Pınar Alkım ULUTAS, Funda KIRAL, Ulvi Reha FIDANCI

Lipid perioxidation and antioxidant enzymes in rats exposed to cigarette smoke

Bu çalışmada uzun süre sigara dumanına maruz bırakılan sıçanlarda oksidatif metabolizma incelenmiştir. Çalışmada toplam 44 sağlıklı sıçan (22 erkek, 22 dişi) kullanılmıştır. Kontrol grubunda 11 erkek ve 11 dişi, sigara grubunda 11 erkek ve 11 dişi rat bulunmaktadır. Pasif dumana maruz bırakılan sıçanlara 16 hafta boyunca, haftada 5 gün, günde 120 dakika duman uygulanmıştır. Bu periyodun sonunda tüm hayvanlardan kan örnekleri toplanmıştır. Plazma MDA ve askorbik asit analizleri hemen yapılmıştır. Eritrositlerde hemoglobin düzeyleri ve GSH-px, SOD, CAT aktiviteleri belirlenmiştir. Sonuçlar student t-testi ile değerlendirilmiştir. Kontrol grubu ile karşılaştırıldığında erkek ve dişi sıçanların plazma MDA düzeyi ile GSH-px ve CAT aktivitelerinin yüksek, askorbik asit düzeylerinin düşük olduğu görülmüştür. SOD aktivitesi açısından ise her iki grup arasında istatistiksel farka rastlanmamıştır. Sonuç olarak, yüksek plazma lipid peroksidasyonu ve düşük askorbik asit düzeylerinin sigaranın patojenitesini arttırdığı düşünülmektedir.

Sigara dumanına maruz bırkılan sıçanlarda lipid peroksidasyonu ve antioksidan enzimler

Oxidative metabolism in rats exposed to long term cigarette smoking was investigated in this study. Fourty four rats (22 males and 22 females) were used in this study. 11 males and 11 female rats were assigned into cigarette smoke group and 11 male and 11 female rats were used as control group. The rats assigned into passive smoking were exposed to smoke in a unit for 120 minutes a day for five days a week during 16 weeks. At the end of this period, blood samples were collected from all rats. Plasma MDA and ascorbic acid analysis were performed immediately. Haemoglobin levels and GSH-px, SOD and CAT activities were determined in erythrocytes. The results were evaluated to student t-test. It was observed that plazma MDA levels and GSH-px, and CAT activities were high and plasma ascorbic acid levels were low compared with the control group in male and female rats. SOD activities showed no significant differences. As a result, it was concluded that high plasma lipid peroxidation and low ascorbic acid concentrations may be involved in the pathogenicity of cigarette.

