Hamit USLU, Oktay ÖZKAN, Dinçer ERDAĞ, Gözde ATİLA, Ali Bilgili

Streptozotosin ile diyabetes mellitus oluşturulan sıçanlarda Allium tuncelianum’un hiperglisemi ve böbrek ve karaciğer dokularında oksidatif stres üzerine etkileri

Bu çalışmada streptozotosin ile diyabetes mellitus oluşturulan sıçanlarda Allium tuncelianum ekstraktının hiperglisemi ve böbrek ve karaciğer dokularında oksidatif stres üzerine etkilerini araştırmak amaçlandı. Sıçanlar her bir grupta 10 hayvan olacak şekilde rastgele 4 gruba ayrıldı: Kontrol grubuna (C) periton içi (i.p.) fizyolojik tuzlu su, diyabetli kontrol (DC) grubuna i.p. tek doz 50 mg/kg streptozotosin (STZ), diyabet + insülin (D+I) grubuna i.p. tek doz 50 mg/kg STZ ve 28 gün süre ile deri altı (s.c.) 2 IU insülin (Levemir Flexpen), diyabet + Allium tuncelianum ekstrakt (D+AT) grubuna i.p. tek doz 50 mg/kg STZ ve 28 gün süre ile ağızdan 250 mg/kg Allium tuncelianum ekstraktı ugulandı. Serum glikozile hemoglobin (HbA1c), insülin düzeyleri ve böbrek ve karaciğer tiyobarbitürik asit reaktif maddeler (TBARS), süperoksit dismutaz (SOD) ve katalaz (CAT) aktiviteleri ELISA kitleri ile belirlendi. Kontrol grubuna göre diyabetli kontrol grubunda yüksek kan glikoz düzeyleri (p

Effects of Allium tuncelianum on hyperglycemia and oxidative stress in the kidney and liver tissues in rats with diabetes mellitus induced by streptozotocin

In this study, it was aimed to investigate the effects of Allium tuncelianum extract on hyperglycemia and oxidative stress in the kidney and liver tissues in rats with diabetes mellitus induced by streptozotocin. The rats were randomly divided into 4 groups with 10 animals in each group: Control group (C) was intraperitoneally (i.p.) treated with physiological saline solution, diabetic control (DC) group i.p. with a single dose of 50 mg/kg streptozotocin (STZ), diabetic + insulin (D+I) group i.p. with a single dose of 50 mg/kg STZ and subcutaneously (s.c.) with 2 IU insulin for 28 days (Levemir Flexpen), diabetic + Allium tuncelianum extract (D+AT) group i.p. with a single dose of 50 mg/kg STZ and orally with 250 mg/kg Allium tuncelianum extract for 28 days. The serum glycated hemoglobin (HbA1c), insulin levels and the kidney and liver thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), and catalase (CAT) activities were determined by using ELISA kits. Increased blood glucose levels (p

