biomed Res Int 2013;2013:286902.
Geng L, Luo D, Zhang HC, et al. Microvessel density at different levels of normal or injured bile duct in dogs and its surgical implications. Hepatobiliary Pancreat Dis Int 2011;10:83-7.
Wu P, Li L, Wang H, et al. Role of angiogenesis in chronic radiation proctitis: new evidence favoring inhibition of angiogenesis ex vivo. Dig Dis Sci 2018;63:113-25.
Erbil Y, Ozluk Y, Giriş M, et al. Effect of lugol solution on thyroid gland blood flow and microvessel density in the patients with Graves’ disease. J Clin Endocrinol Metab 2007;92:2182-9.
Jurowich C, Thalheimer A, Seyfried F, et al. Gastric leakage after sleeve gastrectomy-clinical presentation and therapeutic options. Langenbecks Arch Surg 2011;396:981-7.
Saber AA, Azar N, Dekal M, et al. Computed tomographic scan mapping of gastric wall perfusion and clinical implications. Am J Surg 2015;209:999-1006.
Rosenthal RJ; International Sleeve Gastrectomy Expert Panel, Diaz AA, et al. International sleeve gastrectomy expert panel consensus statement: best practice guidelines based on experience of >12,000 cases. Surg Obes Relat Dis 2012;8:8-19.