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1. Andersen R, Theron AJ, Richards GA, Myers MS, Rensberg JV (1991): Passive smoking by human sensitizes circulating neutrophils. Am Rev Respir Dis, 144, 570-574.
2. Behr J, Nowak D (2002): Tobacco smoke and respiratory disease. Eur Respir Monograph, 21, 161-179.
3. Bolzan AD, Bianchi MS, Bianchi NO (1997): Superoxide dismutase, catalase and glutathione peroxidase activities in human blood: influence of sex, age and cigarette smoking. Clin Biochem, 30, 449-454.
4. Clara JG, Coelho C, Breintenfelt L, Siqueira C (2001): Acute effects of tobacco and vascular risk modulated by genetic factors. Rev Port Cardiol, 20, 103-109.
5. Crowley-Weber CL, Dvorakova K, Crowley C, Bernstesin H, breinstein C, Garewal H, Payne CM (2003): Nicotine increases oxidative stress, activates NFkappaB and GRP78, induces apoptosis and sensities cells to genotoxic/xenobiotic stresses by a multiple stress inducer, deoxycholate: relevance to colon carcinogenesis. Chem Biol Interact, 145, 53-66.
6. Demir S, Özkurt S, Köseoğlu M, Enli Y, Aslan D, Gümüşsu. N (2001): Sigara içenlerde plazma lipid peroksidasyonu. Solunum, 3, 57-59.
7. Doll R (1996): Cancers weakly releated to smoking. Br Med Bull, 52, 35-49.
8. Fisher EB, Debra JR, Morgan DG, Rehberg H, Rost K (1990): Smoking and smoking cessation. Am Rev Respir Dis, 142, 702-720.
9. Gültekin F, Gökduman A, Özgüner F, akdoğan M, Sütçü R, Delibaş N (2001): Sigara içenlerin antioksidan savunma sistemindeki değişmeler. Tuberk Toraks Derg, 49, 259-264.
10. Halliwell B, (1994): Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet, 344, 721-724.
11. Hunninghake WG, Crystal RG (1993): Cigarette smoking and lung destruction. accumulation of neutrophis in the lungs of cigarette smoking. Am Rev Respir Dis, 128, 833-838.
12. Kalra J, Chaudhary AK, Prasad K (1991): Increased production of oxygen free radicals in cigarette smokers. Int J Exp Path, 72, 1-7.
13. Kim HS, Lee BM (2001): Protective effects of antioxidant supplementation on plasma lipid peroxidation in smokers. J Toxicol Environ Health, 24, 583-98.
14. Kway A (1978): A simple colorimetric method for ascorbic acid determination in blood plasma. Clin Chim Acta, 86, 153-157.
15. Luck H (1965): Catalase. 855-884, In: Methods in Analysis, HU Bergmeyer (Ed), Academic Press, London. 16. Marrow JD, Frei B, Longwire AW, Gaziano JM, Lynch SM, Shyr J (1995): Increase in circulating products of lipid peroxidation in smokers. Smoking as a cause of oxidative damage. N Eng J Med, 332, 1198-203.
17. Mccusker K, Hoidal J (1990): Selective increase of antioxidant enzyme activity in the alveolar macrophages from cigarette smokers and smoke-exposed hamsters. Am Rev Respir Dis, 141, 678-682.
18. Nadiger HA, Mathew CA, Sadasivudu B (1987): Serum malondialdehyde (TBA reactive substance) levels in cigarette smokers. Atherosclerosis, 64, 71-73.
19. Naidu KA (2003): Vitamin C in human healty and disease is still a mystery? an overwiev. Nutr J, 21, 2-7.
20. Ozan E, Çolakoğlu N, Sönmez MF, Ozan S, Yılmaz S, Taşdemir B, Ozan G (2005): Sigara inhalasyonunun trakeada oluşturduğu yapısal değişiklikler üzerine melatonin ve C vitamininin etkileri. Fırat Tıp Derg, 10, 40-44.
21. Paglia DE, Valentine WN (1967): Studies on the qualitative and quantitative characterization of erytrocyte glutathione peroxidase. J Lab Clin Med, 70, 158-169.
22. Prasad K, Kalra J (1989): Experimental atherosclerosis and oxygen free radicals. Angiology, 40, 835-843.
23. Rahman I, Macnee W (1996): Oxidant-antioxidant imbalance in smokers and chronic obstructive pulmonary disease. Thorax, 51, 348-350.
24. Sohn HO, Lim HB, Lee DW, Kim YT (1993): Effect of subchronic administration of antioxidants aganist cigarette smoke exposure in rats. Arch Toxicol, 67, 667-673.
25. Steinberg FM, Chait A (1998): Antioxidant vitamin supplementation and lipid peroxidation in smokers. Am J Clin Nutr, 68, 319-27.
26. Sun Y, Oberley LW, Li YA (1988): A simple for clinical assay of superoxide dismutase. Clin Chem, 34, 397-500.
27. Tietz WN (1987): Measurement of plasma haemoglobin. 803-813. In: Fundamental of Clinical Chemistry. Third ed, Saunders Company, Philadelphia.
28. Winterborn CC, Hawkins RE, Brain M, Carrel W (1975): The estimation of red cell superoxide dismutase activity. J Lab Clin Med, 55, 337-341.
29. Yıldız D (2004): Nicotine, its metabolism an overview of its biological effects. Toxicon, 43, 619-32.
30. Yoshioka T, Kawada K, Shimada T (1979): Lipid peroxidation in maternal and cord blood and protective mechanism against active-oxygen toxicity in the blood. Am J Obstet Gynecol 135, 372-379.
31. Yöntem M, Çevrim EE, Kaleli S (2002): Sigara içen ve içmeyen kişilerde süperoksit dismutaz, glutatyon redüktaz, ferritin ve hemoglobin düzeylerinin araştırılması. Süleyman Demirel Üniv Fen Bil Enst Derg, 6, 79-86.