PDF

___

1. Abdultawab HS, Ayuob NN (2013): Can garlic oil ameliorate diabetes-induced oxidative stress in rat liver model? A correlated histological and biochemical study. Food Chem Toxicol, 59, 650-656.
2. Ahmed R (2005): The physiological and biochemical effects of diabetes on the balance between oxidative stress and antioxidant defense system. Med J Islam World Acad Sci, 15, 31-42.
3. Al-Qattan KK, Mansour MH, Thomson M, et al. (2016): Garlic decreases liver and kidney receptor for advanced glycation end products expression in experimental diabetes. Pathophysiology, 23, 135-145.
4. Anwar MM, Meki AR (2003): Oxidative stress in streptozotocin-induced diabetic rats: effects of garlic oil and melatonin. Comp Biochem Physiol A Mol Integr Physiol, 135, 539-547.
5. Aslan M, Orhan N, Deliorman Orhan D, et al. (2010): Hypoglycemic activity and antioxidant potential of some medicinal plants traditionally used in Turkey for diabetes. J Ethnopharmacol, 128, 384-389.
6. Augusti KT, Sheela CG (1996): Antiperoxide effect of S-allyl cysteine sulfoxide, an insulin secretagogue, in diabetic rats. Experientia, 52, 115-120.
7. Ayodhya S, Kusum S, Anjali S (2010): Hypoglycemic activity of different extracts of various herbal plants. Int J Ayurveda Res Pharm, 1, 212-224.
8. Badole SL, Ghule AE, Wagh NK (2013): Antidiabetic activity of Allium sativum. Bioactive food as dietary interventions for diabetes, http://dx.doi.org/10.1016/B978-0-12-397153-1.00015-9.
9. Baluchnejadmojarad T, Kiasalari Z, Afshin-Majd S, et al. (2017): S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. Eur J Pharmacol, 794, 69-76.
10. Bombicino SS, Iglesias DE, Rukavina-Mikusic IA, et al. (2017): Hydrogen peroxide, nitric oxide and ATP are molecules involved in cardiac mitochondrial biogenesis in Diabetes. Free Radic Biol Med, 112, 267-276.
11. Chawla A, Chawla R, Jaggi S (2016): Microvascular and macrovascular complications in diabetes mellitus: Distinct or continuum? Indian J Endocrinol Metab, 20, 546-551.
12. Chung SS, Ho EC, Lam KS, et al. (2003): Contribution of polyol pathway to diabetes-induced oxidative stress. J Am Soc Nephrol, 14, S233-236.
13. Dorotea D, Kwon G, Lee JH, et al. (2018): A pan-NADPH oxidase inhibitor ameliorates kidney injury in type 1 diabetic rats. Pharmacology, 102, 180-189.
14. Gdula-Argasinska J, Pasko P, Sulkowska-Ziaja K, et al. (2017): Anti-inflammatory activities of garlic sprouts, a source of α-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells. Acta Biochim Pol, 64, 551-559.
15. Hassan F, Saadia M, Sher M, et al. (2018): Comparative Effects of Aqueous and Organic Solvent Extracts of Garlic on Glucose Level and Lipid Profile of Diabetic Rats. Pak J Zool, 50, 389-392.
16. Jakus V (2000): The role of free radicals, oxidative stress and antioxidants systems in diabetic vascular disease. Bratisl Lek Listy, 101, 541-551.
17. Kandemir FM, Ozkaraca M, Küçükler S, et al. (2018): Preventive effects of hesperidin on diabetic nephropathy induced by streptozotocin via modulating TGF-β1 and oxidative DNA damage. Toxin Rev, 37, 287-293.
18. Katsarou A, Gudbjörnsdottir S, Rawshani A, et al. (2017): Type 1 diabetes mellitus. Nat Rev Dis Primers, 3, 17016.
19. Kaur G, Padiya R, Adela R, et al. (2016): Garlic and resveratrol attenuate diabetic complications, loss of β-cells, pancreatic and hepatic oxidative stress in streptozotocin-induced diabetic rats. Front Pharmacol, 7, 360.
20. Lohwasser C, Neureiter D, Popov Y, et al. (2009): Role of the receptor for advanced glycation end products in hepatic fibrosis. World J Gastroenterol, 15, 5789-5798.
21. Malik S, Suchal K, Khan SI, et al. (2017): Apigenin ameliorates streptozotocin-induced diabetic nephropathy in rats via MAPK-NF-κB-TNF-α-and TGF-β1-MAPK-fibronectin pathways. Am J Physiol Renal Physiol, 313, F414-F422.
22. Maritim A, Sanders R, Watkins J (2003): Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol, 17, 24-38.
23. Metiner K, Ozkan O, Ak S (2016): In vitro antibacterial and antifungal activity of ethanolic Allium tuncelianum extract. Global Veterinaria, 16, 26-30.
24. Morihara N, Hayama M, Fuji H (2011): Aged garlic extract scavenges superoxide radicals. Plant Foods Hum Nutr, 66, 17-21.
25. Nasiri A, Ziamajidi N, Abbasalipourkabir R, et al. (2017): Beneficial effect of aqueous garlic extract on inflammation and oxidative stress status in the kidneys of type 1 diabetic rats. Indian J Clin Biochem, 32, 329-336.
26. Niedowicz DM, Daleke DL (2005): The role of oxidative stress in diabetic complications. Cell Biochem Biophys, 43, 289-330.
27. Ozkan O, Gül S, Kart A, et al. (2013): In vitro antimutagenicity of Allium tuncelianum ethanol extract against induction of chromosome aberration by mutagenic agent mitomycine C. Kafkas Univ Vet Fak Derg, 19, 259-262.
28. Patel DK, Prasad SK, Kumar R, et al. (2012). An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed, 2, 320-330.
29. Rajani Kanth V, Uma Maheswara Reddy P, Raju TN (2008): Attenuation of streptozotocin-induced oxidative stress in hepatic and intestinal tissues of Wistar rat by methanolic-garlic extract. Acta Diabetol, 45, 243-251.
30. Rodriguez V, Plavnik L, Tolosa de Talamoni N (2018): Naringin attenuates liver damage in streptozotocin-induced diabetic rats. Biomed Pharmacother, 105, 95-102.
31. Roe ND, Thomas DP, Ren J (2011): Inhibition of NADPH oxidase alleviates experimental diabetes-induced myocardial contractile dysfunction. Diabetes Obes Metab, 13, 465-473.
32. Roh SS, Kwon OJ, Yang JH, et al. (2016): Allium hookeri root protects oxidative stress-induced inflammatory responses and β-cell damage in pancreas of streptozotocin-induced diabetic rats. BMC Complement Altern Med, 16, 63.
33. Saravanan G, Ponmurugan P (2010): Beneficial effect of S-allylcysteine (SAC) on blood glucose and pancreatic antioxidant system in streptozotocin diabetic rats. Plant Foods Hum Nutr, 65, 374-378.
34. Shiju TM, Rajesh NG, Viswanathan P (2013): Renoprotective effect of aged garlic extract in streptozotocin-induced diabetic rats. Indian J Pharmacol, 45, 18-23.
35. Sivaraman K, Senthilkumar GP, Sankar P, et al. (2013): Attenuation of oxidative stress, inflammation and insulin resistance by Allium sativum in fructose-fed male rats. J Clin Diagn Res, 7, 1860-1862.
36. Sultana MR, Bagul PK, Katare PB, et al. (2016). Garlic activates SIRT-3 to prevent cardiac oxidative stress and mitochondrial dysfunction in diabetes. Life Sci, 164, 42-51.
37. Takım K (2015): Tunceli Dağ Sarımsağı’nın (Allium tuncelianum) in vitro antioksidan kapasitesinin ölçülmesi, ratlarda antioksidan enzim aktiviteleri üzerine etkisi ve antikanser özelliğinin belirlenmesi. T.C. İnönü Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi.
38. Thomson M, Al-Qattan KK, Divya JS, et al. (2016): Anti-diabetic and anti-oxidant potential of aged garlic extract (AGE) in streptozotocin-induced diabetic rats. BMC Complement Altern Med, 16, 17.
39. Tomita T (2017): Apoptosis of pancreatic β-cells in type 1 diabetes. Bosn J Basic Med Sci, 17, 183-193.
40. Uslu H, Atila Uslu G, Özen H, et al. (2018): Effects of different doses of Prunus laurocerasus L. leaf extract on oxidative stress, hyperglycemia and hyperlipidaemia induced by type I diabetes. IJTK, 17, 430-436.
41. Varga ZV, Giricz Z, Liaudet L, et al. (2015): Interplay of oxidative, nitrosative/nitrative stress, inflammation, cell death and autophagy in diabetic cardiomyopathy. Biochim Biophys Acta, 1852, 232-42.
42. Zahid Ashraf M, Hussain ME, Fahim M (2005): Antiatherosclerotic effects of dietary supplementations of garlic and turmeric: Restoration of endothelial function in rats. Life Sci, 77, 837-857.